Release of cyclooxygenase products from primary cultures of tracheal epithelia of dog and human

Jonathan Widdicombe; I. F. Ueki; D. Emery; D. Margolskee; J. Yergey; J. A. Nadel

Release of cyclooxygenase products from primary cultures of tracheal epithelia of dog and human

Jonathan Widdicombe, I. F. Ueki, D. Emery, D. Margolskee, J. Yergey, J. A. Nadel

Research output: Contribution to journal › Article › peer-review

Abstract

Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E₂ and smaller increases in PGF(2α), 6-keto-PGF(1α), and thromboxane B₂ output. Isoproterenol, vasoactive intestinal peptide (VIP), metacholine, and leukotrienes C₄ and D₄ had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE₂ to PGD₂ released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.

Original language	English (US)
Journal	American Journal of Physiology - Lung Cellular and Molecular Physiology
Volume	257
Issue number	6
State	Published - 1989
Externally published	Yes

Keywords

bradykinin
calcium ionophore
chloride secretion
platelet-activating factor
prostaglandins

ASJC Scopus subject areas

Cell Biology
Physiology
Pulmonary and Respiratory Medicine

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title = "Release of cyclooxygenase products from primary cultures of tracheal epithelia of dog and human",

abstract = "Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF(2α), 6-keto-PGF(1α), and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), metacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.",

keywords = "bradykinin, calcium ionophore, chloride secretion, platelet-activating factor, prostaglandins",

author = "Jonathan Widdicombe and Ueki, {I. F.} and D. Emery and D. Margolskee and J. Yergey and Nadel, {J. A.}",

year = "1989",

language = "English (US)",

volume = "257",

journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",

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publisher = "American Physiological Society",

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T1 - Release of cyclooxygenase products from primary cultures of tracheal epithelia of dog and human

AU - Widdicombe, Jonathan

AU - Ueki, I. F.

AU - Emery, D.

AU - Margolskee, D.

AU - Yergey, J.

AU - Nadel, J. A.

PY - 1989

Y1 - 1989

N2 - Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF(2α), 6-keto-PGF(1α), and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), metacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.

AB - Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF(2α), 6-keto-PGF(1α), and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), metacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.

KW - bradykinin

KW - calcium ionophore

KW - chloride secretion

KW - platelet-activating factor

KW - prostaglandins

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VL - 257

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

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