Retention of intratumor injections of cisplatinum in murine tumors and the impact on laser thermal therapy for cancer treatment

Sarath Kanekal, Jayne S Joo, Michael Bublik, Adir Bababeygy, Christopher Loh, Dan J. Castro, Joel A. Sercarz, Marcos B. Paiva

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recent studies using murine models of human squamous cell carcinoma (SCCA) have revealed a significant improvement in survival and cure rate of animals transplanted with human SCCA when treated with a combination of intratumor injections of chemotherapy and laser induced thermal therapy (LITT). These preliminary results suggest that this novel combination therapy may lead to improved clinical response compared to either treatment modality alone. Using a murine model of human SCCA we investigated two different modes of intratumor injection of cisplatin: a sustained-release cisplatin gel implant (CDDP/gel) versus cisplatin in solution (CDDP) at varying doses (range 1-3 mg/ml). In addition, we tested CDDP/gel combined with LITT. Results showed optimal drug concentration (30-300 nM) at tumor margins up to 4 h after injection of CDDP/gel implant compared to 3 nM at 5 min after injection with CDDP solution. Combined CDDP/gel and laser therapy significantly decreased tumor volume (P < 0.05), with recurrence in only 25% of animals tested, compared to 78% tumor regrowth after LITT alone. These results suggest that laser chemotherapy may be an effective treatment for head and neck SCCA.

Original languageEnglish (US)
Pages (from-to)279-284
Number of pages6
JournalEuropean Archives of Oto-Rhino-Laryngology
Volume266
Issue number2
DOIs
StatePublished - Feb 2009
Externally publishedYes

Fingerprint

Laser Therapy
Hot Temperature
Gels
Injections
Lasers
Cisplatin
Squamous Cell Carcinoma
Neoplasms
Therapeutics
Drug Therapy
Tumor Burden
Recurrence
Pharmaceutical Preparations

Keywords

  • Biodistribution
  • Cisplatin
  • Intratumor-injections
  • Laser-induced thermal therapy
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Retention of intratumor injections of cisplatinum in murine tumors and the impact on laser thermal therapy for cancer treatment. / Kanekal, Sarath; Joo, Jayne S; Bublik, Michael; Bababeygy, Adir; Loh, Christopher; Castro, Dan J.; Sercarz, Joel A.; Paiva, Marcos B.

In: European Archives of Oto-Rhino-Laryngology, Vol. 266, No. 2, 02.2009, p. 279-284.

Research output: Contribution to journalArticle

Kanekal, Sarath ; Joo, Jayne S ; Bublik, Michael ; Bababeygy, Adir ; Loh, Christopher ; Castro, Dan J. ; Sercarz, Joel A. ; Paiva, Marcos B. / Retention of intratumor injections of cisplatinum in murine tumors and the impact on laser thermal therapy for cancer treatment. In: European Archives of Oto-Rhino-Laryngology. 2009 ; Vol. 266, No. 2. pp. 279-284.
@article{d46d5a109f1a4a16a049e5c5bd153707,
title = "Retention of intratumor injections of cisplatinum in murine tumors and the impact on laser thermal therapy for cancer treatment",
abstract = "Recent studies using murine models of human squamous cell carcinoma (SCCA) have revealed a significant improvement in survival and cure rate of animals transplanted with human SCCA when treated with a combination of intratumor injections of chemotherapy and laser induced thermal therapy (LITT). These preliminary results suggest that this novel combination therapy may lead to improved clinical response compared to either treatment modality alone. Using a murine model of human SCCA we investigated two different modes of intratumor injection of cisplatin: a sustained-release cisplatin gel implant (CDDP/gel) versus cisplatin in solution (CDDP) at varying doses (range 1-3 mg/ml). In addition, we tested CDDP/gel combined with LITT. Results showed optimal drug concentration (30-300 nM) at tumor margins up to 4 h after injection of CDDP/gel implant compared to 3 nM at 5 min after injection with CDDP solution. Combined CDDP/gel and laser therapy significantly decreased tumor volume (P < 0.05), with recurrence in only 25{\%} of animals tested, compared to 78{\%} tumor regrowth after LITT alone. These results suggest that laser chemotherapy may be an effective treatment for head and neck SCCA.",
keywords = "Biodistribution, Cisplatin, Intratumor-injections, Laser-induced thermal therapy, Squamous cell carcinoma",
author = "Sarath Kanekal and Joo, {Jayne S} and Michael Bublik and Adir Bababeygy and Christopher Loh and Castro, {Dan J.} and Sercarz, {Joel A.} and Paiva, {Marcos B.}",
year = "2009",
month = "2",
doi = "10.1007/s00405-008-0736-3",
language = "English (US)",
volume = "266",
pages = "279--284",
journal = "European Archives of Oto-Rhino-Laryngology",
issn = "0937-4477",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Retention of intratumor injections of cisplatinum in murine tumors and the impact on laser thermal therapy for cancer treatment

AU - Kanekal, Sarath

AU - Joo, Jayne S

AU - Bublik, Michael

AU - Bababeygy, Adir

AU - Loh, Christopher

AU - Castro, Dan J.

AU - Sercarz, Joel A.

AU - Paiva, Marcos B.

PY - 2009/2

Y1 - 2009/2

N2 - Recent studies using murine models of human squamous cell carcinoma (SCCA) have revealed a significant improvement in survival and cure rate of animals transplanted with human SCCA when treated with a combination of intratumor injections of chemotherapy and laser induced thermal therapy (LITT). These preliminary results suggest that this novel combination therapy may lead to improved clinical response compared to either treatment modality alone. Using a murine model of human SCCA we investigated two different modes of intratumor injection of cisplatin: a sustained-release cisplatin gel implant (CDDP/gel) versus cisplatin in solution (CDDP) at varying doses (range 1-3 mg/ml). In addition, we tested CDDP/gel combined with LITT. Results showed optimal drug concentration (30-300 nM) at tumor margins up to 4 h after injection of CDDP/gel implant compared to 3 nM at 5 min after injection with CDDP solution. Combined CDDP/gel and laser therapy significantly decreased tumor volume (P < 0.05), with recurrence in only 25% of animals tested, compared to 78% tumor regrowth after LITT alone. These results suggest that laser chemotherapy may be an effective treatment for head and neck SCCA.

AB - Recent studies using murine models of human squamous cell carcinoma (SCCA) have revealed a significant improvement in survival and cure rate of animals transplanted with human SCCA when treated with a combination of intratumor injections of chemotherapy and laser induced thermal therapy (LITT). These preliminary results suggest that this novel combination therapy may lead to improved clinical response compared to either treatment modality alone. Using a murine model of human SCCA we investigated two different modes of intratumor injection of cisplatin: a sustained-release cisplatin gel implant (CDDP/gel) versus cisplatin in solution (CDDP) at varying doses (range 1-3 mg/ml). In addition, we tested CDDP/gel combined with LITT. Results showed optimal drug concentration (30-300 nM) at tumor margins up to 4 h after injection of CDDP/gel implant compared to 3 nM at 5 min after injection with CDDP solution. Combined CDDP/gel and laser therapy significantly decreased tumor volume (P < 0.05), with recurrence in only 25% of animals tested, compared to 78% tumor regrowth after LITT alone. These results suggest that laser chemotherapy may be an effective treatment for head and neck SCCA.

KW - Biodistribution

KW - Cisplatin

KW - Intratumor-injections

KW - Laser-induced thermal therapy

KW - Squamous cell carcinoma

UR - http://www.scopus.com/inward/record.url?scp=57649148562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57649148562&partnerID=8YFLogxK

U2 - 10.1007/s00405-008-0736-3

DO - 10.1007/s00405-008-0736-3

M3 - Article

VL - 266

SP - 279

EP - 284

JO - European Archives of Oto-Rhino-Laryngology

JF - European Archives of Oto-Rhino-Laryngology

SN - 0937-4477

IS - 2

ER -