Reporter gene imaging using radiographic contrast from nonradioactive iodide sequestered by the sodium-iodide symporter

Stephen L. Brown, Svend O. Freytag, Kenneth N. Barton, Michael J. Flynn, Donald J. Peck, Aleksandar F. Dragovic, Ryan Jin, Yener N. Yeni, David P Fyhrie, Clifford M. Les, Guopei Zhu, Andrew Kolozsvary, Wayne C. Pitchford, S. David Nathanson, Joseph D. Fenstermacher, Jae Ho Kim

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The hypothesis that the human sodium-iodide symporter, NIS, can be used to detect NIS expression using standard radiological techniques was tested using adenoviral transduced NIS expression in human tumor xenografts grown in mice and in a naive dog prostate. Nonradioactive iodide was administered systemically to animals that 1-3 days previously had received a local injection of a replication-competent adenovirus expressing NIS under the control of the CMV promoter. The distribution of radiopacity was assessed in mouse tumors using micro-CT and a clinical X-ray machine and in the prostate of an anesthetized dog using a clinical spiral CT. Iodide sequestration and NIS expression were measured using X-ray spectrochemical analysis and fluorescence microscopy, respectively. Radiographic contrast due to NIS gene expression that was observed indicates the technique has potential for use in preclinical rodent tumor studies but probably lacks sensitivity for human use.

Original languageEnglish (US)
Pages (from-to)240-247
Number of pages8
JournalContrast Media and Molecular Imaging
Volume2
Issue number5
DOIs
StatePublished - Sep 2007
Externally publishedYes

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Keywords

  • Nonradioactive radiographic contrast
  • Reporter gene imaging
  • Sodium-iodide symporter

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Brown, S. L., Freytag, S. O., Barton, K. N., Flynn, M. J., Peck, D. J., Dragovic, A. F., Jin, R., Yeni, Y. N., Fyhrie, D. P., Les, C. M., Zhu, G., Kolozsvary, A., Pitchford, W. C., Nathanson, S. D., Fenstermacher, J. D., & Kim, J. H. (2007). Reporter gene imaging using radiographic contrast from nonradioactive iodide sequestered by the sodium-iodide symporter. Contrast Media and Molecular Imaging, 2(5), 240-247. https://doi.org/10.1002/cmmi.150