Reversible and persistent consequences of copper deficiency in developing mice

Deborah S. Arce, Carl L Keen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The effect of maternal copper (Cu) deficiency on various proteins was studied to determine if the changes were reversible or persistent with Cu repletion. The functional consequences of these alterations were assessed by exposing the animals to an oxidative stress (endotoxin), and by measuring the formation of benzo[a] pyrene-DNA adducts in vitro. Throughout gestation and lactation, mice were fed a Control diet (10 μg Cu/g diet) or a Low Cu diet (1 μg Cu/g diet). On day 18, the offspring were killed or switched to the Control diet and killed on day 42 following a single injection of saline or endotoxin on day 41. In day-18 offspring, Cu deficiency resulted in decreased hematocrit values, ceruloplasmin activity, liver and tissue Cu levels, and metallothionein concentrations. Cu repletion restored all but metallothionein levels. Early Cu deficiency led to higher brain CuZn superoxide dismutase activity on day 42, and higher levels of brain thiobarbituric acid reactive substances (TBARS) in endotoxin-treated mice. Liver TBARS were lower in day-18 Low Cu offspring and in day-42 Low Cu offspring treated with endotoxin than age-matched Controls. Cytochrome P-450 concentrations were lower in Low Cu, endotoxin-treated males than in Controls. These results show that Cu deficiency-mediated alterations during early development are not immediately reversed with Cu repletion.

Original languageEnglish (US)
Pages (from-to)211-221
Number of pages11
JournalReproductive Toxicology
Volume6
Issue number3
DOIs
StatePublished - 1992

Fingerprint

Nutrition
Endotoxins
Copper
Diet
Thiobarbituric Acid Reactive Substances
Metallothionein
Liver
Brain
Ceruloplasmin
Oxidative stress
Hematocrit
Lactation
Cytochrome P-450 Enzyme System
Superoxide Dismutase
Animals
Oxidative Stress
Mothers
Tissue
Pregnancy
Injections

Keywords

  • copper
  • cytochrome P-450
  • development
  • endotoxin
  • lipid peroxidation
  • metallothionein
  • superoxide dismutase
  • TBARS

ASJC Scopus subject areas

  • Toxicology

Cite this

Reversible and persistent consequences of copper deficiency in developing mice. / Arce, Deborah S.; Keen, Carl L.

In: Reproductive Toxicology, Vol. 6, No. 3, 1992, p. 211-221.

Research output: Contribution to journalArticle

Arce, Deborah S. ; Keen, Carl L. / Reversible and persistent consequences of copper deficiency in developing mice. In: Reproductive Toxicology. 1992 ; Vol. 6, No. 3. pp. 211-221.
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