Release of cyclooxygenase products from primary cultures of tracheal epithelia of dog and human

Jonathan Widdicombe, I. F. Ueki, D. Emery, D. Margolskee, J. Yergey, J. A. Nadel

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF(2α), 6-keto-PGF(1α), and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), metacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume257
Issue number6
StatePublished - 1989
Externally publishedYes

Fingerprint

Calcimycin
Prostaglandin-Endoperoxide Synthases
Bradykinin
Epithelium
Dogs
Platelet Activating Factor
Vasoactive Intestinal Peptide
Prostaglandins F
Isoproterenol
Dinoprostone
Leukotriene D4
Prostaglandin D2
Leukotriene C4
Thromboxane B2
Methacholine Chloride
Calcium Ionophores
Indomethacin
Gas Chromatography-Mass Spectrometry
Radioimmunoassay

Keywords

  • bradykinin
  • calcium ionophore
  • chloride secretion
  • platelet-activating factor
  • prostaglandins

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Release of cyclooxygenase products from primary cultures of tracheal epithelia of dog and human. / Widdicombe, Jonathan; Ueki, I. F.; Emery, D.; Margolskee, D.; Yergey, J.; Nadel, J. A.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 257, No. 6, 1989.

Research output: Contribution to journalArticle

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AU - Ueki, I. F.

AU - Emery, D.

AU - Margolskee, D.

AU - Yergey, J.

AU - Nadel, J. A.

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N2 - Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF(2α), 6-keto-PGF(1α), and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), metacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.

AB - Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF(2α), 6-keto-PGF(1α), and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), metacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.

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