Requirements for Jag1-Rbpj mediated Notch signaling during early mouse lens development

Tien T. Le, Kevin W. Conley, Timothy J. Mead, Sheldon Rowan, Katherine E. Yutzey, Nadean L Brown

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Background: During vertebrate lens development, the lens placode in the embryonic ectoderm invaginates into a lens vesicle, which then separates from the surface epithelium, followed by two waves of fiber cell differentiation. In the mouse, multiple labs have shown that Jag1-Notch signaling is critically required during the second wave of lens fiber cell formation. However, Notch signaling appears to play no obvious role during lens induction or morphogenesis, although multiple pathway genes are expressed at these earlier stages. Results: Here, we explored functions for Notch signaling specifically during early lens development, by using the early-acting AP2α-Cre driver to delete Jag1 or Rbpj. We found that Jag1 and Rbpj are not required during lens induction, but are necessary for proper lens vesicle separation from the surface ectoderm. Conclusions: We conclude that precise levels of Notch signaling are essential during lens vesicle morphogenesis. In addition, AP2α-Cre-mediated deletion of Rbpj resulted in embryos with cardiac outflow tract and liver deformities, and perinatal lethality.

Original languageEnglish (US)
Pages (from-to)493-504
Number of pages12
JournalDevelopmental Dynamics
Issue number3
StatePublished - Mar 2012
Externally publishedYes


  • Apoptosis
  • Cardiac outflow tract
  • Jagged1
  • Lens development
  • Lens fiber cells
  • Liver development
  • Notch signaling
  • Rbpj

ASJC Scopus subject areas

  • Developmental Biology


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