Relationship between kdr mutation and resistance to pyrethroid and DDT insecticides in natural populations of Anopheles gambiae

Lisa Reimer, Etienne Fondjo, Salomon Patchoké, Brehima Diallo, Yoosook Lee, Arash Ng, Hamadou M. Ndjemai, Jean Atangana, Sekou F. Traore, Gregory C Lanzaro, Anthony J. Cornel

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The spread of insecticide resistance genes in Anopheles gambiae Giles sensu stricto threatens to compromise vector-based malaria control programs. Two mutations at the same locus in the voltage-gated sodium channel gene are known to confer knockdown resistance (kdr) to pyrethroids and DDT. Kdr-e involves a leucine-serine substitution, and it was until recently thought to be restricted to East Africa, whereas kdr-w, which involves a leucine-phenylalanine substitution, is associated with resistance in West Africa. In this study, we analyze the frequency and relationship between the kdr genotypes and resistance to type I and type II pyrethroids and DDT by using WHO test kits in both the Forest-M and S molecular forms of An. gambiae in Cameroon. Both kdr-w and kdr-e polymorphisms were found in sympatric An. gambiae, and in many cases in the same mosquito. Kdr-e and kdr-w were detected in both forms, but they were predominant in the S form. Both kdr-e and kdr-w were closely associated with resistance to DDT and weakly associated with resistance to type II pyrethroids. Kdr-w conferred greater resistance to permethrin than kdr-e. We also describe a modified diagnostic designed to detect both resistant alleles simultaneously.

Original languageEnglish (US)
Pages (from-to)260-266
Number of pages7
JournalJournal of Medical Entomology
Volume45
Issue number2
DOIs
StatePublished - Mar 2008

Fingerprint

pesticide resistance
Anopheles gambiae
Pyrethrins
DDT
DDT (pesticide)
Insecticides
pyrethrins
insecticides
mutation
Leucine
Mutation
Insecticide Resistance
Population
Voltage-Gated Sodium Channels
Permethrin
Cameroon
Eastern Africa
Western Africa
Culicidae
Phenylalanine

Keywords

  • Anopheles gambiae
  • Cameroon
  • Insecticide resistance
  • Kdr
  • Pyrethroids

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)

Cite this

Relationship between kdr mutation and resistance to pyrethroid and DDT insecticides in natural populations of Anopheles gambiae. / Reimer, Lisa; Fondjo, Etienne; Patchoké, Salomon; Diallo, Brehima; Lee, Yoosook; Ng, Arash; Ndjemai, Hamadou M.; Atangana, Jean; Traore, Sekou F.; Lanzaro, Gregory C; Cornel, Anthony J.

In: Journal of Medical Entomology, Vol. 45, No. 2, 03.2008, p. 260-266.

Research output: Contribution to journalArticle

Reimer, Lisa ; Fondjo, Etienne ; Patchoké, Salomon ; Diallo, Brehima ; Lee, Yoosook ; Ng, Arash ; Ndjemai, Hamadou M. ; Atangana, Jean ; Traore, Sekou F. ; Lanzaro, Gregory C ; Cornel, Anthony J. / Relationship between kdr mutation and resistance to pyrethroid and DDT insecticides in natural populations of Anopheles gambiae. In: Journal of Medical Entomology. 2008 ; Vol. 45, No. 2. pp. 260-266.
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