Abstract
The relative efficacy of 10 flavonoid compounds and some common antioxidants in inhibiting 12-lipoxygenase of fish gill was determined. Lipoxygenase activity was measured by oxygen consumption with polarography and formation of hydroxy fatty acid using thin layer chromatography with autoradiography. Generally, the inhibition of 12-lipoxygenase by flavonoids was noncompetitive. The lipoxygenase product pattern did not change in the presence of flavonoids. Fisetin (3,3′,4′,7-tetrahydroxyflavone) and quercetin (3,3′,4′,5,7-pentahydroxyflavone) were the most potent inhibitors of 12-lipoxygenase, with IC50's of 0.25 and 0.4 μM respectively. These were compared with IC50's values of 5, 1000 μM for BHA (t-butylhydroxyanisole) and BHT (t-butylhydroxytoluene) respectively. Possible inhibitory mechanisms and relationships between flavonoid structure and inhibitory potencies are discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 322-326 |
| Number of pages | 5 |
| Journal | Lipids |
| Volume | 23 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Food Science
- Organic Chemistry
- Cell Biology
Cite this
Relative inhibitory potencies of flavonoids on 12-lipoxygenase of fish gill. / Hsieh, R. J.; German, J. B.; Kinsella, J. E.
In: Lipids, Vol. 23, No. 4, 04.1988, p. 322-326.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Relative inhibitory potencies of flavonoids on 12-lipoxygenase of fish gill
AU - Hsieh, R. J.
AU - German, J. B.
AU - Kinsella, J. E.
PY - 1988/4
Y1 - 1988/4
N2 - The relative efficacy of 10 flavonoid compounds and some common antioxidants in inhibiting 12-lipoxygenase of fish gill was determined. Lipoxygenase activity was measured by oxygen consumption with polarography and formation of hydroxy fatty acid using thin layer chromatography with autoradiography. Generally, the inhibition of 12-lipoxygenase by flavonoids was noncompetitive. The lipoxygenase product pattern did not change in the presence of flavonoids. Fisetin (3,3′,4′,7-tetrahydroxyflavone) and quercetin (3,3′,4′,5,7-pentahydroxyflavone) were the most potent inhibitors of 12-lipoxygenase, with IC50's of 0.25 and 0.4 μM respectively. These were compared with IC50's values of 5, 1000 μM for BHA (t-butylhydroxyanisole) and BHT (t-butylhydroxytoluene) respectively. Possible inhibitory mechanisms and relationships between flavonoid structure and inhibitory potencies are discussed.
AB - The relative efficacy of 10 flavonoid compounds and some common antioxidants in inhibiting 12-lipoxygenase of fish gill was determined. Lipoxygenase activity was measured by oxygen consumption with polarography and formation of hydroxy fatty acid using thin layer chromatography with autoradiography. Generally, the inhibition of 12-lipoxygenase by flavonoids was noncompetitive. The lipoxygenase product pattern did not change in the presence of flavonoids. Fisetin (3,3′,4′,7-tetrahydroxyflavone) and quercetin (3,3′,4′,5,7-pentahydroxyflavone) were the most potent inhibitors of 12-lipoxygenase, with IC50's of 0.25 and 0.4 μM respectively. These were compared with IC50's values of 5, 1000 μM for BHA (t-butylhydroxyanisole) and BHT (t-butylhydroxytoluene) respectively. Possible inhibitory mechanisms and relationships between flavonoid structure and inhibitory potencies are discussed.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023895319&partnerID=8YFLogxK
U2 - 10.1007/BF02537342
DO - 10.1007/BF02537342
M3 - Article
C2 - 3398718
AN - SCOPUS:0023895319
VL - 23
SP - 322
EP - 326
JO - Lipids
JF - Lipids
SN - 0024-4201
IS - 4
ER -