Relaxation in ferret ventricular myocytes: role of the sarcolemmal Ca ATPase

Rosana A. Bassani, José W M Bassani, Donald M Bers

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

In ferret ventricular myocytes the rate of intracellular Ca concentration [Ca]i decline and relaxation is remarkably fast (compared with rabbit and rat) under conditions where both the sarcoplasmic reticulum Ca uptake and Na/Ca exchange are inhibited. Here we explore the possibility that this rapid [Ca]i decline in ferret cells is attributable to the sarcolemmal Ca ATPase by using carboxyeosin (a potent inhibitor of the sarcolemmal Ca-ATPase). We compare the effects of carboxyeosin with those of elevated extracellular [Ca] ([Ca]o) (a thermodynamic approach to limit Ca transport by the sarcolemmal Ca ATPase). In rabbit cells, carboxyeosin and high [Ca]o slowed [Ca]i decline similarly and both virtually abolished [Ca]i decline when mitochondrial Ca uptake was also inhibited. In ferret cells, carboxyeosin treatment produced these same effects on [Ca]i decline, but high [Ca]o did not mimic them. Moreover, only in carboxyeosintreated ferret cells did additional inhibition of mitochondrial Ca uptake nearly abolish [Ca]i decline. We conclude that, carboxyeosin loading can inhibit the sarcolemmal Ca-ATPase in intact myocytes; that this pump seems likely to be responsible for the much faster relaxation observed in ferret cells after block of SR Ca accumulation and Na/Ca exchange transport and that the sarcolemmal Ca pump apparently has different characteristics in rabbit and ferret ventricular myocytes.

Original languageEnglish (US)
Pages (from-to)573-578
Number of pages6
JournalPflügers Archiv European Journal of Physiology
Volume430
Issue number4
DOIs
StatePublished - Aug 1995
Externally publishedYes

Keywords

  • [Ca] decline
  • Ca transport
  • Carboxyeosin
  • Species differences

ASJC Scopus subject areas

  • Physiology

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