Relationship between the production of murine cytomegalovirus and interferon in macrophages

T. Yamaguchi, Y. Shinagawa, Richard B Pollard

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Macrophages (M∅) harvested from the peritoneal cavities of mice after thioglycolate stimulation could be infected with murine cytomegalovirus (MCMV), although the efficiency of infection was low. Sequential measurements of interferon (IFN) production by virus-infected M∅ were performed in an attempt to explain the characteristics of MCMV infection in the cell cultures. Infected M∅ produced moderate amounts of IFN, which was completely neutralized by anti-IFN-α/β serum. The IFN was detectable in cultures as early as 8 h after infection and was produced only by exposing M∅ to infectious virus. Production increased until 48 to 72 h and preceded virus production, which was initially detected 72 h after infection. Treatment of the M∅ cultures with anti-IFN-α/β resulted not only in a marked increase in virus production, as well as a shortening of the long eclipse period of MCMV infection, but also induced increases in the number of M∅ releasing MCMV (VR-M∅). Thus, the IFN produced in MCMV-infected M∅ (MCMV-M∅ IFN) appeared to suppress the production and spread of MCMV. The increase in the number of VR-M∅ observed was more resistant to anti-fIFN-α/β treatment than the production of infectious virus. Tha antiviral effect of MCMV-M∅ IFN on MCMV infection in mouse embryo fibroblasts was similar to that induced by IFN-α/β. Therefore, MCMV-M∅ IFN appeared to be more active in protecting against the spread of cell-free MCMV than of cell-associated virus. These differences in sensitivity to IFN action suggest that M∅ may have a role in the latency of MCMV and their production of IFN may facilitate the generation of latent infection.

Original languageEnglish (US)
Pages (from-to)2961-2971
Number of pages11
JournalJournal of General Virology
Volume69
Issue number12
StatePublished - 1988
Externally publishedYes

Fingerprint

Muromegalovirus
Interferons
Macrophages
Viruses
Cytomegalovirus Infections
Infection
Thioglycolates
Satellite Viruses
Peritoneal Cavity
Antiviral Agents

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Relationship between the production of murine cytomegalovirus and interferon in macrophages. / Yamaguchi, T.; Shinagawa, Y.; Pollard, Richard B.

In: Journal of General Virology, Vol. 69, No. 12, 1988, p. 2961-2971.

Research output: Contribution to journalArticle

@article{5fd2fb3bb2974566955b7bed32ab025f,
title = "Relationship between the production of murine cytomegalovirus and interferon in macrophages",
abstract = "Macrophages (M∅) harvested from the peritoneal cavities of mice after thioglycolate stimulation could be infected with murine cytomegalovirus (MCMV), although the efficiency of infection was low. Sequential measurements of interferon (IFN) production by virus-infected M∅ were performed in an attempt to explain the characteristics of MCMV infection in the cell cultures. Infected M∅ produced moderate amounts of IFN, which was completely neutralized by anti-IFN-α/β serum. The IFN was detectable in cultures as early as 8 h after infection and was produced only by exposing M∅ to infectious virus. Production increased until 48 to 72 h and preceded virus production, which was initially detected 72 h after infection. Treatment of the M∅ cultures with anti-IFN-α/β resulted not only in a marked increase in virus production, as well as a shortening of the long eclipse period of MCMV infection, but also induced increases in the number of M∅ releasing MCMV (VR-M∅). Thus, the IFN produced in MCMV-infected M∅ (MCMV-M∅ IFN) appeared to suppress the production and spread of MCMV. The increase in the number of VR-M∅ observed was more resistant to anti-fIFN-α/β treatment than the production of infectious virus. Tha antiviral effect of MCMV-M∅ IFN on MCMV infection in mouse embryo fibroblasts was similar to that induced by IFN-α/β. Therefore, MCMV-M∅ IFN appeared to be more active in protecting against the spread of cell-free MCMV than of cell-associated virus. These differences in sensitivity to IFN action suggest that M∅ may have a role in the latency of MCMV and their production of IFN may facilitate the generation of latent infection.",
author = "T. Yamaguchi and Y. Shinagawa and Pollard, {Richard B}",
year = "1988",
language = "English (US)",
volume = "69",
pages = "2961--2971",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "12",

}

TY - JOUR

T1 - Relationship between the production of murine cytomegalovirus and interferon in macrophages

AU - Yamaguchi, T.

AU - Shinagawa, Y.

AU - Pollard, Richard B

PY - 1988

Y1 - 1988

N2 - Macrophages (M∅) harvested from the peritoneal cavities of mice after thioglycolate stimulation could be infected with murine cytomegalovirus (MCMV), although the efficiency of infection was low. Sequential measurements of interferon (IFN) production by virus-infected M∅ were performed in an attempt to explain the characteristics of MCMV infection in the cell cultures. Infected M∅ produced moderate amounts of IFN, which was completely neutralized by anti-IFN-α/β serum. The IFN was detectable in cultures as early as 8 h after infection and was produced only by exposing M∅ to infectious virus. Production increased until 48 to 72 h and preceded virus production, which was initially detected 72 h after infection. Treatment of the M∅ cultures with anti-IFN-α/β resulted not only in a marked increase in virus production, as well as a shortening of the long eclipse period of MCMV infection, but also induced increases in the number of M∅ releasing MCMV (VR-M∅). Thus, the IFN produced in MCMV-infected M∅ (MCMV-M∅ IFN) appeared to suppress the production and spread of MCMV. The increase in the number of VR-M∅ observed was more resistant to anti-fIFN-α/β treatment than the production of infectious virus. Tha antiviral effect of MCMV-M∅ IFN on MCMV infection in mouse embryo fibroblasts was similar to that induced by IFN-α/β. Therefore, MCMV-M∅ IFN appeared to be more active in protecting against the spread of cell-free MCMV than of cell-associated virus. These differences in sensitivity to IFN action suggest that M∅ may have a role in the latency of MCMV and their production of IFN may facilitate the generation of latent infection.

AB - Macrophages (M∅) harvested from the peritoneal cavities of mice after thioglycolate stimulation could be infected with murine cytomegalovirus (MCMV), although the efficiency of infection was low. Sequential measurements of interferon (IFN) production by virus-infected M∅ were performed in an attempt to explain the characteristics of MCMV infection in the cell cultures. Infected M∅ produced moderate amounts of IFN, which was completely neutralized by anti-IFN-α/β serum. The IFN was detectable in cultures as early as 8 h after infection and was produced only by exposing M∅ to infectious virus. Production increased until 48 to 72 h and preceded virus production, which was initially detected 72 h after infection. Treatment of the M∅ cultures with anti-IFN-α/β resulted not only in a marked increase in virus production, as well as a shortening of the long eclipse period of MCMV infection, but also induced increases in the number of M∅ releasing MCMV (VR-M∅). Thus, the IFN produced in MCMV-infected M∅ (MCMV-M∅ IFN) appeared to suppress the production and spread of MCMV. The increase in the number of VR-M∅ observed was more resistant to anti-fIFN-α/β treatment than the production of infectious virus. Tha antiviral effect of MCMV-M∅ IFN on MCMV infection in mouse embryo fibroblasts was similar to that induced by IFN-α/β. Therefore, MCMV-M∅ IFN appeared to be more active in protecting against the spread of cell-free MCMV than of cell-associated virus. These differences in sensitivity to IFN action suggest that M∅ may have a role in the latency of MCMV and their production of IFN may facilitate the generation of latent infection.

UR - http://www.scopus.com/inward/record.url?scp=0024241116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024241116&partnerID=8YFLogxK

M3 - Article

VL - 69

SP - 2961

EP - 2971

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 12

ER -