Abstract
Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF(2α), 6-keto-PGF(1α), and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), metacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.
Original language | English (US) |
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Journal | American Journal of Physiology - Lung Cellular and Molecular Physiology |
Volume | 257 |
Issue number | 6 |
State | Published - 1989 |
Externally published | Yes |
Keywords
- bradykinin
- calcium ionophore
- chloride secretion
- platelet-activating factor
- prostaglandins
ASJC Scopus subject areas
- Cell Biology
- Physiology
- Pulmonary and Respiratory Medicine