TY - JOUR
T1 - Repeatability of vascular density measurement of the three retinal plexus layers using oct angiography in pathologic eyes (Octa vascular density repeatability of three plexus layers)
AU - Mukkamala, Lekha
AU - Nguyen, Michael
AU - Chang, Melinda
AU - Park, Susanna S.
N1 - Funding Information:
The research was supported in part by the University of California Davis Eye Center Kohl Medical Student Scholarship (MN). The authors would like to thank the retinal imaging team at the University of California Davis Eye Center for data collection. This was an investigator-initiated study without external financial support. However , we thank Optovue for providing the OCT/OCT A instrument and software as a loan for the study duration. Previous presentations Presented in part as a poster at the annual meeting of the American Society of Retinal Specialists, July 22, 2018, V ancouver , Canada and as a paper presentation at the UC Davis Annual Research Symposium, June 22, 2019, Sacramento, CA and at the UC Davis Kohl Medical Scholar Research Symposium, September 29, 2020.
Funding Information:
Susanna S Park reports research grant support via employer (Novartis/Roche; Allergan; Greybug) investigating anti-VEGF therapy for retinal disorders. The authors report no other potential conflicts of interest for this work.
PY - 2021
Y1 - 2021
N2 - Purpose: Although commercial optical coherence tomography angiography (OCTA) machines quantitate retinal vascular density (VD) by dividing the vasculature into superficial and deep capillary plexus (SCP, DCP), histology reveals three distinct plexus layers. This study tested the hypothesis that the VD measurement of three distinct retinal plexus layers obtained using custom segmentation has high repeatability comparable to that of automatically segmented SCP and DCP layers. Materials and Methods: Forty-four participants (86 eyes) were enrolled – 54 eyes with retinal vasculopathy and 25 eyes with macular edema. Macular OCTA images (3x3 mm and 6x6 mm) were obtained twice within 30 minutes by the same personnel using the same instrument (AngioVue, Optovue, version 2018.0.0.18). The intraclass correlation coefficient (ICC) was calculated to access repeatability. Results: The repeatability of VD for SCP and DCP was good-to-moderate (ICC=0.65–0.85) and minimally affected by image quality, retinal vasculopathy, or macular edema. The repeatability of the VD of the custom-segmented intermediate and deep plexus layers (cICP and cDCP) was poor/moderate (ICC=0.40–0.74) but better in the subset without macular edema using 3x3 mm scans with good images quality (ICC=0.58–0.93). Repeatability of cICP and cDCP VD measurement for 6x6 mm scans was poor (ICC≤0.5) in eyes with retinal vasculopathy and/or macular edema. Conclusion: Although repeatability of the VD measurement is high for the automatically segmented SCP and DCP, repeatability of VD is poor for the cICP and cDCP using larger scans in eyes with retinal vasculopathy and/or macular edema.
AB - Purpose: Although commercial optical coherence tomography angiography (OCTA) machines quantitate retinal vascular density (VD) by dividing the vasculature into superficial and deep capillary plexus (SCP, DCP), histology reveals three distinct plexus layers. This study tested the hypothesis that the VD measurement of three distinct retinal plexus layers obtained using custom segmentation has high repeatability comparable to that of automatically segmented SCP and DCP layers. Materials and Methods: Forty-four participants (86 eyes) were enrolled – 54 eyes with retinal vasculopathy and 25 eyes with macular edema. Macular OCTA images (3x3 mm and 6x6 mm) were obtained twice within 30 minutes by the same personnel using the same instrument (AngioVue, Optovue, version 2018.0.0.18). The intraclass correlation coefficient (ICC) was calculated to access repeatability. Results: The repeatability of VD for SCP and DCP was good-to-moderate (ICC=0.65–0.85) and minimally affected by image quality, retinal vasculopathy, or macular edema. The repeatability of the VD of the custom-segmented intermediate and deep plexus layers (cICP and cDCP) was poor/moderate (ICC=0.40–0.74) but better in the subset without macular edema using 3x3 mm scans with good images quality (ICC=0.58–0.93). Repeatability of cICP and cDCP VD measurement for 6x6 mm scans was poor (ICC≤0.5) in eyes with retinal vasculopathy and/or macular edema. Conclusion: Although repeatability of the VD measurement is high for the automatically segmented SCP and DCP, repeatability of VD is poor for the cICP and cDCP using larger scans in eyes with retinal vasculopathy and/or macular edema.
KW - Deep retinal plexus
KW - Intermediate retinal plexus
KW - Macular edema
KW - Middle retinal plexus
KW - Retinal vasculopathy
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U2 - 10.2147/OPTH.S284872
DO - 10.2147/OPTH.S284872
M3 - Article
AN - SCOPUS:85099907653
VL - 15
SP - 93
EP - 103
JO - Clinical Ophthalmology
JF - Clinical Ophthalmology
SN - 1177-5467
ER -