RGD-independent binding of integrin α9β1 to the ADAM-12 and -15 disintegrin domains mediates cell-cell interaction

Koji Eto, Wilma Puzon-McLaughlin, Dean Sheppard, Atsuko Sehara-Fujisawa, Xi Ping Zhang, Yoshikazu Takada

Research output: Contribution to journalArticle

192 Citations (Scopus)

Abstract

ADAMs (a disintegrin and metalloproteases) mediate several important processes (e.g. tumor necrosis factor-α release, fertilization, and myoblast fusion). The ADAM disintegrin domains generally lack RGD motifs, and their receptors are virtually unknown. Here we show that integrin α9β1 specifically interacts with the recombinant ADAMs-12 and -15 disintegrin domains in an RGD-independent manner. We also show that interaction between ADAM-12 or -15 and α9β1 supports cell-cell interaction. Interestingly, the cation requirement and integrin activation status required for α9β1/ADAM-mediated cell adhesion and cell-cell interaction is similar to those required for known integrin-extracellular matrix interaction. These results are quite different from recent reports that ADAM-2/α6β1 interaction during sperm/egg fusion requires an integrin activation status distinct from that for extracellular matrix interaction. These results suggest that α9β1 may be a major receptor for ADAMs that lack RGD motifs, and that, considering a wide distribution of ADAMs and α9β1, this interaction may be of potential biological and pathological significance.

Original languageEnglish (US)
Pages (from-to)34922-34930
Number of pages9
JournalJournal of Biological Chemistry
Volume275
Issue number45
DOIs
StatePublished - Nov 10 2000
Externally publishedYes

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Disintegrins
Metalloproteases
Integrins
Cell Communication
Extracellular Matrix
Fusion reactions
Chemical activation
Sperm-Ovum Interactions
Myoblasts
Cell adhesion
Fertilization
Cell Adhesion
Cations
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • Biochemistry

Cite this

RGD-independent binding of integrin α9β1 to the ADAM-12 and -15 disintegrin domains mediates cell-cell interaction. / Eto, Koji; Puzon-McLaughlin, Wilma; Sheppard, Dean; Sehara-Fujisawa, Atsuko; Zhang, Xi Ping; Takada, Yoshikazu.

In: Journal of Biological Chemistry, Vol. 275, No. 45, 10.11.2000, p. 34922-34930.

Research output: Contribution to journalArticle

Eto, Koji ; Puzon-McLaughlin, Wilma ; Sheppard, Dean ; Sehara-Fujisawa, Atsuko ; Zhang, Xi Ping ; Takada, Yoshikazu. / RGD-independent binding of integrin α9β1 to the ADAM-12 and -15 disintegrin domains mediates cell-cell interaction. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 45. pp. 34922-34930.
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