TY - JOUR
T1 - Release of the ρ subunit from Escherichia coli RNA polymerase transcription complexes is dependent on the promoter sequence
AU - Stackhouse, Thomas M.
AU - Telesnitsky, Alice P.
AU - Meares, Claude F.
PY - 1989
Y1 - 1989
N2 - The ρ subunit of bacterial RNA polymerase is required for the specific initiation of transcription at promoter sites. However, ρ is released from the transcription complex shortly after transcription is initiated, and elongation proceeds in the absence of ρ. In order to study the position of ρ release, we have developed a method to quantify the photoaffinity labeling produced by an aryl azide positioned at the leading (5′-) end of nascent RNA, as a function of the transcript length [Stackhouse, T. M., & Meares, C. F. (1988) Biochemistry 27, 3038-3045]. Here we compare photoaffinity labeling of transcription complexes containing three natural bacteriophage promoters (λ PR, λ PL, and T7 A1) and two recombinant constructs, A1/PR (T7 A1 promoter with the λ PR transcribed region) and PR/A1 (λ PR promoter with the T7 A1 transcribed region). Significant photoaffinity labeling of the ρ subunit was observed only on the templates containing the λ PR promoter region, regardless of the sequence of the transcribed region. These results indicate the molecular interactions responsible for the position of ρ release from the transcription complex mainly involve the nucleotide sequence of the promoter region - rather than the transcribed region - of the DNA template. Further studies on transcription complexes containing the A1/PR and the PR/A1 templates were performed, using polyclonal antibodies against the holoenzyme or against the ρ subunit. These experiments corroborate the promoter dependence of ρ release. They also show a correlation between the release of ρ and stable binding of the transcript by the transcription complex.
AB - The ρ subunit of bacterial RNA polymerase is required for the specific initiation of transcription at promoter sites. However, ρ is released from the transcription complex shortly after transcription is initiated, and elongation proceeds in the absence of ρ. In order to study the position of ρ release, we have developed a method to quantify the photoaffinity labeling produced by an aryl azide positioned at the leading (5′-) end of nascent RNA, as a function of the transcript length [Stackhouse, T. M., & Meares, C. F. (1988) Biochemistry 27, 3038-3045]. Here we compare photoaffinity labeling of transcription complexes containing three natural bacteriophage promoters (λ PR, λ PL, and T7 A1) and two recombinant constructs, A1/PR (T7 A1 promoter with the λ PR transcribed region) and PR/A1 (λ PR promoter with the T7 A1 transcribed region). Significant photoaffinity labeling of the ρ subunit was observed only on the templates containing the λ PR promoter region, regardless of the sequence of the transcribed region. These results indicate the molecular interactions responsible for the position of ρ release from the transcription complex mainly involve the nucleotide sequence of the promoter region - rather than the transcribed region - of the DNA template. Further studies on transcription complexes containing the A1/PR and the PR/A1 templates were performed, using polyclonal antibodies against the holoenzyme or against the ρ subunit. These experiments corroborate the promoter dependence of ρ release. They also show a correlation between the release of ρ and stable binding of the transcript by the transcription complex.
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M3 - Article
C2 - 2482069
AN - SCOPUS:0024974087
VL - 28
SP - 7781
EP - 7788
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 19
ER -