Relationship of p53 and bcl-2 to prognosis in muscle-invasive transitional cell carcinoma of the bladder

Research output: Contribution to journalArticle

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Abstract

Purpose: We examined the presence of the p53 and Bcl-2 oncoproteins, as detected by immunohistochemistry, in muscle-invasive bladder cancer and correlated this with survival. Materials and Methods: Formalin-fixed cystectomy specimens from 41 consecutive patients with mean follow-up of 52 months were used. Five patients were either lost to follow-up or died of other diseases and were not included in the survival evaluation. Results: Eighteen of 36 patients died of metastatic transitional cell carcinoma. p53 immunostaining was found in 61% of patients. In 21 of 23 this staining was homogeneous, with more than 75% of cancer cells staining using a DO-1/DO-7 antibody cocktail. p53 staining was not correlated with stage (p >0.25) or grade (p <0.10) in these invasive cancer specimens. Contrary to recent studies p53 immunostaining was not correlated with disease-specific survival. Bcl-2 immunostaining was found in 28% of patients and was not correlated with grade (p >0.25) or disease-specific survival. No combination of p53 and Bcl- 2 staining gave added predictive information. Conclusions: Cytoplasmic Bcl-2 is found in a small percentage of these cancers and does not correlate with prognosis. Further, p53 molecular overexpression is detected in the majority of muscle-invasive bladder tumors as a field defect. However, in patients undergoing cystectomy, it does not correlate with prognosis.

Original languageEnglish (US)
Pages (from-to)1754-1757
Number of pages4
JournalJournal of Urology
Volume155
Issue number5
DOIs
StatePublished - May 1996

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Transitional Cell Carcinoma
Urinary Bladder
Muscles
Staining and Labeling
Cystectomy
Urinary Bladder Neoplasms
Survival
Oncogene Proteins
Lost to Follow-Up
Formaldehyde
Neoplasms
Immunohistochemistry
Antibodies

Keywords

  • bladder neoplasms
  • genes, p53
  • immunohistochemistry
  • oncogene proteins

ASJC Scopus subject areas

  • Urology

Cite this

Relationship of p53 and bcl-2 to prognosis in muscle-invasive transitional cell carcinoma of the bladder. / Glick, Seth H.; Howell, Lydia P; deVere White, Ralph W.

In: Journal of Urology, Vol. 155, No. 5, 05.1996, p. 1754-1757.

Research output: Contribution to journalArticle

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abstract = "Purpose: We examined the presence of the p53 and Bcl-2 oncoproteins, as detected by immunohistochemistry, in muscle-invasive bladder cancer and correlated this with survival. Materials and Methods: Formalin-fixed cystectomy specimens from 41 consecutive patients with mean follow-up of 52 months were used. Five patients were either lost to follow-up or died of other diseases and were not included in the survival evaluation. Results: Eighteen of 36 patients died of metastatic transitional cell carcinoma. p53 immunostaining was found in 61{\%} of patients. In 21 of 23 this staining was homogeneous, with more than 75{\%} of cancer cells staining using a DO-1/DO-7 antibody cocktail. p53 staining was not correlated with stage (p >0.25) or grade (p <0.10) in these invasive cancer specimens. Contrary to recent studies p53 immunostaining was not correlated with disease-specific survival. Bcl-2 immunostaining was found in 28{\%} of patients and was not correlated with grade (p >0.25) or disease-specific survival. No combination of p53 and Bcl- 2 staining gave added predictive information. Conclusions: Cytoplasmic Bcl-2 is found in a small percentage of these cancers and does not correlate with prognosis. Further, p53 molecular overexpression is detected in the majority of muscle-invasive bladder tumors as a field defect. However, in patients undergoing cystectomy, it does not correlate with prognosis.",
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AU - deVere White, Ralph W

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N2 - Purpose: We examined the presence of the p53 and Bcl-2 oncoproteins, as detected by immunohistochemistry, in muscle-invasive bladder cancer and correlated this with survival. Materials and Methods: Formalin-fixed cystectomy specimens from 41 consecutive patients with mean follow-up of 52 months were used. Five patients were either lost to follow-up or died of other diseases and were not included in the survival evaluation. Results: Eighteen of 36 patients died of metastatic transitional cell carcinoma. p53 immunostaining was found in 61% of patients. In 21 of 23 this staining was homogeneous, with more than 75% of cancer cells staining using a DO-1/DO-7 antibody cocktail. p53 staining was not correlated with stage (p >0.25) or grade (p <0.10) in these invasive cancer specimens. Contrary to recent studies p53 immunostaining was not correlated with disease-specific survival. Bcl-2 immunostaining was found in 28% of patients and was not correlated with grade (p >0.25) or disease-specific survival. No combination of p53 and Bcl- 2 staining gave added predictive information. Conclusions: Cytoplasmic Bcl-2 is found in a small percentage of these cancers and does not correlate with prognosis. Further, p53 molecular overexpression is detected in the majority of muscle-invasive bladder tumors as a field defect. However, in patients undergoing cystectomy, it does not correlate with prognosis.

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