Relaxation of arterial smooth muscle by calcium sparks

M. T. Nelson, H. Cheng, M. Rubart, Luis Fernando Santana, A. D. Bonev, H. J. Knot, W. J. Lederer

Research output: Contribution to journalArticle

1089 Citations (Scopus)

Abstract

Local increases in intracellular calcium ion concentration ([Ca2+](i)) resulting from activation of the ryanodine-sensitive calcium-release channel in the sarcoplasmic reticulum (SR) of smooth muscle cause arterial dilation. Ryanodine-sensitive, spontaneous local increases in [Ca2+](i) (Ca2+ sparks) from the SR were observed just under the surface membrane of single smooth muscle cells from myogenic cerebral arteries. Ryanodine and thapsigargin inhibited Ca2+ sparks and Ca2+-dependent potassium (K(Ca)) currents, suggesting that Ca2+ sparks activate K(Ca) channels. Furthermore, K(Ca) channels activated by Ca2+ sparks appeared to hyperpolarize and dilate pressurized myogenic arteries because ryanodine and thapsigargin depolarized and constricted these arteries to an extent similar to that produced by blockers of K(Ca) channels. Ca2+ sparks indirectly cause vasodilation through activation of K(Ca) channels, but have little direct effect on spatially averaged [Ca2+](i), which regulates contraction.

Original languageEnglish (US)
Pages (from-to)633-637
Number of pages5
JournalScience
Volume270
Issue number5236
StatePublished - Oct 27 1995
Externally publishedYes

Fingerprint

Ryanodine
Calcium Signaling
Smooth Muscle
Thapsigargin
Sarcoplasmic Reticulum
Arteries
Calcium-Activated Potassium Channels
Cerebral Arteries
Calcium Channels
Vasodilation
Smooth Muscle Myocytes
Dilatation
Potassium
Ions
Calcium
Membranes

ASJC Scopus subject areas

  • General

Cite this

Nelson, M. T., Cheng, H., Rubart, M., Santana, L. F., Bonev, A. D., Knot, H. J., & Lederer, W. J. (1995). Relaxation of arterial smooth muscle by calcium sparks. Science, 270(5236), 633-637.

Relaxation of arterial smooth muscle by calcium sparks. / Nelson, M. T.; Cheng, H.; Rubart, M.; Santana, Luis Fernando; Bonev, A. D.; Knot, H. J.; Lederer, W. J.

In: Science, Vol. 270, No. 5236, 27.10.1995, p. 633-637.

Research output: Contribution to journalArticle

Nelson, MT, Cheng, H, Rubart, M, Santana, LF, Bonev, AD, Knot, HJ & Lederer, WJ 1995, 'Relaxation of arterial smooth muscle by calcium sparks', Science, vol. 270, no. 5236, pp. 633-637.
Nelson MT, Cheng H, Rubart M, Santana LF, Bonev AD, Knot HJ et al. Relaxation of arterial smooth muscle by calcium sparks. Science. 1995 Oct 27;270(5236):633-637.
Nelson, M. T. ; Cheng, H. ; Rubart, M. ; Santana, Luis Fernando ; Bonev, A. D. ; Knot, H. J. ; Lederer, W. J. / Relaxation of arterial smooth muscle by calcium sparks. In: Science. 1995 ; Vol. 270, No. 5236. pp. 633-637.
@article{abca419b471b461e99221a65029e254f,
title = "Relaxation of arterial smooth muscle by calcium sparks",
abstract = "Local increases in intracellular calcium ion concentration ([Ca2+](i)) resulting from activation of the ryanodine-sensitive calcium-release channel in the sarcoplasmic reticulum (SR) of smooth muscle cause arterial dilation. Ryanodine-sensitive, spontaneous local increases in [Ca2+](i) (Ca2+ sparks) from the SR were observed just under the surface membrane of single smooth muscle cells from myogenic cerebral arteries. Ryanodine and thapsigargin inhibited Ca2+ sparks and Ca2+-dependent potassium (K(Ca)) currents, suggesting that Ca2+ sparks activate K(Ca) channels. Furthermore, K(Ca) channels activated by Ca2+ sparks appeared to hyperpolarize and dilate pressurized myogenic arteries because ryanodine and thapsigargin depolarized and constricted these arteries to an extent similar to that produced by blockers of K(Ca) channels. Ca2+ sparks indirectly cause vasodilation through activation of K(Ca) channels, but have little direct effect on spatially averaged [Ca2+](i), which regulates contraction.",
author = "Nelson, {M. T.} and H. Cheng and M. Rubart and Santana, {Luis Fernando} and Bonev, {A. D.} and Knot, {H. J.} and Lederer, {W. J.}",
year = "1995",
month = "10",
day = "27",
language = "English (US)",
volume = "270",
pages = "633--637",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5236",

}

TY - JOUR

T1 - Relaxation of arterial smooth muscle by calcium sparks

AU - Nelson, M. T.

AU - Cheng, H.

AU - Rubart, M.

AU - Santana, Luis Fernando

AU - Bonev, A. D.

AU - Knot, H. J.

AU - Lederer, W. J.

PY - 1995/10/27

Y1 - 1995/10/27

N2 - Local increases in intracellular calcium ion concentration ([Ca2+](i)) resulting from activation of the ryanodine-sensitive calcium-release channel in the sarcoplasmic reticulum (SR) of smooth muscle cause arterial dilation. Ryanodine-sensitive, spontaneous local increases in [Ca2+](i) (Ca2+ sparks) from the SR were observed just under the surface membrane of single smooth muscle cells from myogenic cerebral arteries. Ryanodine and thapsigargin inhibited Ca2+ sparks and Ca2+-dependent potassium (K(Ca)) currents, suggesting that Ca2+ sparks activate K(Ca) channels. Furthermore, K(Ca) channels activated by Ca2+ sparks appeared to hyperpolarize and dilate pressurized myogenic arteries because ryanodine and thapsigargin depolarized and constricted these arteries to an extent similar to that produced by blockers of K(Ca) channels. Ca2+ sparks indirectly cause vasodilation through activation of K(Ca) channels, but have little direct effect on spatially averaged [Ca2+](i), which regulates contraction.

AB - Local increases in intracellular calcium ion concentration ([Ca2+](i)) resulting from activation of the ryanodine-sensitive calcium-release channel in the sarcoplasmic reticulum (SR) of smooth muscle cause arterial dilation. Ryanodine-sensitive, spontaneous local increases in [Ca2+](i) (Ca2+ sparks) from the SR were observed just under the surface membrane of single smooth muscle cells from myogenic cerebral arteries. Ryanodine and thapsigargin inhibited Ca2+ sparks and Ca2+-dependent potassium (K(Ca)) currents, suggesting that Ca2+ sparks activate K(Ca) channels. Furthermore, K(Ca) channels activated by Ca2+ sparks appeared to hyperpolarize and dilate pressurized myogenic arteries because ryanodine and thapsigargin depolarized and constricted these arteries to an extent similar to that produced by blockers of K(Ca) channels. Ca2+ sparks indirectly cause vasodilation through activation of K(Ca) channels, but have little direct effect on spatially averaged [Ca2+](i), which regulates contraction.

UR - http://www.scopus.com/inward/record.url?scp=0028807878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028807878&partnerID=8YFLogxK

M3 - Article

C2 - 7570021

AN - SCOPUS:0028807878

VL - 270

SP - 633

EP - 637

JO - Science

JF - Science

SN - 0036-8075

IS - 5236

ER -