Relative inhibitory potencies of flavonoids on 12-lipoxygenase of fish gill

R. J. Hsieh, J. B. German, J. E. Kinsella

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The relative efficacy of 10 flavonoid compounds and some common antioxidants in inhibiting 12-lipoxygenase of fish gill was determined. Lipoxygenase activity was measured by oxygen consumption with polarography and formation of hydroxy fatty acid using thin layer chromatography with autoradiography. Generally, the inhibition of 12-lipoxygenase by flavonoids was noncompetitive. The lipoxygenase product pattern did not change in the presence of flavonoids. Fisetin (3,3′,4′,7-tetrahydroxyflavone) and quercetin (3,3′,4′,5,7-pentahydroxyflavone) were the most potent inhibitors of 12-lipoxygenase, with IC50's of 0.25 and 0.4 μM respectively. These were compared with IC50's values of 5, 1000 μM for BHA (t-butylhydroxyanisole) and BHT (t-butylhydroxytoluene) respectively. Possible inhibitory mechanisms and relationships between flavonoid structure and inhibitory potencies are discussed.

Original languageEnglish (US)
Pages (from-to)322-326
Number of pages5
JournalLipids
Volume23
Issue number4
DOIs
StatePublished - Apr 1988
Externally publishedYes

Fingerprint

Arachidonate 12-Lipoxygenase
lipoxygenase
Flavonoids
Fish
gills
Fishes
flavonoids
Lipoxygenase
Quercetin
fish
Inhibitory Concentration 50
inhibitory concentration 50
Polarography
Butylated Hydroxyanisole
Polarographic analysis
Lipoxygenase Inhibitors
Thin layer chromatography
Butylated Hydroxytoluene
Hydroxy Acids
hydroxy fatty acids

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science
  • Organic Chemistry
  • Cell Biology

Cite this

Relative inhibitory potencies of flavonoids on 12-lipoxygenase of fish gill. / Hsieh, R. J.; German, J. B.; Kinsella, J. E.

In: Lipids, Vol. 23, No. 4, 04.1988, p. 322-326.

Research output: Contribution to journalArticle

Hsieh, RJ, German, JB & Kinsella, JE 1988, 'Relative inhibitory potencies of flavonoids on 12-lipoxygenase of fish gill', Lipids, vol. 23, no. 4, pp. 322-326. https://doi.org/10.1007/BF02537342
Hsieh, R. J. ; German, J. B. ; Kinsella, J. E. / Relative inhibitory potencies of flavonoids on 12-lipoxygenase of fish gill. In: Lipids. 1988 ; Vol. 23, No. 4. pp. 322-326.
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