Abstract
The relative efficacy of 10 flavonoid compounds and some common antioxidants in inhibiting 12-lipoxygenase of fish gill was determined. Lipoxygenase activity was measured by oxygen consumption with polarography and formation of hydroxy fatty acid using thin layer chromatography with autoradiography. Generally, the inhibition of 12-lipoxygenase by flavonoids was noncompetitive. The lipoxygenase product pattern did not change in the presence of flavonoids. Fisetin (3,3′,4′,7-tetrahydroxyflavone) and quercetin (3,3′,4′,5,7-pentahydroxyflavone) were the most potent inhibitors of 12-lipoxygenase, with IC50's of 0.25 and 0.4 μM respectively. These were compared with IC50's values of 5, 1000 μM for BHA (t-butylhydroxyanisole) and BHT (t-butylhydroxytoluene) respectively. Possible inhibitory mechanisms and relationships between flavonoid structure and inhibitory potencies are discussed.
Original language | English (US) |
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Pages (from-to) | 322-326 |
Number of pages | 5 |
Journal | Lipids |
Volume | 23 |
Issue number | 4 |
DOIs | |
State | Published - Apr 1988 |
Externally published | Yes |
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Food Science
- Organic Chemistry
- Cell Biology