Abstract
Introducing structural modifications into biomolecules represents a powerful approach to dissect their functions and roles in biological processes. Bacterial polysaccharides, despite their rich structural information and essential roles in bacterium-host interactions and bacterial virulence, have largely been unexplored for in vivo structural modifications. In this study, we demonstrate the incorporation of a panel of monosaccharide analogs into bacterial polysaccharides in a highly homogenous manner via metabolic engineering of a promiscuous sugar nucleotide biosynthetic pathway. In addition, the bioorthorgonal functional groups metabolically incorporated were exploited for cell surface labeling using in vitro selective chemical ligation reactions. In summary, our study presents a general, facile and effective approach for in vivo generation of novel tailor-made bacterial polysaccharides.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4207-4212 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 106 |
| Issue number | 11 |
| DOIs | |
| State | Published - Mar 17 2009 |
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Keywords
- Chemical remodeling
- Fucose
- Metabolic engineering
- Sugar nucleotide biosynthesis
ASJC Scopus subject areas
- General
Cite this
Remodeling bacterial polysaccharides by metabolic pathway engineering. / Yi, Wen; Liu, Xianwei; Li, Yanhong; Li, Jianjun; Xia, Chengfeng; Zhou, Guangyan; Zhang, Wenpeng; Zhao, Wei; Chen, Xi; Wang, Peng George.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 11, 17.03.2009, p. 4207-4212.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Remodeling bacterial polysaccharides by metabolic pathway engineering
AU - Yi, Wen
AU - Liu, Xianwei
AU - Li, Yanhong
AU - Li, Jianjun
AU - Xia, Chengfeng
AU - Zhou, Guangyan
AU - Zhang, Wenpeng
AU - Zhao, Wei
AU - Chen, Xi
AU - Wang, Peng George
PY - 2009/3/17
Y1 - 2009/3/17
N2 - Introducing structural modifications into biomolecules represents a powerful approach to dissect their functions and roles in biological processes. Bacterial polysaccharides, despite their rich structural information and essential roles in bacterium-host interactions and bacterial virulence, have largely been unexplored for in vivo structural modifications. In this study, we demonstrate the incorporation of a panel of monosaccharide analogs into bacterial polysaccharides in a highly homogenous manner via metabolic engineering of a promiscuous sugar nucleotide biosynthetic pathway. In addition, the bioorthorgonal functional groups metabolically incorporated were exploited for cell surface labeling using in vitro selective chemical ligation reactions. In summary, our study presents a general, facile and effective approach for in vivo generation of novel tailor-made bacterial polysaccharides.
AB - Introducing structural modifications into biomolecules represents a powerful approach to dissect their functions and roles in biological processes. Bacterial polysaccharides, despite their rich structural information and essential roles in bacterium-host interactions and bacterial virulence, have largely been unexplored for in vivo structural modifications. In this study, we demonstrate the incorporation of a panel of monosaccharide analogs into bacterial polysaccharides in a highly homogenous manner via metabolic engineering of a promiscuous sugar nucleotide biosynthetic pathway. In addition, the bioorthorgonal functional groups metabolically incorporated were exploited for cell surface labeling using in vitro selective chemical ligation reactions. In summary, our study presents a general, facile and effective approach for in vivo generation of novel tailor-made bacterial polysaccharides.
KW - Chemical remodeling
KW - Fucose
KW - Metabolic engineering
KW - Sugar nucleotide biosynthesis
UR - http://www.scopus.com/inward/record.url?scp=63149099211&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63149099211&partnerID=8YFLogxK
U2 - 10.1073/pnas.0812432106
DO - 10.1073/pnas.0812432106
M3 - Article
C2 - 19251666
AN - SCOPUS:63149099211
VL - 106
SP - 4207
EP - 4212
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 11
ER -