Resveratrol given intraperitoneally does not inhibit the growth of high-risk t(4;11) acute lymphoblastic leukemia cells in a NOD/SCID mouse model

Susan J. Zunino, David H. Storms, John W. Newman, Theresa L. Pedersen, Carl L Keen, Jonathan M Ducore

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The efficacy of resveratrol as a preventive agent against the growth of t(4;11) acute lymphoblastic leukemia (ALL) was evaluated in NOD.CB17-Prkdcscid/J mice engrafted with the human t(4;11) ALL SEM cell line. SEM cells were injected into the tail vein and engraftment was monitored by flow cytometry. Once engraftment was observed, mice were injected intraperitoneally with resveratrol (10 mg/kg body weight) dissolved in dimethylsulfoxide (DMSO) or DMSO alone (control) every other day, or vincristine (0.5 mg/kg body weight) 3 times per week for 4 weeks (n=16 per group). Comparisons of the percent of human leukemia cells in blood and survival curves showed resveratrol did not inhibit progression of the disease. Liquid chromatography-tandem mass spectrometry analyses of mouse sera showed resveratrol was rapidly metabolized to glucuronidated and sulfated forms 1 h post-injection, with low to no resveratrol or metabolites observed in sera by 24-48 h. These data indicate that in contrast to findings in in vitro models, parenterally administered resveratrol does not have potential as a preventive agent against high risk t(4;11) ALL.

Original languageEnglish (US)
Pages (from-to)1277-1284
Number of pages8
JournalInternational Journal of Oncology
Volume40
Issue number4
DOIs
StatePublished - Apr 2012

Keywords

  • Metabolites
  • NOD/SCID mice
  • Resveratrol
  • t(4;11) acute lymphoblastic leukemia
  • Vincristine

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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