Reversibility of apical dendritic retraction in the rat medial prefrontal cortex following repeated stress

Jason J. Radley, Anne B. Rocher, William G.M. Janssen, Patrick R. Hof, Bruce S. McEwen, John Morrison

Research output: Contribution to journalArticle

202 Citations (Scopus)

Abstract

Apical dendritic retraction and axospinous synapse loss in the medial prefrontal cortex (PFC) are structural alterations that result from repeated restraint stress. Such changes in this brain region may be associated with impaired working memory, altered emotionality, and inability to regulate hypothalamic-pituitary adrenal activity, which in turn may underlie stress-related mental illnesses. In the present study, we examined the persistence of these stress-induced dendritic alterations in the medial PFC following the cessation of stress. Animals received either daily restraint stress for a 3-week period and were then allowed to recover for another 3 weeks, restraint stress for 3 or 6 weeks, or no restraint. Following perfusion and fixation, intracellular iontophoretic injections of Lucifer Yellow were performed in layer II/III pyramidal neurons in slices from the medial PFC, and apical and basal dendritic arbors were reconstructed in three dimensions. We observed a significant reduction in apical dendritic length and branch number following 3 or 6 weeks of repeated stress compared to 3-week stress/3-week recovery. These results suggest that stress-induced dendritic plasticity in the medial PFC is reversible and may have implications for the functional recovery of medial PFC function following prolonged psychological stress.

Original languageEnglish (US)
Pages (from-to)199-203
Number of pages5
JournalExperimental Neurology
Volume196
Issue number1
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Fingerprint

Prefrontal Cortex
Pyramidal Cells
Short-Term Memory
Psychological Stress
Synapses
Perfusion
Injections
Brain

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Reversibility of apical dendritic retraction in the rat medial prefrontal cortex following repeated stress. / Radley, Jason J.; Rocher, Anne B.; Janssen, William G.M.; Hof, Patrick R.; McEwen, Bruce S.; Morrison, John.

In: Experimental Neurology, Vol. 196, No. 1, 01.11.2005, p. 199-203.

Research output: Contribution to journalArticle

Radley, Jason J. ; Rocher, Anne B. ; Janssen, William G.M. ; Hof, Patrick R. ; McEwen, Bruce S. ; Morrison, John. / Reversibility of apical dendritic retraction in the rat medial prefrontal cortex following repeated stress. In: Experimental Neurology. 2005 ; Vol. 196, No. 1. pp. 199-203.
@article{98ccc5ae36134aac843f0f8caeb65b37,
title = "Reversibility of apical dendritic retraction in the rat medial prefrontal cortex following repeated stress",
abstract = "Apical dendritic retraction and axospinous synapse loss in the medial prefrontal cortex (PFC) are structural alterations that result from repeated restraint stress. Such changes in this brain region may be associated with impaired working memory, altered emotionality, and inability to regulate hypothalamic-pituitary adrenal activity, which in turn may underlie stress-related mental illnesses. In the present study, we examined the persistence of these stress-induced dendritic alterations in the medial PFC following the cessation of stress. Animals received either daily restraint stress for a 3-week period and were then allowed to recover for another 3 weeks, restraint stress for 3 or 6 weeks, or no restraint. Following perfusion and fixation, intracellular iontophoretic injections of Lucifer Yellow were performed in layer II/III pyramidal neurons in slices from the medial PFC, and apical and basal dendritic arbors were reconstructed in three dimensions. We observed a significant reduction in apical dendritic length and branch number following 3 or 6 weeks of repeated stress compared to 3-week stress/3-week recovery. These results suggest that stress-induced dendritic plasticity in the medial PFC is reversible and may have implications for the functional recovery of medial PFC function following prolonged psychological stress.",
author = "Radley, {Jason J.} and Rocher, {Anne B.} and Janssen, {William G.M.} and Hof, {Patrick R.} and McEwen, {Bruce S.} and John Morrison",
year = "2005",
month = "11",
day = "1",
doi = "10.1016/j.expneurol.2005.07.008",
language = "English (US)",
volume = "196",
pages = "199--203",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Reversibility of apical dendritic retraction in the rat medial prefrontal cortex following repeated stress

AU - Radley, Jason J.

AU - Rocher, Anne B.

AU - Janssen, William G.M.

AU - Hof, Patrick R.

AU - McEwen, Bruce S.

AU - Morrison, John

PY - 2005/11/1

Y1 - 2005/11/1

N2 - Apical dendritic retraction and axospinous synapse loss in the medial prefrontal cortex (PFC) are structural alterations that result from repeated restraint stress. Such changes in this brain region may be associated with impaired working memory, altered emotionality, and inability to regulate hypothalamic-pituitary adrenal activity, which in turn may underlie stress-related mental illnesses. In the present study, we examined the persistence of these stress-induced dendritic alterations in the medial PFC following the cessation of stress. Animals received either daily restraint stress for a 3-week period and were then allowed to recover for another 3 weeks, restraint stress for 3 or 6 weeks, or no restraint. Following perfusion and fixation, intracellular iontophoretic injections of Lucifer Yellow were performed in layer II/III pyramidal neurons in slices from the medial PFC, and apical and basal dendritic arbors were reconstructed in three dimensions. We observed a significant reduction in apical dendritic length and branch number following 3 or 6 weeks of repeated stress compared to 3-week stress/3-week recovery. These results suggest that stress-induced dendritic plasticity in the medial PFC is reversible and may have implications for the functional recovery of medial PFC function following prolonged psychological stress.

AB - Apical dendritic retraction and axospinous synapse loss in the medial prefrontal cortex (PFC) are structural alterations that result from repeated restraint stress. Such changes in this brain region may be associated with impaired working memory, altered emotionality, and inability to regulate hypothalamic-pituitary adrenal activity, which in turn may underlie stress-related mental illnesses. In the present study, we examined the persistence of these stress-induced dendritic alterations in the medial PFC following the cessation of stress. Animals received either daily restraint stress for a 3-week period and were then allowed to recover for another 3 weeks, restraint stress for 3 or 6 weeks, or no restraint. Following perfusion and fixation, intracellular iontophoretic injections of Lucifer Yellow were performed in layer II/III pyramidal neurons in slices from the medial PFC, and apical and basal dendritic arbors were reconstructed in three dimensions. We observed a significant reduction in apical dendritic length and branch number following 3 or 6 weeks of repeated stress compared to 3-week stress/3-week recovery. These results suggest that stress-induced dendritic plasticity in the medial PFC is reversible and may have implications for the functional recovery of medial PFC function following prolonged psychological stress.

UR - http://www.scopus.com/inward/record.url?scp=23444432520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23444432520&partnerID=8YFLogxK

U2 - 10.1016/j.expneurol.2005.07.008

DO - 10.1016/j.expneurol.2005.07.008

M3 - Article

VL - 196

SP - 199

EP - 203

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

IS - 1

ER -