Report of wood decay fungus Inonotus tropicalis (phylum Basidiomycota) from a dog with a granulomatous mediastinal mass

Barbara J. Sheppard, Elizabeth McGrath, Michelle Giuffrida, Serena L.M. Craft, Chung Yee Kung, Matthew E. Smith

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A 75.9-kg, 3.5-year-old male Irish Wolfhound dog with a 2-3-week history of gagging and eating difficulties was referred to the University of Florida Veterinary Medical Hospital (Gainesville, Florida) for evaluation of a large cranial mediastinal mass suspected to be a thymoma or lymphosarcoma. The patient had 4 months of nearly 10 kg progressive weight loss with severe flank sensitivity and radiographically apparent lumbar vertebral changes interpreted as discospondylitis. Lab work revealed hyperglobulinemia, mild proteinuria, normal T4, negative Brucella canis titer, and negative blood and urine bacterial cultures. A thoracotomy revealed a nonresectable, destructive, space-occupying mediastinal mass resulting in euthanasia without surgical recovery. Biopsies from the mass were collected during surgery for histology. Microscopic examination revealed extensive granulomatous cellulitis and lymphadenitis characterized by central cavitated necrotic areas containing debris and degenerate neutrophils, intermediate zones of fibrovascular proliferation with marked mixed inflammation, peripheral fibrosis, frequent multinucleated macrophages, and scattered mineralization. The necrotic material contained dense mats of 2 μm wide by 8-15 μm long fungal hyphae with parallel walls, acute angle branching, frequent septae, and occasional bulb-like dilations. DNA sequencing and phylogenetic analysis of the internal transcribed spacer region confirmed the presence of a fungus in the Inonotus tropicalis group. Inonotus tropicalis is primarily a wood decay fungus that is found on dead wood from angiosperms in tropical and subtropical habitats. Isolates of the I. tropicalis group have been detected a few times from immunosuppressed human beings with X-linked granulomatous disease.

Original languageEnglish (US)
Pages (from-to)566-572
Number of pages7
JournalJournal of Veterinary Diagnostic Investigation
Volume25
Issue number5
DOIs
StatePublished - Sep 1 2013
Externally publishedYes

Fingerprint

Inonotus
decay fungi
Basidiomycota
decayed wood
Brucella canis
Fungi
Gagging
Dogs
Animal Hospitals
Angiosperms
Lymphadenitis
Cellulitis
Thymoma
Euthanasia
Hyphae
dogs
Thoracotomy
DNA Sequence Analysis
Proteinuria
Brucella melitensis biovar Canis

Keywords

  • Basidiomycota
  • granulomatous
  • Inonotus
  • Irish Wolfhounds
  • Phellinus

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Report of wood decay fungus Inonotus tropicalis (phylum Basidiomycota) from a dog with a granulomatous mediastinal mass. / Sheppard, Barbara J.; McGrath, Elizabeth; Giuffrida, Michelle; Craft, Serena L.M.; Kung, Chung Yee; Smith, Matthew E.

In: Journal of Veterinary Diagnostic Investigation, Vol. 25, No. 5, 01.09.2013, p. 566-572.

Research output: Contribution to journalArticle

Sheppard, Barbara J. ; McGrath, Elizabeth ; Giuffrida, Michelle ; Craft, Serena L.M. ; Kung, Chung Yee ; Smith, Matthew E. / Report of wood decay fungus Inonotus tropicalis (phylum Basidiomycota) from a dog with a granulomatous mediastinal mass. In: Journal of Veterinary Diagnostic Investigation. 2013 ; Vol. 25, No. 5. pp. 566-572.
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