Abstract
Macrophages (M∅) harvested from the peritoneal cavities of mice after thioglycolate stimulation could be infected with murine cytomegalovirus (MCMV), although the efficiency of infection was low. Sequential measurements of interferon (IFN) production by virus-infected M∅ were performed in an attempt to explain the characteristics of MCMV infection in the cell cultures. Infected M∅ produced moderate amounts of IFN, which was completely neutralized by anti-IFN-α/β serum. The IFN was detectable in cultures as early as 8 h after infection and was produced only by exposing M∅ to infectious virus. Production increased until 48 to 72 h and preceded virus production, which was initially detected 72 h after infection. Treatment of the M∅ cultures with anti-IFN-α/β resulted not only in a marked increase in virus production, as well as a shortening of the long eclipse period of MCMV infection, but also induced increases in the number of M∅ releasing MCMV (VR-M∅). Thus, the IFN produced in MCMV-infected M∅ (MCMV-M∅ IFN) appeared to suppress the production and spread of MCMV. The increase in the number of VR-M∅ observed was more resistant to anti-fIFN-α/β treatment than the production of infectious virus. Tha antiviral effect of MCMV-M∅ IFN on MCMV infection in mouse embryo fibroblasts was similar to that induced by IFN-α/β. Therefore, MCMV-M∅ IFN appeared to be more active in protecting against the spread of cell-free MCMV than of cell-associated virus. These differences in sensitivity to IFN action suggest that M∅ may have a role in the latency of MCMV and their production of IFN may facilitate the generation of latent infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2961-2971 |
| Number of pages | 11 |
| Journal | Journal of General Virology |
| Volume | 69 |
| Issue number | 12 |
| State | Published - 1988 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Virology
Cite this
Relationship between the production of murine cytomegalovirus and interferon in macrophages. / Yamaguchi, T.; Shinagawa, Y.; Pollard, Richard B.
In: Journal of General Virology, Vol. 69, No. 12, 1988, p. 2961-2971.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Relationship between the production of murine cytomegalovirus and interferon in macrophages
AU - Yamaguchi, T.
AU - Shinagawa, Y.
AU - Pollard, Richard B
PY - 1988
Y1 - 1988
N2 - Macrophages (M∅) harvested from the peritoneal cavities of mice after thioglycolate stimulation could be infected with murine cytomegalovirus (MCMV), although the efficiency of infection was low. Sequential measurements of interferon (IFN) production by virus-infected M∅ were performed in an attempt to explain the characteristics of MCMV infection in the cell cultures. Infected M∅ produced moderate amounts of IFN, which was completely neutralized by anti-IFN-α/β serum. The IFN was detectable in cultures as early as 8 h after infection and was produced only by exposing M∅ to infectious virus. Production increased until 48 to 72 h and preceded virus production, which was initially detected 72 h after infection. Treatment of the M∅ cultures with anti-IFN-α/β resulted not only in a marked increase in virus production, as well as a shortening of the long eclipse period of MCMV infection, but also induced increases in the number of M∅ releasing MCMV (VR-M∅). Thus, the IFN produced in MCMV-infected M∅ (MCMV-M∅ IFN) appeared to suppress the production and spread of MCMV. The increase in the number of VR-M∅ observed was more resistant to anti-fIFN-α/β treatment than the production of infectious virus. Tha antiviral effect of MCMV-M∅ IFN on MCMV infection in mouse embryo fibroblasts was similar to that induced by IFN-α/β. Therefore, MCMV-M∅ IFN appeared to be more active in protecting against the spread of cell-free MCMV than of cell-associated virus. These differences in sensitivity to IFN action suggest that M∅ may have a role in the latency of MCMV and their production of IFN may facilitate the generation of latent infection.
AB - Macrophages (M∅) harvested from the peritoneal cavities of mice after thioglycolate stimulation could be infected with murine cytomegalovirus (MCMV), although the efficiency of infection was low. Sequential measurements of interferon (IFN) production by virus-infected M∅ were performed in an attempt to explain the characteristics of MCMV infection in the cell cultures. Infected M∅ produced moderate amounts of IFN, which was completely neutralized by anti-IFN-α/β serum. The IFN was detectable in cultures as early as 8 h after infection and was produced only by exposing M∅ to infectious virus. Production increased until 48 to 72 h and preceded virus production, which was initially detected 72 h after infection. Treatment of the M∅ cultures with anti-IFN-α/β resulted not only in a marked increase in virus production, as well as a shortening of the long eclipse period of MCMV infection, but also induced increases in the number of M∅ releasing MCMV (VR-M∅). Thus, the IFN produced in MCMV-infected M∅ (MCMV-M∅ IFN) appeared to suppress the production and spread of MCMV. The increase in the number of VR-M∅ observed was more resistant to anti-fIFN-α/β treatment than the production of infectious virus. Tha antiviral effect of MCMV-M∅ IFN on MCMV infection in mouse embryo fibroblasts was similar to that induced by IFN-α/β. Therefore, MCMV-M∅ IFN appeared to be more active in protecting against the spread of cell-free MCMV than of cell-associated virus. These differences in sensitivity to IFN action suggest that M∅ may have a role in the latency of MCMV and their production of IFN may facilitate the generation of latent infection.
UR - http://www.scopus.com/inward/record.url?scp=0024241116&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024241116&partnerID=8YFLogxK
M3 - Article
C2 - 2462012
AN - SCOPUS:0024241116
VL - 69
SP - 2961
EP - 2971
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 12
ER -