Abstract
Chinese hamster ovary cells and two UV-hypersensitive derivatives were used to determine the importance of DNA excision repair for split-dose recovery. In the wild-type cells 75% of the maximum theoretical recovery was observed when the fractions were delivered at 2-h intervals. Very little recovery was evident in the two hypersensitive cell lines. Using radioimmunoassays specific for (6-4)photoproducts and cyclobutane dimers, the ability of UV-irradiated repair-deficient cells representing 5 complementation groups to repair these 2 photoproducts was determined. Removal of antibody-binding sites specific for (6-4)photoproducts was 80% complete in 6 h and was defectiev in the UV-sensitive cells. In contrast, only 20--60% of antibody-binding sites specific for cylcobutane dimers were removed 18 h post-irradiation, and the extent of removal was the same in normal and defective cell lines. We conclude that repair pf (6-4)photoproducts accounts for split-dose recovery. In addition, we conclude thtat a consequences of DNA repair in CHO cells is modification rather that reemoval of cylobutane dimers.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 53-63 |
| Number of pages | 11 |
| Journal | Mutation Research DNA Repair Reports |
| Volume | 193 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1988 |
| Externally published | Yes |
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Keywords
- (6-4)Photoproducts
- Cyclobutane dimers
- DNA excision repair
- Split-dose recovery
ASJC Scopus subject areas
- Medicine(all)
Cite this
Repair of (6-4)photoproducts correlates with split-dose recovery in UV-irradiated normal and hypersensitive rodent cells. / Mitchell, David L.; Humphrey, Ronald M.; Adair, Gerald M.; Thompson, Larry H.; Clarkson, Judith M.
In: Mutation Research DNA Repair Reports, Vol. 193, No. 1, 1988, p. 53-63.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Repair of (6-4)photoproducts correlates with split-dose recovery in UV-irradiated normal and hypersensitive rodent cells
AU - Mitchell, David L.
AU - Humphrey, Ronald M.
AU - Adair, Gerald M.
AU - Thompson, Larry H.
AU - Clarkson, Judith M.
PY - 1988
Y1 - 1988
N2 - Chinese hamster ovary cells and two UV-hypersensitive derivatives were used to determine the importance of DNA excision repair for split-dose recovery. In the wild-type cells 75% of the maximum theoretical recovery was observed when the fractions were delivered at 2-h intervals. Very little recovery was evident in the two hypersensitive cell lines. Using radioimmunoassays specific for (6-4)photoproducts and cyclobutane dimers, the ability of UV-irradiated repair-deficient cells representing 5 complementation groups to repair these 2 photoproducts was determined. Removal of antibody-binding sites specific for (6-4)photoproducts was 80% complete in 6 h and was defectiev in the UV-sensitive cells. In contrast, only 20--60% of antibody-binding sites specific for cylcobutane dimers were removed 18 h post-irradiation, and the extent of removal was the same in normal and defective cell lines. We conclude that repair pf (6-4)photoproducts accounts for split-dose recovery. In addition, we conclude thtat a consequences of DNA repair in CHO cells is modification rather that reemoval of cylobutane dimers.
AB - Chinese hamster ovary cells and two UV-hypersensitive derivatives were used to determine the importance of DNA excision repair for split-dose recovery. In the wild-type cells 75% of the maximum theoretical recovery was observed when the fractions were delivered at 2-h intervals. Very little recovery was evident in the two hypersensitive cell lines. Using radioimmunoassays specific for (6-4)photoproducts and cyclobutane dimers, the ability of UV-irradiated repair-deficient cells representing 5 complementation groups to repair these 2 photoproducts was determined. Removal of antibody-binding sites specific for (6-4)photoproducts was 80% complete in 6 h and was defectiev in the UV-sensitive cells. In contrast, only 20--60% of antibody-binding sites specific for cylcobutane dimers were removed 18 h post-irradiation, and the extent of removal was the same in normal and defective cell lines. We conclude that repair pf (6-4)photoproducts accounts for split-dose recovery. In addition, we conclude thtat a consequences of DNA repair in CHO cells is modification rather that reemoval of cylobutane dimers.
KW - (6-4)Photoproducts
KW - Cyclobutane dimers
KW - DNA excision repair
KW - Split-dose recovery
UR - http://www.scopus.com/inward/record.url?scp=0023882323&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023882323&partnerID=8YFLogxK
U2 - 10.1016/0167-8817(88)90007-7
DO - 10.1016/0167-8817(88)90007-7
M3 - Article
VL - 193
SP - 53
EP - 63
JO - Mutation Research DNA Repair Reports
JF - Mutation Research DNA Repair Reports
SN - 0167-8817
IS - 1
ER -