Restriction Endonuclease Studies of Hyperplastic Outgrowth Lines from BALB/cfC3H Mouse Hyperplastic Mammary Nodules

Robert Cardiff, Thomas G. Fanning, David W. Morris, Rhoda L. Ashley, Leslie J. Faulkin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The DNA's isolated from five mouse hyperplastic mammary gland outgrowth lines from BALB/cfC3H mice were digested with the restriction endonucleases Pstl BamHI, or EcoRl; electrophoresed; and analyzed by Southern blotting and autoradiography. Proviral DNA sequences from the acquired C3H mouse mammary tumor virus were detected in the DNA of all five lines, indicating that they were infected. The DNA of the five hyperplastic lines contained more EcoRI and BamHI mouse mammary tumor virus proviral DNA fragments than did DNA from normal organs, suggesting that the hyperplastic tissues were composed of more homogeneous cell populations than was lactating mammary gland. Each hyperplastic line had unique and reproducible BamHI and EcoRI restriction (integration) patterns which were stable over as many as seven transplant generations. Three sublines, which originated from the same hyperplastic alveolar nodule, had unique integration patterns but also shared several fragments. On the basis of these observations, we propose that mouse mammary “hyperpla-sias” are clonal dominant premalignant neoplasms.

Original languageEnglish (US)
Pages (from-to)3024-3029
Number of pages6
JournalCancer Research
Volume41
Issue number8
StatePublished - Aug 1 1981

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DNA Restriction Enzymes
Breast
DNA
Mouse mammary tumor virus
Human Mammary Glands
Deoxyribonuclease BamHI
Inbred C3H Mouse
Southern Blotting
Autoradiography
Transplants
Population
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Cardiff, R., Fanning, T. G., Morris, D. W., Ashley, R. L., & Faulkin, L. J. (1981). Restriction Endonuclease Studies of Hyperplastic Outgrowth Lines from BALB/cfC3H Mouse Hyperplastic Mammary Nodules. Cancer Research, 41(8), 3024-3029.

Restriction Endonuclease Studies of Hyperplastic Outgrowth Lines from BALB/cfC3H Mouse Hyperplastic Mammary Nodules. / Cardiff, Robert; Fanning, Thomas G.; Morris, David W.; Ashley, Rhoda L.; Faulkin, Leslie J.

In: Cancer Research, Vol. 41, No. 8, 01.08.1981, p. 3024-3029.

Research output: Contribution to journalArticle

Cardiff, R, Fanning, TG, Morris, DW, Ashley, RL & Faulkin, LJ 1981, 'Restriction Endonuclease Studies of Hyperplastic Outgrowth Lines from BALB/cfC3H Mouse Hyperplastic Mammary Nodules', Cancer Research, vol. 41, no. 8, pp. 3024-3029.
Cardiff, Robert ; Fanning, Thomas G. ; Morris, David W. ; Ashley, Rhoda L. ; Faulkin, Leslie J. / Restriction Endonuclease Studies of Hyperplastic Outgrowth Lines from BALB/cfC3H Mouse Hyperplastic Mammary Nodules. In: Cancer Research. 1981 ; Vol. 41, No. 8. pp. 3024-3029.
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