Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets

John Ellis, Steven Krakowka, Michael Dale Lairmore, Deborah Haines, Ana Bratanich, Edward Clark, Gordon Allan, Carrie Konoby, Lori Hassard, Brian Meehan, Karen Martin, John Harding, Seamus Kennedy, Francis McNeilly

Research output: Contribution to journalArticle

227 Citations (Scopus)

Abstract

Neonatal gnotobiotic piglets were inoculated with tissue homogenates and low- and high-passage cell culture material to determine if the lesions of the newly described porcine postweaning multisystemic wasting syndrome (PMWS) could be reproduced. For this, 17 3-day-old gnotobiotic piglets were inoculated intranasally with pelleted chloroform-treated, filtered extracts from cell cultures, filter-sterilized homogenates of lymphoid tissue from PMWS-affected piglets, or control materials. Piglets were maintained in germ-free isolators for up to 5 weeks after infection prior to euthanasia and collection of samples for analysis. All piglets inoculated with the viral inocula developed lesions typical of PMWS, including generalized lymphadenopathy, hepatitis, nephritis, interstitial pneumonia, myocarditis, and gastritis. Porcine circovirus (PCV), as well as porcine parvovirus (PPV), was detected in tissues by virus reisolation, polymerase chain reaction analysis, or immunohistochemistry. All infected piglets developed moderate to high titers of antibody to PCV and moderate titers to PPV. No lesions, virus, or virus-specific antibodies were detected in sham-inoculated or uninoculated control piglets. These studies demonstrate that the lesions of PMWS can be experimentally reproduced in gnotobiotic piglets using filterable viral agents derived from pigs with PMWS and provide an experimental basis for further investigation into the pathogenesis and control of this emerging infectious disease in swine.

Original languageEnglish (US)
Pages (from-to)3-14
Number of pages12
JournalJournal of Veterinary Diagnostic Investigation
Volume11
Issue number1
StatePublished - Jan 1999
Externally publishedYes

Fingerprint

Germ-Free Life
Wasting Syndrome
postweaning multisystemic wasting syndrome
lesions (animal)
Reproduction
piglets
Porcine Parvovirus
Circovirus
Viruses
Porcine Postweaning Multisystemic Wasting Syndrome
Swine
Cell Culture Techniques
Porcine circovirus
Emerging Communicable Diseases
Ungulate protoparvovirus 1
Euthanasia
Antibodies
Nephritis
Interstitial Lung Diseases
Myocarditis

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Ellis, J., Krakowka, S., Lairmore, M. D., Haines, D., Bratanich, A., Clark, E., ... McNeilly, F. (1999). Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. Journal of Veterinary Diagnostic Investigation, 11(1), 3-14.

Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. / Ellis, John; Krakowka, Steven; Lairmore, Michael Dale; Haines, Deborah; Bratanich, Ana; Clark, Edward; Allan, Gordon; Konoby, Carrie; Hassard, Lori; Meehan, Brian; Martin, Karen; Harding, John; Kennedy, Seamus; McNeilly, Francis.

In: Journal of Veterinary Diagnostic Investigation, Vol. 11, No. 1, 01.1999, p. 3-14.

Research output: Contribution to journalArticle

Ellis, J, Krakowka, S, Lairmore, MD, Haines, D, Bratanich, A, Clark, E, Allan, G, Konoby, C, Hassard, L, Meehan, B, Martin, K, Harding, J, Kennedy, S & McNeilly, F 1999, 'Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets', Journal of Veterinary Diagnostic Investigation, vol. 11, no. 1, pp. 3-14.
Ellis, John ; Krakowka, Steven ; Lairmore, Michael Dale ; Haines, Deborah ; Bratanich, Ana ; Clark, Edward ; Allan, Gordon ; Konoby, Carrie ; Hassard, Lori ; Meehan, Brian ; Martin, Karen ; Harding, John ; Kennedy, Seamus ; McNeilly, Francis. / Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. In: Journal of Veterinary Diagnostic Investigation. 1999 ; Vol. 11, No. 1. pp. 3-14.
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