Relaxin secretion by human granulosa cell culture is predictive of in-vitro fertilization-embryo transfer success

Dennis R. Stewart, Catherine A. VandeVoort

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

We have developed a cell culture system for human luteinizing granulosa cells which supports the timely and dynamic secretion of oestrogen, progesterone and relaxin in patterns that mimic serum concentrations of these hormones during the luteal phase of the menstrual cycle. There was a wide variation in the amount of relaxin secreted by the cultured cells for the 69 patients studied. As relaxin production was generally maximal by day 10 of culture, comparisons were made at this time point. It was observed that most of the conceptions occurred in patients with higher relaxin secretion in vitro. All cycles with relaxin > 800 pg/ml on day 10 had a term pregnancy while only 13% of cycles with relaxin < 200 pg/ml had term pregnancies. A limited number of cycles from donor/recipient cycles did not show similar results. Steroid concentrations were not predictive of conception. These results demonstrated that in-vitro production of relaxin is predictive of implantation success in in-vitro fertilization (IVF)-embryo transfer cycles. This supports the hypothesis that relaxin may be involved in implantation and that lowered relaxin concentrations may be a partial cause of poor pregnancy rates after IVF.

Original languageEnglish (US)
Pages (from-to)338-344
Number of pages7
JournalHuman Reproduction
Volume14
Issue number2
StatePublished - 1999

Keywords

  • Granulosa cell culture
  • In-vitro fertilization
  • Infertility
  • Pregnancy
  • Relaxin

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynecology
  • Reproductive Medicine

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