Relating mutant genotype to phenotype via quantitative behavior of the NADPH redox cycle in human erythrocytes

Pedro M B M Coelho, Armindo Salvador, Michael A. Savageau

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: The NADPH redox cycle plays a key role in antioxidant protection of human erythrocytes. It consists of two enzymes: glucose-6-phosphate dehydrogenase (G6PD) and glutathione reductase. Over 160 G6PD variants have been characterized and associated with several distinct clinical manifestations. However, the mechanistic link between the genotype and the phenotype remains poorly understood. Methodology/Principal Findings: We address this issue through a novel framework (design space) that integrates information at the genetic, biochemical and clinical levels. Our analysis predicts three qualitatively-distinct phenotypic regions that can be ranked according to fitness. When G6PD variants are analyzed in design space, a correlation is revealed between the phenotypic region and the clinical manifestation: the best region with normal physiology, the second best region with a pathology, and the worst region with a potential lethality. We also show that Plasmodium falciparum, by induction of its own G6PD gene in G6PD-deficient erythrocytes, moves the operation of the cycle to a region of the design space that yields robust performance. Conclusions/Significance: In conclusion, the design space for the NADPH redox cycle, which includes relationships among genotype, phenotype and environment, illuminates the function, design and fitness of the cycle, and its phenotypic regions correlate with the organism's clinical status.

Original languageEnglish (US)
Article numbere13031
JournalPLoS One
Volume5
Issue number9
DOIs
StatePublished - 2010

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Glucosephosphate Dehydrogenase
glucose-6-phosphate 1-dehydrogenase
NADP
NADP (coenzyme)
Oxidation-Reduction
erythrocytes
Erythrocytes
Genotype
Phenotype
phenotype
mutants
genotype
Glutathione Reductase
Physiology
Plasmodium falciparum
Pathology
glutathione-disulfide reductase
molecular genetics
Molecular Biology
physiology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Relating mutant genotype to phenotype via quantitative behavior of the NADPH redox cycle in human erythrocytes. / Coelho, Pedro M B M; Salvador, Armindo; Savageau, Michael A.

In: PLoS One, Vol. 5, No. 9, e13031, 2010.

Research output: Contribution to journalArticle

Coelho, Pedro M B M ; Salvador, Armindo ; Savageau, Michael A. / Relating mutant genotype to phenotype via quantitative behavior of the NADPH redox cycle in human erythrocytes. In: PLoS One. 2010 ; Vol. 5, No. 9.
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