Relatedness of cytotoxins from geographically diverse isolates of Moraxella bovis

John A Angelos, Louise M. Ball

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To determine whether amino acid sequence variation exists in the Moraxella bovis (M. bovis) cytotoxin (MbxA) from geographically diverse M. bovis isolated in the United States, mbxA was amplified and sequenced. The MbxA deduced amino acid sequence from M. bovis originally isolated in California, Washington, North Carolina, and Georgia, as well as reference strains of M. bovis isolated at the National Animal Disease Laboratory, Ames, IA, USA, all encoded a nearly identical 927 amino acid protein. MbxA from two of the four California isolates (SFS 9a and SFS 100a) differed from all other isolates at two sites at which the polar amino acids glutamine (position 666) and asparagine (position 823) were replaced by ionized amino acids glutamic acid and aspartic acid, respectively. Rabbit antiserum to the expressed carboxy terminus (amino acids 590-927) of MbxA from M. bovis (Tifton I) neutralized the hemolytic activity of SFS 9a and SFS 100a. The M. bovis cytotoxin appears to be conserved amongst geographically diverse isolates of M. bovis from the USA. Antiserum against the carboxy terminus of MbxA common to the majority of isolates neutralized the hemolytic activity of two strains with a divergent MbxA deduced amino acid sequence. Vaccines against IBK that incorporate MbxA as antigen may offer protection against geographically diverse strains of M. bovis.

Original languageEnglish (US)
Pages (from-to)382-386
Number of pages5
JournalVeterinary Microbiology
Volume124
Issue number3-4
DOIs
StatePublished - Oct 6 2007

Fingerprint

Moraxella (Moraxella) bovis
Moraxella bovis
cytotoxins
Cytotoxins
Amino Acids
Amino Acid Sequence
amino acids
amino acid sequences
antiserum
Immune Sera
Animal Diseases
Asparagine
animal diseases
asparagine
aspartic acid
Glutamine
glutamic acid
Aspartic Acid
glutamine
Glutamic Acid

Keywords

  • Cytotoxin
  • Infectious bovine keratoconjunctivitis
  • MbxA
  • Moraxella bovis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Relatedness of cytotoxins from geographically diverse isolates of Moraxella bovis. / Angelos, John A; Ball, Louise M.

In: Veterinary Microbiology, Vol. 124, No. 3-4, 06.10.2007, p. 382-386.

Research output: Contribution to journalArticle

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