TY - JOUR
T1 - Relationship of plasma leptin to plasma insulin and adiposity in normal weight and overweight women
T2 - Effects of dietary fat content and sustained weight loss
AU - Havel, Peter J
AU - Karakas, Siddika E
AU - Mueller, Wendy
AU - Johnson, Patricia R.
AU - Gingerich, Ronald L.
AU - Stern, Judith S.
PY - 1996
Y1 - 1996
N2 - Leptin, the product of the human homologue of the ob gene, which is defective in the obese (ob/ob) mouse, may be a humoral regulator of human adiposity. Plasma leptin concentrations were measured by RIA in 19 normal weight [body mass index (BMI) = 24.5 ± 0.6 kg/m2] and 19 overweight to obese (BMI = 34.7 ± 1.2 kg/m2) nondiabetic postmenopausal women on sequential controlled weight-maintaining diets containing 31%, 23%, and 14% of energy as fat, each for 4-6 weeks. Thereafter, the subjects ate a very low fat diet (<15%) ad libitum; plasma leptin and insulin concentrations, BMI, percent body fat (%BF), and resting energy expenditure were determined after 6 and 8 months. Absolute and adiposity-corrected plasma leptin levels were higher in overweight/obese women (37.7 ± 3.5 ng/mL; 1.01 ± 0.07 ng · mL- 1%BF-1) than in normal weight women (16.9 ± 2.2 ng/mL; 0.57 ± 0.06 ng · mL-1%BF-1, both P < 0.005 vs. obese), but were not different between the 31%, 23%, and 14% fat diets when body weight was stable. Plasma leptin was highly correlated with BMI (r = 0.81, P < 0.0001), %BF (r = 0.80, P < 0.0001), and fasting plasma insulin (r = 0.61, P < 0.0001). After 8 months on the ad libitum low fat diet, the women had lost an average of 6.9 ± 1.0% of body mass (-2.0 ± 0.3 kg/m2, P < 0.0001). In 15 subjects who lost more than 7% of body mass (-12.3 ± 1.0%), plasma leptin concentrations decreased (- 9.6 ± 1.9 ng/mL, P < 0.0005), and the decrease of plasma leptin per change of adiposity (Δleptin/Δ%BF) was greater in overweight/obese women (3.6 ± 0.5) than in normal weight women (0.9 ± 0.4, P < 0.01 vs. obese). In 18 other subjects who lost less than 7% of body mass (-2.7 ± 0.6%), plasma leptin was unchanged (+1.4 ± 1.4 ng/mL). Overall, the change of plasma leptin was significantly correlated with change of BMI (r = 0.43, P < 0.02), the change of %BF (r = 0.49, P < 0.005), the change of resting energy expenditure (r = 0.40, P < 0.02), and with the change of plasma insulin independently of changes of body adiposity (r = 0.45, P < 0.01). We conclude that plasma leptin concentrations are: 1) not affected by dietary fat content per se; 2) highly correlated with BMI, %BF, and plasma insulin in both overweight/obese and normal weight women; 3) decreased in parallel with plasma insulin after sustained weight loss; and 4) decreased more in overweight/obese than in normal weight women.
AB - Leptin, the product of the human homologue of the ob gene, which is defective in the obese (ob/ob) mouse, may be a humoral regulator of human adiposity. Plasma leptin concentrations were measured by RIA in 19 normal weight [body mass index (BMI) = 24.5 ± 0.6 kg/m2] and 19 overweight to obese (BMI = 34.7 ± 1.2 kg/m2) nondiabetic postmenopausal women on sequential controlled weight-maintaining diets containing 31%, 23%, and 14% of energy as fat, each for 4-6 weeks. Thereafter, the subjects ate a very low fat diet (<15%) ad libitum; plasma leptin and insulin concentrations, BMI, percent body fat (%BF), and resting energy expenditure were determined after 6 and 8 months. Absolute and adiposity-corrected plasma leptin levels were higher in overweight/obese women (37.7 ± 3.5 ng/mL; 1.01 ± 0.07 ng · mL- 1%BF-1) than in normal weight women (16.9 ± 2.2 ng/mL; 0.57 ± 0.06 ng · mL-1%BF-1, both P < 0.005 vs. obese), but were not different between the 31%, 23%, and 14% fat diets when body weight was stable. Plasma leptin was highly correlated with BMI (r = 0.81, P < 0.0001), %BF (r = 0.80, P < 0.0001), and fasting plasma insulin (r = 0.61, P < 0.0001). After 8 months on the ad libitum low fat diet, the women had lost an average of 6.9 ± 1.0% of body mass (-2.0 ± 0.3 kg/m2, P < 0.0001). In 15 subjects who lost more than 7% of body mass (-12.3 ± 1.0%), plasma leptin concentrations decreased (- 9.6 ± 1.9 ng/mL, P < 0.0005), and the decrease of plasma leptin per change of adiposity (Δleptin/Δ%BF) was greater in overweight/obese women (3.6 ± 0.5) than in normal weight women (0.9 ± 0.4, P < 0.01 vs. obese). In 18 other subjects who lost less than 7% of body mass (-2.7 ± 0.6%), plasma leptin was unchanged (+1.4 ± 1.4 ng/mL). Overall, the change of plasma leptin was significantly correlated with change of BMI (r = 0.43, P < 0.02), the change of %BF (r = 0.49, P < 0.005), the change of resting energy expenditure (r = 0.40, P < 0.02), and with the change of plasma insulin independently of changes of body adiposity (r = 0.45, P < 0.01). We conclude that plasma leptin concentrations are: 1) not affected by dietary fat content per se; 2) highly correlated with BMI, %BF, and plasma insulin in both overweight/obese and normal weight women; 3) decreased in parallel with plasma insulin after sustained weight loss; and 4) decreased more in overweight/obese than in normal weight women.
UR - http://www.scopus.com/inward/record.url?scp=0029819643&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029819643&partnerID=8YFLogxK
U2 - 10.1210/jc.81.12.4406
DO - 10.1210/jc.81.12.4406
M3 - Article
C2 - 8954050
AN - SCOPUS:0029819643
VL - 81
SP - 4406
EP - 4413
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 12
ER -