Abstract
Objective - To validate a recently developed commercially available leptin radioimmunoassay (RIA) for use with feline serum and evaluate the relationship between serum leptin concentrations and body fat mass in domestic cats. Animals - 19 sexually intact male specific-pathogen-free domestic cats that weighed 3.8 to 7.1 kg and were 1.1 to 3.5 years old. Procedure - Specificity for feline leptin was evaluated by use of gel filtration chromatography and reverse-phase high-performance liquid chromatography fractionation of serum. Body fat mass was determined by use of the deuterium oxide (D2O) dilution method. Serum water D2O enrichment was measured by use of gas-phase Fourier transform infrared spectroscopy. Results - Body fat mass and percentage body fat ranged from 0.3 to 2.3 kg and 7.5 to 34.9%, respectively. Serum leptin concentrations were lower in the unfed versus the fed state and ranged between 1.6 and 4.9 ng/ml human equivalent (HE); mean ± SD value was 2.9 ± 0.2 ng/ml HE. Leptin concentrations increased with increasing body fat mass and percentage of body fat. Conclusions-Leptin is in the serum of domestic cats in free (> 78%) and apparently bound forms. The relationship between body fat and serum leptin concentration was similar to that observed in humans and rodents and indicative of a lipostatic role for leptin in cats. Cats that have an overabundance of body fat appear to be less sensitive to the weight-normalizing action of leptin than cats of ideal body condition. (Am J Vet Res 2000;61:796-801) From the Department of Molecular Biosciences, School of Veterinary Medicine (Backus, Rogers) and the Department of Nutrition, College of Agricultural and Environmental Sciences (Havel), University of California, Davis, CA 95616; and Linco Research, St Louis, MO 63301 (Gingerich).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 796-801 |
| Number of pages | 6 |
| Journal | American Journal of Veterinary Research |
| Volume | 61 |
| Issue number | 7 |
| State | Published - Jul 2000 |
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ASJC Scopus subject areas
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Relationship between serum leptin immunoreactivity and body fat mass as estimated by use of a novel gas-phase Fourier transform infrared spectroscopy deuterium dilution method in cats. / Backus, Robert C.; Havel, Peter J; Gingerich, Ronald L.; Rogers, Quinton.
In: American Journal of Veterinary Research, Vol. 61, No. 7, 07.2000, p. 796-801.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Relationship between serum leptin immunoreactivity and body fat mass as estimated by use of a novel gas-phase Fourier transform infrared spectroscopy deuterium dilution method in cats
AU - Backus, Robert C.
AU - Havel, Peter J
AU - Gingerich, Ronald L.
AU - Rogers, Quinton
PY - 2000/7
Y1 - 2000/7
N2 - Objective - To validate a recently developed commercially available leptin radioimmunoassay (RIA) for use with feline serum and evaluate the relationship between serum leptin concentrations and body fat mass in domestic cats. Animals - 19 sexually intact male specific-pathogen-free domestic cats that weighed 3.8 to 7.1 kg and were 1.1 to 3.5 years old. Procedure - Specificity for feline leptin was evaluated by use of gel filtration chromatography and reverse-phase high-performance liquid chromatography fractionation of serum. Body fat mass was determined by use of the deuterium oxide (D2O) dilution method. Serum water D2O enrichment was measured by use of gas-phase Fourier transform infrared spectroscopy. Results - Body fat mass and percentage body fat ranged from 0.3 to 2.3 kg and 7.5 to 34.9%, respectively. Serum leptin concentrations were lower in the unfed versus the fed state and ranged between 1.6 and 4.9 ng/ml human equivalent (HE); mean ± SD value was 2.9 ± 0.2 ng/ml HE. Leptin concentrations increased with increasing body fat mass and percentage of body fat. Conclusions-Leptin is in the serum of domestic cats in free (> 78%) and apparently bound forms. The relationship between body fat and serum leptin concentration was similar to that observed in humans and rodents and indicative of a lipostatic role for leptin in cats. Cats that have an overabundance of body fat appear to be less sensitive to the weight-normalizing action of leptin than cats of ideal body condition. (Am J Vet Res 2000;61:796-801) From the Department of Molecular Biosciences, School of Veterinary Medicine (Backus, Rogers) and the Department of Nutrition, College of Agricultural and Environmental Sciences (Havel), University of California, Davis, CA 95616; and Linco Research, St Louis, MO 63301 (Gingerich).
AB - Objective - To validate a recently developed commercially available leptin radioimmunoassay (RIA) for use with feline serum and evaluate the relationship between serum leptin concentrations and body fat mass in domestic cats. Animals - 19 sexually intact male specific-pathogen-free domestic cats that weighed 3.8 to 7.1 kg and were 1.1 to 3.5 years old. Procedure - Specificity for feline leptin was evaluated by use of gel filtration chromatography and reverse-phase high-performance liquid chromatography fractionation of serum. Body fat mass was determined by use of the deuterium oxide (D2O) dilution method. Serum water D2O enrichment was measured by use of gas-phase Fourier transform infrared spectroscopy. Results - Body fat mass and percentage body fat ranged from 0.3 to 2.3 kg and 7.5 to 34.9%, respectively. Serum leptin concentrations were lower in the unfed versus the fed state and ranged between 1.6 and 4.9 ng/ml human equivalent (HE); mean ± SD value was 2.9 ± 0.2 ng/ml HE. Leptin concentrations increased with increasing body fat mass and percentage of body fat. Conclusions-Leptin is in the serum of domestic cats in free (> 78%) and apparently bound forms. The relationship between body fat and serum leptin concentration was similar to that observed in humans and rodents and indicative of a lipostatic role for leptin in cats. Cats that have an overabundance of body fat appear to be less sensitive to the weight-normalizing action of leptin than cats of ideal body condition. (Am J Vet Res 2000;61:796-801) From the Department of Molecular Biosciences, School of Veterinary Medicine (Backus, Rogers) and the Department of Nutrition, College of Agricultural and Environmental Sciences (Havel), University of California, Davis, CA 95616; and Linco Research, St Louis, MO 63301 (Gingerich).
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M3 - Article
C2 - 10895903
AN - SCOPUS:0034222954
VL - 61
SP - 796
EP - 801
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 7
ER -