Resistance of colostrum-deprived domestic lambs to infection with deer adenovirus

Jennifer K. Imus, Howard D. Lehmkuhl, Leslie Woods

Research output: Contribution to journalArticle

Abstract

Seven colostrum-deprived, 3–4-wk-old Rambouillet–Hampshire lambs were inoculated via the mucous membranes with deer adenovirus (DAdV) and monitored for clinical signs for 21 d post-inoculation at which time animals were euthanized and postmortem examinations were performed. Pre-inoculation and post-inoculation serum samples were tested for antibodies to DAdV, ovine adenovirus 7, bovine adenovirus 7, and goat adenovirus 1. Evidence for DAdV infection was determined by virus isolation, PCR tests, and histopathology with immunohistochemistry tests for DAdV. No clinical signs or lesions consistent with adenoviral hemorrhagic disease (AHD) in deer were seen in the lambs, and the lambs did not seroconvert to DAdV. DAdV was not detected by PCR, virus isolation, or immunohistochemistry in any of the samples tested from the lambs. A positive control deer similarly inoculated with DAdV developed fatal AHD 1 wk post-inoculation. Our colostrum-deprived lambs did not become infected when inoculated with DAdV.

Original languageEnglish (US)
Pages (from-to)78-82
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume31
Issue number1
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

Atadenovirus
Colostrum
Adenoviridae
colostrum
deer
lambs
Infection
infection
Deer
immunohistochemistry
Immunohistochemistry
Viruses
Adenoviridae Infections
Polymerase Chain Reaction
viruses
Goats
lesions (animal)
histopathology
Autopsy
Mucous Membrane

Keywords

  • Deer adenovirus
  • Deer atadenovirus A experimental infection
  • sheep

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Resistance of colostrum-deprived domestic lambs to infection with deer adenovirus. / Imus, Jennifer K.; Lehmkuhl, Howard D.; Woods, Leslie.

In: Journal of Veterinary Diagnostic Investigation, Vol. 31, No. 1, 01.01.2019, p. 78-82.

Research output: Contribution to journalArticle

@article{c3f7ea9e1f5d498cb256d163b5f5c3d5,
title = "Resistance of colostrum-deprived domestic lambs to infection with deer adenovirus",
abstract = "Seven colostrum-deprived, 3–4-wk-old Rambouillet–Hampshire lambs were inoculated via the mucous membranes with deer adenovirus (DAdV) and monitored for clinical signs for 21 d post-inoculation at which time animals were euthanized and postmortem examinations were performed. Pre-inoculation and post-inoculation serum samples were tested for antibodies to DAdV, ovine adenovirus 7, bovine adenovirus 7, and goat adenovirus 1. Evidence for DAdV infection was determined by virus isolation, PCR tests, and histopathology with immunohistochemistry tests for DAdV. No clinical signs or lesions consistent with adenoviral hemorrhagic disease (AHD) in deer were seen in the lambs, and the lambs did not seroconvert to DAdV. DAdV was not detected by PCR, virus isolation, or immunohistochemistry in any of the samples tested from the lambs. A positive control deer similarly inoculated with DAdV developed fatal AHD 1 wk post-inoculation. Our colostrum-deprived lambs did not become infected when inoculated with DAdV.",
keywords = "Deer adenovirus, Deer atadenovirus A experimental infection, sheep",
author = "Imus, {Jennifer K.} and Lehmkuhl, {Howard D.} and Leslie Woods",
year = "2019",
month = "1",
day = "1",
doi = "10.1177/1040638718817508",
language = "English (US)",
volume = "31",
pages = "78--82",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
number = "1",

}

TY - JOUR

T1 - Resistance of colostrum-deprived domestic lambs to infection with deer adenovirus

AU - Imus, Jennifer K.

AU - Lehmkuhl, Howard D.

AU - Woods, Leslie

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Seven colostrum-deprived, 3–4-wk-old Rambouillet–Hampshire lambs were inoculated via the mucous membranes with deer adenovirus (DAdV) and monitored for clinical signs for 21 d post-inoculation at which time animals were euthanized and postmortem examinations were performed. Pre-inoculation and post-inoculation serum samples were tested for antibodies to DAdV, ovine adenovirus 7, bovine adenovirus 7, and goat adenovirus 1. Evidence for DAdV infection was determined by virus isolation, PCR tests, and histopathology with immunohistochemistry tests for DAdV. No clinical signs or lesions consistent with adenoviral hemorrhagic disease (AHD) in deer were seen in the lambs, and the lambs did not seroconvert to DAdV. DAdV was not detected by PCR, virus isolation, or immunohistochemistry in any of the samples tested from the lambs. A positive control deer similarly inoculated with DAdV developed fatal AHD 1 wk post-inoculation. Our colostrum-deprived lambs did not become infected when inoculated with DAdV.

AB - Seven colostrum-deprived, 3–4-wk-old Rambouillet–Hampshire lambs were inoculated via the mucous membranes with deer adenovirus (DAdV) and monitored for clinical signs for 21 d post-inoculation at which time animals were euthanized and postmortem examinations were performed. Pre-inoculation and post-inoculation serum samples were tested for antibodies to DAdV, ovine adenovirus 7, bovine adenovirus 7, and goat adenovirus 1. Evidence for DAdV infection was determined by virus isolation, PCR tests, and histopathology with immunohistochemistry tests for DAdV. No clinical signs or lesions consistent with adenoviral hemorrhagic disease (AHD) in deer were seen in the lambs, and the lambs did not seroconvert to DAdV. DAdV was not detected by PCR, virus isolation, or immunohistochemistry in any of the samples tested from the lambs. A positive control deer similarly inoculated with DAdV developed fatal AHD 1 wk post-inoculation. Our colostrum-deprived lambs did not become infected when inoculated with DAdV.

KW - Deer adenovirus

KW - Deer atadenovirus A experimental infection

KW - sheep

UR - http://www.scopus.com/inward/record.url?scp=85060060361&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060060361&partnerID=8YFLogxK

U2 - 10.1177/1040638718817508

DO - 10.1177/1040638718817508

M3 - Article

VL - 31

SP - 78

EP - 82

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 1

ER -