Repair of (6-4)photoproducts correlates with split-dose recovery in UV-irradiated normal and hypersensitive rodent cells

David L. Mitchell; Ronald M. Humphrey; Gerald M. Adair; Larry H. Thompson; Judith M. Clarkson

doi:10.1016/0167-8817(88)90007-7

Repair of (6-4)photoproducts correlates with split-dose recovery in UV-irradiated normal and hypersensitive rodent cells

David L. Mitchell, Ronald M. Humphrey, Gerald M. Adair, Larry H. Thompson, Judith M. Clarkson

Research output: Contribution to journal › Article

44 Scopus citations

Abstract

Chinese hamster ovary cells and two UV-hypersensitive derivatives were used to determine the importance of DNA excision repair for split-dose recovery. In the wild-type cells 75% of the maximum theoretical recovery was observed when the fractions were delivered at 2-h intervals. Very little recovery was evident in the two hypersensitive cell lines. Using radioimmunoassays specific for (6-4)photoproducts and cyclobutane dimers, the ability of UV-irradiated repair-deficient cells representing 5 complementation groups to repair these 2 photoproducts was determined. Removal of antibody-binding sites specific for (6-4)photoproducts was 80% complete in 6 h and was defectiev in the UV-sensitive cells. In contrast, only 20--60% of antibody-binding sites specific for cylcobutane dimers were removed 18 h post-irradiation, and the extent of removal was the same in normal and defective cell lines. We conclude that repair pf (6-4)photoproducts accounts for split-dose recovery. In addition, we conclude thtat a consequences of DNA repair in CHO cells is modification rather that reemoval of cylobutane dimers.

Original language	English (US)
Pages (from-to)	53-63
Number of pages	11
Journal	Mutation Research DNA Repair Reports
Volume	193
Issue number	1
DOIs	https://doi.org/10.1016/0167-8817(88)90007-7
State	Published - 1988
Externally published	Yes

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Keywords

(6-4)Photoproducts
Cyclobutane dimers
DNA excision repair
Split-dose recovery

ASJC Scopus subject areas

Medicine(all)

Cite this

Mitchell, D. L., Humphrey, R. M., Adair, G. M., Thompson, L. H., & Clarkson, J. M. (1988). Repair of (6-4)photoproducts correlates with split-dose recovery in UV-irradiated normal and hypersensitive rodent cells. Mutation Research DNA Repair Reports, 193(1), 53-63. https://doi.org/10.1016/0167-8817(88)90007-7

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abstract = "Chinese hamster ovary cells and two UV-hypersensitive derivatives were used to determine the importance of DNA excision repair for split-dose recovery. In the wild-type cells 75% of the maximum theoretical recovery was observed when the fractions were delivered at 2-h intervals. Very little recovery was evident in the two hypersensitive cell lines. Using radioimmunoassays specific for (6-4)photoproducts and cyclobutane dimers, the ability of UV-irradiated repair-deficient cells representing 5 complementation groups to repair these 2 photoproducts was determined. Removal of antibody-binding sites specific for (6-4)photoproducts was 80% complete in 6 h and was defectiev in the UV-sensitive cells. In contrast, only 20--60% of antibody-binding sites specific for cylcobutane dimers were removed 18 h post-irradiation, and the extent of removal was the same in normal and defective cell lines. We conclude that repair pf (6-4)photoproducts accounts for split-dose recovery. In addition, we conclude thtat a consequences of DNA repair in CHO cells is modification rather that reemoval of cylobutane dimers.",

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AU - Thompson, Larry H.

AU - Clarkson, Judith M.

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10.1016/0167-8817(88)90007-7