Retrograde release of endocannabinoids inhibits presynaptic GABA release to second-order baroreceptive neurons in NTS

Chao-Yin Chen, Ann C. Bonham, Caron Dean, Francis A. Hopp, Cecilia J. Hillard, Jeanne L. Seagard

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In prior studies, we found that activation of cannabinoid-1 receptors in the nucleus tractus solitarii (NTS) prolonged baroreflex-induced sympathoinhibition in rats. In many regions of the central nervous system, activation of cannabinoid-1 receptors presynaptically inhibits γ-aminobutyric acid (GABA) release, disinhibiting postsynaptic neurons. To determine if cannabinoid-1 receptor-mediated presynaptic inhibition of GABA release occurs in the NTS, we recorded miniature inhibitory postsynaptic currents in anatomically identified second-order baroreceptive NTS neurons in the presence of ionotropic glutamate receptor antagonists and tetrodotoxin. The cannabinoid-1 receptor agonists, WIN 55212-2 (0.3-30 μM) and methanandamide (3 μM) decreased the frequency of miniature inhibitory postsynaptic currents in a concentration-dependent manner, an effect that was blocked by the cannabinoid-1 receptor antagonist, N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM 251, 5 μM). Importantly, depolarization of second-order baroreceptive neurons decreased the frequency of miniature inhibitory postsynaptic currents; an effect which was blocked by the cannabinoid-1 receptor antagonist. The data indicate that depolarization of second-order baroreceptive NTS neurons induces endocannabinoid release from the neurons, leading to activation of presynaptic cannabinoid-1 receptors, inhibition of GABA release and subsequent enhanced baroreflex signaling in the NTS. The data suggest that endocannabinoid signaling in the NTS regulates short-term synaptic plasticity and provide a mechanism for endocannabinoid modulation of central baroreflex control.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalAutonomic Neuroscience: Basic and Clinical
Volume158
Issue number1-2
DOIs
StatePublished - Dec 8 2010

Fingerprint

Endocannabinoids
Solitary Nucleus
gamma-Aminobutyric Acid
Cannabinoid Receptors
Neurons
Inhibitory Postsynaptic Potentials
Baroreflex
Cannabinoid Receptor Antagonists
Cannabinoid Receptor Agonists
Aminobutyrates
Ionotropic Glutamate Receptors
Excitatory Amino Acid Antagonists
Neuronal Plasticity
Tetrodotoxin
Central Nervous System

Keywords

  • Baroreflex
  • Brainstem
  • Electrophysiology
  • Rat

ASJC Scopus subject areas

  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems

Cite this

Retrograde release of endocannabinoids inhibits presynaptic GABA release to second-order baroreceptive neurons in NTS. / Chen, Chao-Yin; Bonham, Ann C.; Dean, Caron; Hopp, Francis A.; Hillard, Cecilia J.; Seagard, Jeanne L.

In: Autonomic Neuroscience: Basic and Clinical, Vol. 158, No. 1-2, 08.12.2010, p. 44-50.

Research output: Contribution to journalArticle

Chen, Chao-Yin ; Bonham, Ann C. ; Dean, Caron ; Hopp, Francis A. ; Hillard, Cecilia J. ; Seagard, Jeanne L. / Retrograde release of endocannabinoids inhibits presynaptic GABA release to second-order baroreceptive neurons in NTS. In: Autonomic Neuroscience: Basic and Clinical. 2010 ; Vol. 158, No. 1-2. pp. 44-50.
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