Regulation of the cloned L-type cardiac calcium channel by cyclic-AMP-dependent protein kinase

Edward Perez-Reyes, Weilong Yuan, Xiangyang Wei, Donald M Bers

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

Hormones can regulate cardiac L-type Ca2+ channels via cAMP-dependent protein kinase (PKA) phosphorylation. However, regulation of the cloned L-type Ca2+ channel has been difficult to demonstrate conclusively. We stably transfected a human embryonic kidney (HEK-293) cell with the cardiac α1 andβ2 subunits, then examined PKA modulation of the α2+ current. Although forskolin did not increase basal Ca2+ current, the PKA inhibitors, H-89 and Rp-cAMPS, could inhibit basal current. We reversed H-89 inhibition with either forskolin or okadaic acid. We conclude that the channel was phosphorylated under basal conditions, and that inhibition of PKA allowed dephosphorylation. These studies demonstrate that reversible PKA regulation of cloned Ca2+ channels can be studied in HEK-293 cells.

Original languageEnglish (US)
Pages (from-to)119-123
Number of pages5
JournalFEBS Letters
Volume342
Issue number2
DOIs
StatePublished - Apr 4 1994
Externally publishedYes

Keywords

  • Adenosine cyclic monophosphate
  • Calcium channel
  • Clone cell
  • Phosphoprotein phosphatase
  • Protein kinase
  • Recombinant DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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