Response of collateral-dependent myocardium to vasopressin release during prolonged intense exercise

J. D. Symons, J. C. Longhurst, Charles L Stebbins

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8 Scopus citations


We hypothesized that vasopressin concentrations during exercise attenuate the increase in collateral-dependent blood flow leading to left ventricular dysfunction in Ameroid-occluded miniswine. An Ameroid occluder was placed around the proximal left circumflex coronary artery (LCX) of 19 miniswine. Ten weeks later V1 receptor blockade with the use of [d(CH2)5 Tyr- (Me)]arginine vasopressin (10-12 μg/kg iv) increased resting transmural flow (radioactive microspheres) in the LCX region, indicating the presence of V1 receptors. Neither injection of lysine vasopressin (125 pmol/kg) after V1 receptor blockade nor injection of two specific V2 receptor agonists caused changes in mean arterial pressure, heart rate, or left anterior descending coronary arterial flow velocity, indicating that V2 receptors mediate no appreciable vasodilation in the swine coronary circulation. Next the ratio of collateral to noncollateral flow and regional systolic wall thickening (sonomicrometer dimension gauges) were measured at rest and after 20 min of prolonged, intense treadmill exercise (85-90% of heart rate reserve) in the presence and absence of V1 receptor antagonism. This degree of exertion increased plasma lysine vasopressin from 6.2 ± 1.0 at rest to 21.0 ± 7.0 pg/ml (P < 0.05) during the unblocked run. However, the decrease in transmural blood flow ratio (collateral to noncollateral flow) from rest was similar during exercise before and after V1 receptor blockade (0.78 ± 0.07 and 0.80 ± 0.05, respectively; P < 0.05 vs. rest). Likewise, percent systolic wall thickening in the collateral-dependent region decreased from rest to exercise in the absence and presence of V1 receptor antagonism (35.9 ± 4.5 and 39.5 ± 3.8%, respectively; P < 0.05 vs. rest). Because V1 receptor blockade caused a significant reduction in mean and diastolic pressure during treadmill running, six pigs performed the same two exercise protocols except that dextran was infused during the V1 receptor blockade run to match blood pressures to those obtained during the unblocked run. Similar transmural blood flow ratios and systolic wall thickening responses were still observed before and after blockade. We conclude that coronary vasodilatory metabolites released during prolonged, intense exercise predominate over the potential vasoconstrictor effects of vasopressin.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number5 33-5
StatePublished - 1993


  • collateral vessels
  • myocardial blood flow
  • myocardial ischemia

ASJC Scopus subject areas

  • Physiology


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