Repeated stress induces dendritic spine loss in the rat medial prefrontal cortex

Jason J. Radley, Anne B. Rocher, Melinda Miller, William G M Janssen, Conor Liston, Patrick R. Hof, Bruce S. McEwen, John Morrison

Research output: Contribution to journalArticle

478 Citations (Scopus)

Abstract

The prefrontal cortex (PFC) plays an important role in higher cognitive processes, and in the regulation of stress-induced hypothalamic-pituitary- adrenal (HPA) activity. Here we examined the effect of repeated restraint stress on dendritic spine number in the medial PFC. Rats were perfused after receiving 21 days of daily restraint stress, and intracellular iontophoretic injections of Lucifer Yellow were carried out in layer II/III pyramidal neurons in the anterior cingulate and prelimbic cortices. We found that stress results in a significant (16%) decrease in apical dendritic spine density in medial PFC pyramidal neurons, and confirmed a previous observation that total apical dendritic length is reduced by 20% in the same neurons. We estimate that nearly one-third of all axospinous synapses on apical dendrites of pyramidal neurons in medial PFC are lost following repeated stress. A decrease in medial PFC dendritic spines may not only be indicative of a decrease in the total population of axospinous synapses, but may impair these neurons' capacity for biochemical compartmentalization and plasticity in which dendritic spines play a major role. Dendritic atrophy and spine loss may be important cellular features of stress-related psychiatric disorders where the PFC is functionally impaired.

Original languageEnglish (US)
Pages (from-to)313-320
Number of pages8
JournalCerebral Cortex
Volume16
Issue number3
DOIs
StatePublished - Mar 1 2006
Externally publishedYes

Fingerprint

Dendritic Spines
Prefrontal Cortex
Pyramidal Cells
Synapses
Neurons
Gyrus Cinguli
Dendrites
Atrophy
Psychiatry
Observation
Injections
Population

Keywords

  • Axospinous synapse
  • Cell loading
  • Dendritic spine
  • Post-traumatic stress disorder
  • Prefrontal
  • Stress

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Radley, J. J., Rocher, A. B., Miller, M., Janssen, W. G. M., Liston, C., Hof, P. R., ... Morrison, J. (2006). Repeated stress induces dendritic spine loss in the rat medial prefrontal cortex. Cerebral Cortex, 16(3), 313-320. https://doi.org/10.1093/cercor/bhi104

Repeated stress induces dendritic spine loss in the rat medial prefrontal cortex. / Radley, Jason J.; Rocher, Anne B.; Miller, Melinda; Janssen, William G M; Liston, Conor; Hof, Patrick R.; McEwen, Bruce S.; Morrison, John.

In: Cerebral Cortex, Vol. 16, No. 3, 01.03.2006, p. 313-320.

Research output: Contribution to journalArticle

Radley, JJ, Rocher, AB, Miller, M, Janssen, WGM, Liston, C, Hof, PR, McEwen, BS & Morrison, J 2006, 'Repeated stress induces dendritic spine loss in the rat medial prefrontal cortex', Cerebral Cortex, vol. 16, no. 3, pp. 313-320. https://doi.org/10.1093/cercor/bhi104
Radley JJ, Rocher AB, Miller M, Janssen WGM, Liston C, Hof PR et al. Repeated stress induces dendritic spine loss in the rat medial prefrontal cortex. Cerebral Cortex. 2006 Mar 1;16(3):313-320. https://doi.org/10.1093/cercor/bhi104
Radley, Jason J. ; Rocher, Anne B. ; Miller, Melinda ; Janssen, William G M ; Liston, Conor ; Hof, Patrick R. ; McEwen, Bruce S. ; Morrison, John. / Repeated stress induces dendritic spine loss in the rat medial prefrontal cortex. In: Cerebral Cortex. 2006 ; Vol. 16, No. 3. pp. 313-320.
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