Retinoid X and retinoic acid receptors interact with transcription factor II-B by distinct mechanisms

Hongwu Chen, Martin L. Privalsky

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Nuclear hormone receptors are believed to modulate target gene expression by interacting with the general transcriptional machinery of the cell. We demonstrate here that two otherwise closely related members of the nuclear hormone receptor family, retinoid acid receptors (RARs) and retinoid X receptors (RXRs), exhibit significant differences in their interactions with the transcriptional machinery. RARs display a strong constitutive interaction with transcription factor II-B (TFIIB) that requires the TFIIB C-terminus, whereas RXR exhibits a weaker, hormone-stimulated interaction with the TFIIB that maps outside of the TFIIB C-terminus. Use of a dominant-negative mutant of TFIIB suggests that the TFIIB interaction is essential for full transcriptional activation by RXR.

Original languageEnglish (US)
Pages (from-to)55-61
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume129
Issue number1
DOIs
StatePublished - Apr 25 1997

Keywords

  • Nuclear hormone receptors
  • Retinoid receptors
  • Transcription factors
  • Transcriptional regulation

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Fingerprint Dive into the research topics of 'Retinoid X and retinoic acid receptors interact with transcription factor II-B by distinct mechanisms'. Together they form a unique fingerprint.

  • Cite this