Response of fission yeast to toxic cations involves cooperative action of the stress-activated protein kinase Spc1/Sty1 and the Hal4 protein kinase

Ling Yu Wang, Koichi Shimada, Masayo Morishita, Kazuhiro Shiozaki

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Stress-activated protein kinases (SAPKs), members of a mitogen-activated protein kinase (MAPK) subfamily, are highly conserved among eukaryotes. Studies of yeasts demonstrated that SAPKs play pivotal roles in survival responses to high osmolarity, oxidative stress, and heat shock. Here we report a novel physiological role of the fission yeast Spc1 SAPK in cellular resistance to certain cations, such as Na+, Li+, and Ca2+. Strains lacking Spc1 or its activator, Wis1 MAPK kinase, are hypersensitive to these cations. Spc1 positively regulates expression of sod2+ encoding a Na+/H+ antiporter through Atf1 and other transcription factors. In addition, we have identified a novel Spc1-interacting protein, Hal4, which is highly homologous to the budding yeast Sat4/Hal4 protein kinase. Like its budding yeast counterpart, the fission yeast Hal4 kinase is essential for cellular resistance to Na+, Li+, and Ca2+. The hal4-null phenotype is complemented by overexpression of the Trk1 potassium transporter or increased K+ in the growth medium, suggesting that Hal4 promotes K+ uptake, which consequently increases cellular resistance to other cations. Interestingly, the Spc1-Hal4 interaction appears to be required for cellular resistance to Ca2+ but not Na+ and Li+. We propose that Spc1 SAPK and Hal4 kinase cooperatively function to protect cells from the toxic cations.

Original languageEnglish (US)
Pages (from-to)3945-3955
Number of pages11
JournalMolecular and Cellular Biology
Volume25
Issue number10
DOIs
StatePublished - May 2005

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Schizosaccharomyces
Poisons
Heat-Shock Proteins
Protein Kinases
Cations
Saccharomycetales
Phosphotransferases
Sodium-Hydrogen Antiporter
Mitogen-Activated Protein Kinase Kinases
Mitogen-Activated Protein Kinases
Eukaryota
Osmolar Concentration
Shock
Potassium
Oxidative Stress
Transcription Factors
Hot Temperature
Yeasts
Phenotype
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Response of fission yeast to toxic cations involves cooperative action of the stress-activated protein kinase Spc1/Sty1 and the Hal4 protein kinase. / Wang, Ling Yu; Shimada, Koichi; Morishita, Masayo; Shiozaki, Kazuhiro.

In: Molecular and Cellular Biology, Vol. 25, No. 10, 05.2005, p. 3945-3955.

Research output: Contribution to journalArticle

Wang, Ling Yu ; Shimada, Koichi ; Morishita, Masayo ; Shiozaki, Kazuhiro. / Response of fission yeast to toxic cations involves cooperative action of the stress-activated protein kinase Spc1/Sty1 and the Hal4 protein kinase. In: Molecular and Cellular Biology. 2005 ; Vol. 25, No. 10. pp. 3945-3955.
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