Reinnervation of laryngeal muscles: A study of changes in myosin heavy chain expression

Paul J. Kingham, Martin A. Birchall, Rachel Burt, Alan Jones, Giorgio Terenghi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Direct repair of the recurrent laryngeal nerve (RLN) results in synkinesis and compromised laryngeal function. We have therefore developed a pig model to investigate whether anastomosis of the phrenic nerve with the abductor branch of the RLN leads to specific reinnervation of abductor muscles. Expression of myosin heavy chain protein (MyHC), a marker of appropriate reinnervation, was determined in the posterior cricoarytenoid (PCA) abductor and thyroarytenoid (TA) adductor muscles following nerve injury and repair. The denervated PCA muscle exhibited decreased levels of the fast-type MyHC isoforms IIA and IIB, and increased slow-type MyHC expression. Similarly, there was a fall in type IIB levels in the denervated TA muscle but increases in both IIA and slow MyHC. Four months after repair, the MyHC expression in the PCA was near normal, suggesting that our model reduces the risk of synkinesis and ensures the accurate muscle reinnervation required for full functional recovery.

Original languageEnglish (US)
Pages (from-to)761-766
Number of pages6
JournalMuscle and Nerve
Volume32
Issue number6
DOIs
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Laryngeal Muscles
Myosin Heavy Chains
Synkinesis
Recurrent Laryngeal Nerve
Proteins
Muscles
Phrenic Nerve
Protein Isoforms
Swine
Wounds and Injuries

Keywords

  • Denervation
  • Larynx
  • Recurrent laryngeal nerve
  • Synkinesis
  • Transplantation

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Kingham, P. J., Birchall, M. A., Burt, R., Jones, A., & Terenghi, G. (2005). Reinnervation of laryngeal muscles: A study of changes in myosin heavy chain expression. Muscle and Nerve, 32(6), 761-766. https://doi.org/10.1002/mus.20409

Reinnervation of laryngeal muscles : A study of changes in myosin heavy chain expression. / Kingham, Paul J.; Birchall, Martin A.; Burt, Rachel; Jones, Alan; Terenghi, Giorgio.

In: Muscle and Nerve, Vol. 32, No. 6, 12.2005, p. 761-766.

Research output: Contribution to journalArticle

Kingham, PJ, Birchall, MA, Burt, R, Jones, A & Terenghi, G 2005, 'Reinnervation of laryngeal muscles: A study of changes in myosin heavy chain expression', Muscle and Nerve, vol. 32, no. 6, pp. 761-766. https://doi.org/10.1002/mus.20409
Kingham, Paul J. ; Birchall, Martin A. ; Burt, Rachel ; Jones, Alan ; Terenghi, Giorgio. / Reinnervation of laryngeal muscles : A study of changes in myosin heavy chain expression. In: Muscle and Nerve. 2005 ; Vol. 32, No. 6. pp. 761-766.
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