Results of cytologic and microbiologic analysis of bronchoalveolar lavage fluid in New Zealand White rabbits

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Abstract

Objective - To determine cytologic and microbiologic findings in bronchoalveolar lavage (BAL) fluid and Spo2 values obtained during BAL in healthy rabbits. Animals - 9 rabbits. Procedures - Bronchoscopic BAL of left and right caudal lobar bronchi (LB2 and RB4) was performed with 3 mL of sterile saline (0.9% NaCl) solution; Spo2 was measured before, during, and after BAL. Percentage fluid recovered, total leukocyte counts, and differential cell counts were determined. Aerobic and anaerobic bacterial, mycoplasmal, and fungal cultures were performed from combined LB2 and RB4 samples. Results - Mean ± SD percentage fluid volumes recovered from LB2 and RB4 were 53 ± 13% and 63 ± 13%, respectively. Mean ± SD total leukocyte counts from LB2 and RB4 were 422 ± 199 cells/μL and 378 ± 97 cells/μL, respectively. Macrophages were most frequently identified. There were no significant differences in volumes retrieved, total leukocyte counts, or differential cell percentages between LB2 and RB4. Microbial culture results were negative for 3 rabbits and positive for mixed aerobic and anaerobic bacterial growth in 6 and 2 rabbits, respectively. The Spo2 was ≥ 95% in 7 of 9 rabbits after anesthetic induction, < 95% in 5 of 6 rabbits 1 minute after BAL, and ≥ 95% in 5 of 9 rabbits and > 90% in 4 of 9 rabbits 3 minutes after BAL. Conclusions and clinical relevance - Bronchoscopic BAL with 3 mL of saline solution provided adequate fluid recovery for microbiologic and cytologic examination from the caudal lung lobes. Transient low Spo2 was detected immediately after BAL.

Original languageEnglish (US)
Pages (from-to)572-578
Number of pages7
JournalAmerican Journal of Veterinary Research
Volume69
Issue number5
DOIs
StatePublished - May 2008

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New Zealand White rabbit
Bronchoalveolar Lavage Fluid
Bronchoalveolar Lavage
rabbits
Rabbits
Leukocyte Count
leukocyte count
sodium chloride
cells
bronchi
Bronchi
Sodium Chloride
anesthetics
Anesthetics
microbial growth
fluids
macrophages
Cell Count
lungs
Macrophages

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{189a327c4b9a4c7a8031a70ad97cf8af,
title = "Results of cytologic and microbiologic analysis of bronchoalveolar lavage fluid in New Zealand White rabbits",
abstract = "Objective - To determine cytologic and microbiologic findings in bronchoalveolar lavage (BAL) fluid and Spo2 values obtained during BAL in healthy rabbits. Animals - 9 rabbits. Procedures - Bronchoscopic BAL of left and right caudal lobar bronchi (LB2 and RB4) was performed with 3 mL of sterile saline (0.9{\%} NaCl) solution; Spo2 was measured before, during, and after BAL. Percentage fluid recovered, total leukocyte counts, and differential cell counts were determined. Aerobic and anaerobic bacterial, mycoplasmal, and fungal cultures were performed from combined LB2 and RB4 samples. Results - Mean ± SD percentage fluid volumes recovered from LB2 and RB4 were 53 ± 13{\%} and 63 ± 13{\%}, respectively. Mean ± SD total leukocyte counts from LB2 and RB4 were 422 ± 199 cells/μL and 378 ± 97 cells/μL, respectively. Macrophages were most frequently identified. There were no significant differences in volumes retrieved, total leukocyte counts, or differential cell percentages between LB2 and RB4. Microbial culture results were negative for 3 rabbits and positive for mixed aerobic and anaerobic bacterial growth in 6 and 2 rabbits, respectively. The Spo2 was ≥ 95{\%} in 7 of 9 rabbits after anesthetic induction, < 95{\%} in 5 of 6 rabbits 1 minute after BAL, and ≥ 95{\%} in 5 of 9 rabbits and > 90{\%} in 4 of 9 rabbits 3 minutes after BAL. Conclusions and clinical relevance - Bronchoscopic BAL with 3 mL of saline solution provided adequate fluid recovery for microbiologic and cytologic examination from the caudal lung lobes. Transient low Spo2 was detected immediately after BAL.",
author = "Michelle Hawkins and William Vernau and Drazenovich, {Tracy L.} and Griffey, {Stephen M} and Johnson, {Lynelle R}",
year = "2008",
month = "5",
doi = "10.2460/ajvr.69.5.572",
language = "English (US)",
volume = "69",
pages = "572--578",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "5",

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TY - JOUR

T1 - Results of cytologic and microbiologic analysis of bronchoalveolar lavage fluid in New Zealand White rabbits

AU - Hawkins, Michelle

AU - Vernau, William

AU - Drazenovich, Tracy L.

AU - Griffey, Stephen M

AU - Johnson, Lynelle R

PY - 2008/5

Y1 - 2008/5

N2 - Objective - To determine cytologic and microbiologic findings in bronchoalveolar lavage (BAL) fluid and Spo2 values obtained during BAL in healthy rabbits. Animals - 9 rabbits. Procedures - Bronchoscopic BAL of left and right caudal lobar bronchi (LB2 and RB4) was performed with 3 mL of sterile saline (0.9% NaCl) solution; Spo2 was measured before, during, and after BAL. Percentage fluid recovered, total leukocyte counts, and differential cell counts were determined. Aerobic and anaerobic bacterial, mycoplasmal, and fungal cultures were performed from combined LB2 and RB4 samples. Results - Mean ± SD percentage fluid volumes recovered from LB2 and RB4 were 53 ± 13% and 63 ± 13%, respectively. Mean ± SD total leukocyte counts from LB2 and RB4 were 422 ± 199 cells/μL and 378 ± 97 cells/μL, respectively. Macrophages were most frequently identified. There were no significant differences in volumes retrieved, total leukocyte counts, or differential cell percentages between LB2 and RB4. Microbial culture results were negative for 3 rabbits and positive for mixed aerobic and anaerobic bacterial growth in 6 and 2 rabbits, respectively. The Spo2 was ≥ 95% in 7 of 9 rabbits after anesthetic induction, < 95% in 5 of 6 rabbits 1 minute after BAL, and ≥ 95% in 5 of 9 rabbits and > 90% in 4 of 9 rabbits 3 minutes after BAL. Conclusions and clinical relevance - Bronchoscopic BAL with 3 mL of saline solution provided adequate fluid recovery for microbiologic and cytologic examination from the caudal lung lobes. Transient low Spo2 was detected immediately after BAL.

AB - Objective - To determine cytologic and microbiologic findings in bronchoalveolar lavage (BAL) fluid and Spo2 values obtained during BAL in healthy rabbits. Animals - 9 rabbits. Procedures - Bronchoscopic BAL of left and right caudal lobar bronchi (LB2 and RB4) was performed with 3 mL of sterile saline (0.9% NaCl) solution; Spo2 was measured before, during, and after BAL. Percentage fluid recovered, total leukocyte counts, and differential cell counts were determined. Aerobic and anaerobic bacterial, mycoplasmal, and fungal cultures were performed from combined LB2 and RB4 samples. Results - Mean ± SD percentage fluid volumes recovered from LB2 and RB4 were 53 ± 13% and 63 ± 13%, respectively. Mean ± SD total leukocyte counts from LB2 and RB4 were 422 ± 199 cells/μL and 378 ± 97 cells/μL, respectively. Macrophages were most frequently identified. There were no significant differences in volumes retrieved, total leukocyte counts, or differential cell percentages between LB2 and RB4. Microbial culture results were negative for 3 rabbits and positive for mixed aerobic and anaerobic bacterial growth in 6 and 2 rabbits, respectively. The Spo2 was ≥ 95% in 7 of 9 rabbits after anesthetic induction, < 95% in 5 of 6 rabbits 1 minute after BAL, and ≥ 95% in 5 of 9 rabbits and > 90% in 4 of 9 rabbits 3 minutes after BAL. Conclusions and clinical relevance - Bronchoscopic BAL with 3 mL of saline solution provided adequate fluid recovery for microbiologic and cytologic examination from the caudal lung lobes. Transient low Spo2 was detected immediately after BAL.

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