Respiratory bronchiolitis following long-term ozone exposure in bonnet monkeys

A morphometric study

L. E. Fujinaka, D. M. Hyde, Charles Plopper, W. S. Tyler, D. L. Dungworth, L. O. Lollini

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

To quantitate the response of respiratory bronchiolar (RB) epithelium and peribronchiolar connective tissue (PCT) to chronic exposure to high ambient levels of ozone, two groups of 8 adult male bonnet monkeys each were subjected 8 h daily for one year to 0.64 ppm (UV standard) ozone or filtered air, respectively. Blocks of tissue selected throughout the lung and from first generation RBs following airway microdissection had the following significant exposure-related changes: 57% greater volume of RB in the lung, 27% smaller diameter of RB lumen, 179% thicker media and intima of peribronchiolar arterioles, 61% thicker RB epithelium, and 77% thicker PCT. The increase in thickness of the RB wall resulted primarily from an 84% increase in PCT, with the remainder from the epithelium. Estimates of cellular numerical density showed an 81% increase in cuboidal bronchiolar cells and an 87% decrease in type 1 pneumocytes in the exposed group. Cell volumes from serial section reconstruction showed significantly larger cuboidal bronchiolar (79% ciliated (117% and type 2 (66% cells over controls. Significant PCT changes included more amorphous extracellular matrix (288% neutrophils (1523% and lymphocytes/plasma cells (307% The number of fibroblasts and the volume of extracellular fibers were larger than control values by 44% and 31% in the exposed group, but these changes were not statistically significant. Centriacinar changes due to exposure to long-term, high ambient ozone in bonnet monkeys results in narrowing of respiratory bronchioles primarily by peribronchiolar inflammation (inflammatory cells, fibers, amorphous matrix) and secondarily through hyperplasia of cuboidal bronchiolar cells.

Original languageEnglish (US)
Pages (from-to)167-190
Number of pages24
JournalExperimental Lung Research
Volume8
Issue number2-3
DOIs
StatePublished - 1985

Fingerprint

Macaca radiata
Bronchiolitis
Ozone
Connective Tissue
Tissue
Respiratory Mucosa
Alveolar Epithelial Cells
Bronchioles
Microdissection
Lung
Arterioles
Plasma Cells
Cell Size
Lymphocytes
Fibers
Hyperplasia
Extracellular Matrix
Fibroblasts
Neutrophils
Epithelium

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Respiratory bronchiolitis following long-term ozone exposure in bonnet monkeys : A morphometric study. / Fujinaka, L. E.; Hyde, D. M.; Plopper, Charles; Tyler, W. S.; Dungworth, D. L.; Lollini, L. O.

In: Experimental Lung Research, Vol. 8, No. 2-3, 1985, p. 167-190.

Research output: Contribution to journalArticle

Fujinaka, L. E. ; Hyde, D. M. ; Plopper, Charles ; Tyler, W. S. ; Dungworth, D. L. ; Lollini, L. O. / Respiratory bronchiolitis following long-term ozone exposure in bonnet monkeys : A morphometric study. In: Experimental Lung Research. 1985 ; Vol. 8, No. 2-3. pp. 167-190.
@article{aa1a0ce975994a48b8e0dbab6a9e74c3,
title = "Respiratory bronchiolitis following long-term ozone exposure in bonnet monkeys: A morphometric study",
abstract = "To quantitate the response of respiratory bronchiolar (RB) epithelium and peribronchiolar connective tissue (PCT) to chronic exposure to high ambient levels of ozone, two groups of 8 adult male bonnet monkeys each were subjected 8 h daily for one year to 0.64 ppm (UV standard) ozone or filtered air, respectively. Blocks of tissue selected throughout the lung and from first generation RBs following airway microdissection had the following significant exposure-related changes: 57{\%} greater volume of RB in the lung, 27{\%} smaller diameter of RB lumen, 179{\%} thicker media and intima of peribronchiolar arterioles, 61{\%} thicker RB epithelium, and 77{\%} thicker PCT. The increase in thickness of the RB wall resulted primarily from an 84{\%} increase in PCT, with the remainder from the epithelium. Estimates of cellular numerical density showed an 81{\%} increase in cuboidal bronchiolar cells and an 87{\%} decrease in type 1 pneumocytes in the exposed group. Cell volumes from serial section reconstruction showed significantly larger cuboidal bronchiolar (79{\%} ciliated (117{\%} and type 2 (66{\%} cells over controls. Significant PCT changes included more amorphous extracellular matrix (288{\%} neutrophils (1523{\%} and lymphocytes/plasma cells (307{\%} The number of fibroblasts and the volume of extracellular fibers were larger than control values by 44{\%} and 31{\%} in the exposed group, but these changes were not statistically significant. Centriacinar changes due to exposure to long-term, high ambient ozone in bonnet monkeys results in narrowing of respiratory bronchioles primarily by peribronchiolar inflammation (inflammatory cells, fibers, amorphous matrix) and secondarily through hyperplasia of cuboidal bronchiolar cells.",
author = "Fujinaka, {L. E.} and Hyde, {D. M.} and Charles Plopper and Tyler, {W. S.} and Dungworth, {D. L.} and Lollini, {L. O.}",
year = "1985",
doi = "10.3109/01902148509057520",
language = "English (US)",
volume = "8",
pages = "167--190",
journal = "Experimental Lung Research",
issn = "0190-2148",
publisher = "Informa Healthcare",
number = "2-3",

}

TY - JOUR

T1 - Respiratory bronchiolitis following long-term ozone exposure in bonnet monkeys

T2 - A morphometric study

AU - Fujinaka, L. E.

AU - Hyde, D. M.

AU - Plopper, Charles

AU - Tyler, W. S.

AU - Dungworth, D. L.

AU - Lollini, L. O.

PY - 1985

Y1 - 1985

N2 - To quantitate the response of respiratory bronchiolar (RB) epithelium and peribronchiolar connective tissue (PCT) to chronic exposure to high ambient levels of ozone, two groups of 8 adult male bonnet monkeys each were subjected 8 h daily for one year to 0.64 ppm (UV standard) ozone or filtered air, respectively. Blocks of tissue selected throughout the lung and from first generation RBs following airway microdissection had the following significant exposure-related changes: 57% greater volume of RB in the lung, 27% smaller diameter of RB lumen, 179% thicker media and intima of peribronchiolar arterioles, 61% thicker RB epithelium, and 77% thicker PCT. The increase in thickness of the RB wall resulted primarily from an 84% increase in PCT, with the remainder from the epithelium. Estimates of cellular numerical density showed an 81% increase in cuboidal bronchiolar cells and an 87% decrease in type 1 pneumocytes in the exposed group. Cell volumes from serial section reconstruction showed significantly larger cuboidal bronchiolar (79% ciliated (117% and type 2 (66% cells over controls. Significant PCT changes included more amorphous extracellular matrix (288% neutrophils (1523% and lymphocytes/plasma cells (307% The number of fibroblasts and the volume of extracellular fibers were larger than control values by 44% and 31% in the exposed group, but these changes were not statistically significant. Centriacinar changes due to exposure to long-term, high ambient ozone in bonnet monkeys results in narrowing of respiratory bronchioles primarily by peribronchiolar inflammation (inflammatory cells, fibers, amorphous matrix) and secondarily through hyperplasia of cuboidal bronchiolar cells.

AB - To quantitate the response of respiratory bronchiolar (RB) epithelium and peribronchiolar connective tissue (PCT) to chronic exposure to high ambient levels of ozone, two groups of 8 adult male bonnet monkeys each were subjected 8 h daily for one year to 0.64 ppm (UV standard) ozone or filtered air, respectively. Blocks of tissue selected throughout the lung and from first generation RBs following airway microdissection had the following significant exposure-related changes: 57% greater volume of RB in the lung, 27% smaller diameter of RB lumen, 179% thicker media and intima of peribronchiolar arterioles, 61% thicker RB epithelium, and 77% thicker PCT. The increase in thickness of the RB wall resulted primarily from an 84% increase in PCT, with the remainder from the epithelium. Estimates of cellular numerical density showed an 81% increase in cuboidal bronchiolar cells and an 87% decrease in type 1 pneumocytes in the exposed group. Cell volumes from serial section reconstruction showed significantly larger cuboidal bronchiolar (79% ciliated (117% and type 2 (66% cells over controls. Significant PCT changes included more amorphous extracellular matrix (288% neutrophils (1523% and lymphocytes/plasma cells (307% The number of fibroblasts and the volume of extracellular fibers were larger than control values by 44% and 31% in the exposed group, but these changes were not statistically significant. Centriacinar changes due to exposure to long-term, high ambient ozone in bonnet monkeys results in narrowing of respiratory bronchioles primarily by peribronchiolar inflammation (inflammatory cells, fibers, amorphous matrix) and secondarily through hyperplasia of cuboidal bronchiolar cells.

UR - http://www.scopus.com/inward/record.url?scp=0022416799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022416799&partnerID=8YFLogxK

U2 - 10.3109/01902148509057520

DO - 10.3109/01902148509057520

M3 - Article

VL - 8

SP - 167

EP - 190

JO - Experimental Lung Research

JF - Experimental Lung Research

SN - 0190-2148

IS - 2-3

ER -