TY - JOUR
T1 - Regulation of Tn5 by the right-repeat proteins
T2 - Control at the level of the transposition reaction?
AU - Isberg, Ralph R.
AU - Lazaar, Aili L.
AU - Syvanen, Michael
PY - 1982
Y1 - 1982
N2 - The transposon Tn5 consists of inverted repeats, called IS50R and IS50L, each of which encode two proteins. We show here that the larger protein encoded on IS50R, protein 1, is absolutely required for transposition. Deletion or insertion mutants that fail to make this protein fail to promote gene movement. In addition, this protein acts in cis preferentially. We also show that the smaller protein encoded on IS50R, protein 2, is competent to inhibit transposition of a Tn5 freshly introduced into the cell on a λ phage. In contrast, the proteins from IS50L possess neither of these two activities. By assaying expression of proteins that are hybrids between β-galactosidase and IS50R proteins, we find that the regulation of transposition cannot be due to the inhibitor repressing synthesis of Tn5 proteins. Control experiments, in which we assay synthesis of IS50 proteins synthesized from a λ::IS50R that has been infected into cells carrying the transposition inhibitor, confirm this conclusion.
AB - The transposon Tn5 consists of inverted repeats, called IS50R and IS50L, each of which encode two proteins. We show here that the larger protein encoded on IS50R, protein 1, is absolutely required for transposition. Deletion or insertion mutants that fail to make this protein fail to promote gene movement. In addition, this protein acts in cis preferentially. We also show that the smaller protein encoded on IS50R, protein 2, is competent to inhibit transposition of a Tn5 freshly introduced into the cell on a λ phage. In contrast, the proteins from IS50L possess neither of these two activities. By assaying expression of proteins that are hybrids between β-galactosidase and IS50R proteins, we find that the regulation of transposition cannot be due to the inhibitor repressing synthesis of Tn5 proteins. Control experiments, in which we assay synthesis of IS50 proteins synthesized from a λ::IS50R that has been infected into cells carrying the transposition inhibitor, confirm this conclusion.
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U2 - 10.1016/0092-8674(82)90293-8
DO - 10.1016/0092-8674(82)90293-8
M3 - Article
C2 - 6291787
AN - SCOPUS:0020197982
VL - 30
SP - 883
EP - 892
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -