Objective: Assessment of agonist-mediated responses in intrapulmonary airways of the rat and their alteration with ADSS. Method: Responses in individual airway segments were determined as changes in the luminal area of slices from agarose-filled lungs at different airway levels by computerized videomicrometry using male Sprague-Dawley rats exposed to filtered air or ADSS (1 mg/mm total suspended particulate level). Results: Optimal conditions for media volume, spontaneous airway tone, duration of response, dose of agonist and cumulative agonist concentration both in the presence and absence of indomethacin were determined by preliminary experiments. Indomethacin was not used in the final experiment as there was no effect of spontaneous tone within the time-frame of the experiment. There was clear concentration- response in the percentage of area reduction with methacholine in slices obtained from both filtered air and ADSS exposed animals. Differences in the response of airways were also noted based on their anatomical location. For a given agonist concentration, proximal airways responded to a lesser extent than distal airways. There was no significant difference in the responsiveness of the two groups after exposure to 3 days of ADSS. Conclusion: Lung slices can be useful in determining changes in agonist-induced airway responsiveness following ADSS exposure based on the airway location and generation.
|Original language||English (US)|
|State||Published - 1997|
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology