Requirements for Neurogenin2 during mouse postnatal retinal neurogenesis

Angelica M. Kowalchuk, Kate A. Maurer, Farnaz Shoja-Taheri, Nadean L Brown

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

During embryonic retinal development, the bHLH factor Neurog2 regulates the temporal progression of neurogenesis, but no role has been assigned for this gene in the postnatal retina. Using Neurog2 conditional mutants, we found that Neurog2 is necessary for the development of an early, embryonic cohort of rod photoreceptors, but also required by both a subset of cone bipolar subtypes, and rod bipolars. Using transcriptomics, we identified a subset of downregulated genes in P2 Neurog2 mutants, which act during rod differentiation, outer segment morphogenesis or visual processing. We also uncovered defects in neuronal cell culling, which suggests that the rod and bipolar cell phenotypes may arise via more complex mechanisms rather than a simple cell fate shift. However, given an overall phenotypic resemblance between Neurog2 and Blimp1 mutants, we explored the relationship between these two factors. We found that Blimp1 is downregulated between E12-birth in Neurog2 mutants, which probably reflects a dependence on Neurog2 in embryonic progenitor cells. Overall, we conclude that the Neurog2 gene is expressed and active prior to birth, but also exerts an influence on postnatal retinal neuron differentiation.

Original languageEnglish (US)
JournalDevelopmental Biology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Neurogenesis
Embryonic Development
Down-Regulation
Parturition
Genes
Retinal Neurons
Rod Cell Outer Segment
Retinal Rod Photoreceptor Cells
Morphogenesis
Retina
Stem Cells
Phenotype

Keywords

  • Bipolar cell
  • Blimp1/Prdm1
  • Neurog2
  • Retina
  • Rod photoreceptor

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Requirements for Neurogenin2 during mouse postnatal retinal neurogenesis. / Kowalchuk, Angelica M.; Maurer, Kate A.; Shoja-Taheri, Farnaz; Brown, Nadean L.

In: Developmental Biology, 01.01.2018.

Research output: Contribution to journalArticle

Kowalchuk, Angelica M. ; Maurer, Kate A. ; Shoja-Taheri, Farnaz ; Brown, Nadean L. / Requirements for Neurogenin2 during mouse postnatal retinal neurogenesis. In: Developmental Biology. 2018.
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