Abstract
The arginine deiminase (ADI) pathway exists in many microorganisms and is one of the main characteristics that distinguish between Lactococcus lactis ssp. lactis and ssp. cremoris. The ADI pathway converts arginine to produce ammonia, carbon dioxide, and ATP. Induction of this pathway in relation to energy production is not well characterized. The presence of arginine changed the metabolism of strain ML3 in the media containing different levels of lactose. The interaction between lactose and arginine utilization changed the pH of the growth medium in a cyclic pattern. Transcriptional analysis showed that the first enzyme in the ADI pathway (arginine deiminase, arcA) was induced by pH rather than arginine addition. Analysis of the ratio arginine to lactose led to a statistical model that linked lactose, arginine, external pH, and the ADI pathway.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 253-258 |
| Number of pages | 6 |
| Journal | International Dairy Journal |
| Volume | 11 |
| Issue number | 4-7 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
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Keywords
- Arginine deiminase pathway (ADI)
- Arginine metabolism
- Lactococcus lactis ssp. lactis
- Lactose metabolism
ASJC Scopus subject areas
- Food Science
Cite this
Relationship of arginine and lactose utilization by Lactococcus lactis ssp. lactis ML3. / Chou, Lan szu; Weimer, Bart C; Cutler, Richard.
In: International Dairy Journal, Vol. 11, No. 4-7, 2001, p. 253-258.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Relationship of arginine and lactose utilization by Lactococcus lactis ssp. lactis ML3
AU - Chou, Lan szu
AU - Weimer, Bart C
AU - Cutler, Richard
PY - 2001
Y1 - 2001
N2 - The arginine deiminase (ADI) pathway exists in many microorganisms and is one of the main characteristics that distinguish between Lactococcus lactis ssp. lactis and ssp. cremoris. The ADI pathway converts arginine to produce ammonia, carbon dioxide, and ATP. Induction of this pathway in relation to energy production is not well characterized. The presence of arginine changed the metabolism of strain ML3 in the media containing different levels of lactose. The interaction between lactose and arginine utilization changed the pH of the growth medium in a cyclic pattern. Transcriptional analysis showed that the first enzyme in the ADI pathway (arginine deiminase, arcA) was induced by pH rather than arginine addition. Analysis of the ratio arginine to lactose led to a statistical model that linked lactose, arginine, external pH, and the ADI pathway.
AB - The arginine deiminase (ADI) pathway exists in many microorganisms and is one of the main characteristics that distinguish between Lactococcus lactis ssp. lactis and ssp. cremoris. The ADI pathway converts arginine to produce ammonia, carbon dioxide, and ATP. Induction of this pathway in relation to energy production is not well characterized. The presence of arginine changed the metabolism of strain ML3 in the media containing different levels of lactose. The interaction between lactose and arginine utilization changed the pH of the growth medium in a cyclic pattern. Transcriptional analysis showed that the first enzyme in the ADI pathway (arginine deiminase, arcA) was induced by pH rather than arginine addition. Analysis of the ratio arginine to lactose led to a statistical model that linked lactose, arginine, external pH, and the ADI pathway.
KW - Arginine deiminase pathway (ADI)
KW - Arginine metabolism
KW - Lactococcus lactis ssp. lactis
KW - Lactose metabolism
UR - http://www.scopus.com/inward/record.url?scp=0034872391&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034872391&partnerID=8YFLogxK
U2 - 10.1016/S0958-6946(01)00055-3
DO - 10.1016/S0958-6946(01)00055-3
M3 - Article
AN - SCOPUS:0034872391
VL - 11
SP - 253
EP - 258
JO - International Dairy Journal
JF - International Dairy Journal
SN - 0958-6946
IS - 4-7
ER -