Relative inhibitory potencies of flavonoids on 12-lipoxygenase of fish gill

R. J. Hsieh; J. B. German; J. E. Kinsella

doi:10.1007/BF02537342

Relative inhibitory potencies of flavonoids on 12-lipoxygenase of fish gill

R. J. Hsieh, J. B. German, J. E. Kinsella

Research output: Contribution to journal › Article

28 Scopus citations

Abstract

The relative efficacy of 10 flavonoid compounds and some common antioxidants in inhibiting 12-lipoxygenase of fish gill was determined. Lipoxygenase activity was measured by oxygen consumption with polarography and formation of hydroxy fatty acid using thin layer chromatography with autoradiography. Generally, the inhibition of 12-lipoxygenase by flavonoids was noncompetitive. The lipoxygenase product pattern did not change in the presence of flavonoids. Fisetin (3,3′,4′,7-tetrahydroxyflavone) and quercetin (3,3′,4′,5,7-pentahydroxyflavone) were the most potent inhibitors of 12-lipoxygenase, with IC₅₀'s of 0.25 and 0.4 μM respectively. These were compared with IC₅₀'s values of 5, 1000 μM for BHA (t-butylhydroxyanisole) and BHT (t-butylhydroxytoluene) respectively. Possible inhibitory mechanisms and relationships between flavonoid structure and inhibitory potencies are discussed.

Original language	English (US)
Pages (from-to)	322-326
Number of pages	5
Journal	Lipids
Volume	23
Issue number	4
DOIs	https://doi.org/10.1007/BF02537342
State	Published - Apr 1988
Externally published	Yes

ASJC Scopus subject areas

Medicine (miscellaneous)
Biochemistry
Biochemistry, Genetics and Molecular Biology(all)
Food Science
Organic Chemistry
Cell Biology

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Hsieh, R. J., German, J. B., & Kinsella, J. E. (1988). Relative inhibitory potencies of flavonoids on 12-lipoxygenase of fish gill. Lipids, 23(4), 322-326. https://doi.org/10.1007/BF02537342

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abstract = "The relative efficacy of 10 flavonoid compounds and some common antioxidants in inhibiting 12-lipoxygenase of fish gill was determined. Lipoxygenase activity was measured by oxygen consumption with polarography and formation of hydroxy fatty acid using thin layer chromatography with autoradiography. Generally, the inhibition of 12-lipoxygenase by flavonoids was noncompetitive. The lipoxygenase product pattern did not change in the presence of flavonoids. Fisetin (3,3′,4′,7-tetrahydroxyflavone) and quercetin (3,3′,4′,5,7-pentahydroxyflavone) were the most potent inhibitors of 12-lipoxygenase, with IC50's of 0.25 and 0.4 μM respectively. These were compared with IC50's values of 5, 1000 μM for BHA (t-butylhydroxyanisole) and BHT (t-butylhydroxytoluene) respectively. Possible inhibitory mechanisms and relationships between flavonoid structure and inhibitory potencies are discussed.",

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N2 - The relative efficacy of 10 flavonoid compounds and some common antioxidants in inhibiting 12-lipoxygenase of fish gill was determined. Lipoxygenase activity was measured by oxygen consumption with polarography and formation of hydroxy fatty acid using thin layer chromatography with autoradiography. Generally, the inhibition of 12-lipoxygenase by flavonoids was noncompetitive. The lipoxygenase product pattern did not change in the presence of flavonoids. Fisetin (3,3′,4′,7-tetrahydroxyflavone) and quercetin (3,3′,4′,5,7-pentahydroxyflavone) were the most potent inhibitors of 12-lipoxygenase, with IC50's of 0.25 and 0.4 μM respectively. These were compared with IC50's values of 5, 1000 μM for BHA (t-butylhydroxyanisole) and BHT (t-butylhydroxytoluene) respectively. Possible inhibitory mechanisms and relationships between flavonoid structure and inhibitory potencies are discussed.

AB - The relative efficacy of 10 flavonoid compounds and some common antioxidants in inhibiting 12-lipoxygenase of fish gill was determined. Lipoxygenase activity was measured by oxygen consumption with polarography and formation of hydroxy fatty acid using thin layer chromatography with autoradiography. Generally, the inhibition of 12-lipoxygenase by flavonoids was noncompetitive. The lipoxygenase product pattern did not change in the presence of flavonoids. Fisetin (3,3′,4′,7-tetrahydroxyflavone) and quercetin (3,3′,4′,5,7-pentahydroxyflavone) were the most potent inhibitors of 12-lipoxygenase, with IC50's of 0.25 and 0.4 μM respectively. These were compared with IC50's values of 5, 1000 μM for BHA (t-butylhydroxyanisole) and BHT (t-butylhydroxytoluene) respectively. Possible inhibitory mechanisms and relationships between flavonoid structure and inhibitory potencies are discussed.

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