Resistance to downy mildew in lettuce 'La Brillante' is conferred by Dm50 gene and multiple QTL

Ivan Simko, Oswaldo E. Ochoa, Mathieu A. Pel, Cayla Tsuchida, Carolina Font I Forcada, Ryan J. Hayes, Maria Jose Truco, Rudie Antonise, Carlos H. Galeano, Richard W Michelmore

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Many cultivars of lettuce (Lactuca sativa L.) are susceptible to downy mildew, a nearly globally ubiquitous disease caused by Bremia lactucae. We previously determined that Batavia type cultivar 'La Brillante' has a high level of field resistance to the disease in California. Testing of a mapping population developed from a cross between 'Salinas 88' and La Brillante in multiple field and laboratory experiments revealed that at least five loci conferred resistance in La Brillante. The presence of a new dominant resistance gene (designated Dm50) that confers complete resistance to specific isolates was detected in laboratory tests of seedlings inoculated with multiple diverse isolates. Dm50 is located in the major resistance cluster on linkage group 2 that contains at least eight major, dominant Dm genes conferring resistance to downy mildew. However, this Dm gene is ineffective against the isolates of B. lactucae prevalent in the field in California and the Netherlands. A quantitative trait locus (QTL) located at the Dm50 chromosomal region (qDM2.2) was detected, though, when the amount of disease was evaluated a month before plants reached harvest maturity. Four additional QTL for resistance to B. lactucae were identified on linkage groups 4 (qDM4.1 and qDM4.2), 7 (qDM7.1), and 9 (qDM9.2). The largest effect was associated with qDM7.1 (up to 32.9% of the total phenotypic variance) that determined resistance in multiple field experiments. Markers identified in the present study will facilitate introduction of these resistance loci into commercial cultivars of lettuce.

Original languageEnglish (US)
Pages (from-to)1220-1228
Number of pages9
JournalPhytopathology
Volume105
Issue number9
DOIs
StatePublished - Sep 1 2015

Fingerprint

Bremia lactucae
downy mildew
lettuce
quantitative trait loci
linkage groups
cultivars
loci
genes
dominant genes
Lactuca sativa
phenotypic variation
Netherlands
seedlings
testing

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Simko, I., Ochoa, O. E., Pel, M. A., Tsuchida, C., Font I Forcada, C., Hayes, R. J., ... Michelmore, R. W. (2015). Resistance to downy mildew in lettuce 'La Brillante' is conferred by Dm50 gene and multiple QTL. Phytopathology, 105(9), 1220-1228. https://doi.org/10.1094/PHYTO-02-15-0057-R

Resistance to downy mildew in lettuce 'La Brillante' is conferred by Dm50 gene and multiple QTL. / Simko, Ivan; Ochoa, Oswaldo E.; Pel, Mathieu A.; Tsuchida, Cayla; Font I Forcada, Carolina; Hayes, Ryan J.; Truco, Maria Jose; Antonise, Rudie; Galeano, Carlos H.; Michelmore, Richard W.

In: Phytopathology, Vol. 105, No. 9, 01.09.2015, p. 1220-1228.

Research output: Contribution to journalArticle

Simko, I, Ochoa, OE, Pel, MA, Tsuchida, C, Font I Forcada, C, Hayes, RJ, Truco, MJ, Antonise, R, Galeano, CH & Michelmore, RW 2015, 'Resistance to downy mildew in lettuce 'La Brillante' is conferred by Dm50 gene and multiple QTL', Phytopathology, vol. 105, no. 9, pp. 1220-1228. https://doi.org/10.1094/PHYTO-02-15-0057-R
Simko I, Ochoa OE, Pel MA, Tsuchida C, Font I Forcada C, Hayes RJ et al. Resistance to downy mildew in lettuce 'La Brillante' is conferred by Dm50 gene and multiple QTL. Phytopathology. 2015 Sep 1;105(9):1220-1228. https://doi.org/10.1094/PHYTO-02-15-0057-R
Simko, Ivan ; Ochoa, Oswaldo E. ; Pel, Mathieu A. ; Tsuchida, Cayla ; Font I Forcada, Carolina ; Hayes, Ryan J. ; Truco, Maria Jose ; Antonise, Rudie ; Galeano, Carlos H. ; Michelmore, Richard W. / Resistance to downy mildew in lettuce 'La Brillante' is conferred by Dm50 gene and multiple QTL. In: Phytopathology. 2015 ; Vol. 105, No. 9. pp. 1220-1228.
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AU - Font I Forcada, Carolina

AU - Hayes, Ryan J.

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