Abstract
Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF(2α), 6-keto-PGF(1α), and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), metacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Lung Cellular and Molecular Physiology |
| Volume | 257 |
| Issue number | 6 |
| State | Published - 1989 |
| Externally published | Yes |
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Keywords
- bradykinin
- calcium ionophore
- chloride secretion
- platelet-activating factor
- prostaglandins
ASJC Scopus subject areas
- Cell Biology
- Physiology
- Pulmonary and Respiratory Medicine
Cite this
Release of cyclooxygenase products from primary cultures of tracheal epithelia of dog and human. / Widdicombe, Jonathan; Ueki, I. F.; Emery, D.; Margolskee, D.; Yergey, J.; Nadel, J. A.
In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 257, No. 6, 1989.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Release of cyclooxygenase products from primary cultures of tracheal epithelia of dog and human
AU - Widdicombe, Jonathan
AU - Ueki, I. F.
AU - Emery, D.
AU - Margolskee, D.
AU - Yergey, J.
AU - Nadel, J. A.
PY - 1989
Y1 - 1989
N2 - Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF(2α), 6-keto-PGF(1α), and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), metacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.
AB - Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF(2α), 6-keto-PGF(1α), and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), metacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.
KW - bradykinin
KW - calcium ionophore
KW - chloride secretion
KW - platelet-activating factor
KW - prostaglandins
UR - http://www.scopus.com/inward/record.url?scp=0024819892&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024819892&partnerID=8YFLogxK
M3 - Article
C2 - 2514604
AN - SCOPUS:0024819892
VL - 257
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 6
ER -