Abstract
Transforming growth factor-β (TGF-β) stimulates new bone formation when administered locally in vivo. The extracellular matrix protein tenascin-C, which is secreted by osteoblasts but absent from mineralized bone matrix, supports differentiation of cultured osteoblast-like cells. The current study was undertaken to determine whether expression patterns of tenascin-C in TGF-β-treated bone cells are in agreement with a role for this protein as a mediator of TGF-β-stimulated new bone formation. Expression of tenascin-C was investigated by immunohistochemistry in calvarial bones of mice to which TGF-β had been locally administered. Three days after initiation of daily TGF-β treatment, strong staining for tenascin-C was seen in regions where periosteal osteoprogenitor cells were undergoing proliferation in response to TGF-β; by comparison, staining of periosteal surfaces of control bones was weak and discontinuous. Seven days after initiation of a course of five daily injections of TGF-β, tenascin-C staining was still enhanced in treated bones. The ability of TGF-β to regulate expression of tenascin-C in osteoblast-like cells in vitro was investigated using an osteosarcoma-derived cell line (ROS 17/2.8). TGF-β caused a small but significant increase in secretion of tenascin-C into the medium, as determined by a solid phase dot immunoassay quantitated by densitometry. Western and northern blot analysis indicated that TGF-β did not influence the pattern of expression of tenascin-C splice variants. These results indicate that TGF-β stimulates osteoblastic tenascin-C expression and suggest that tenascin-C may act as a mediator of TGF-β-induced new bone formation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 301-307 |
| Number of pages | 7 |
| Journal | Bone |
| Volume | 22 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1998 |
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Keywords
- Bone
- Extrucellular matrix
- Osteoblast
- Tenascin-C
- Transforming growth factor-β
ASJC Scopus subject areas
- Physiology
- Hematology
Cite this
Regulation of tenascin-C expression in bone cells by transforming growth factor-β. / MacKie, E. J.; Abraham, L. A.; Taylor, S. L.; Tucker, Richard P; Murphy, L. I.
In: Bone, Vol. 22, No. 4, 04.1998, p. 301-307.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of tenascin-C expression in bone cells by transforming growth factor-β
AU - MacKie, E. J.
AU - Abraham, L. A.
AU - Taylor, S. L.
AU - Tucker, Richard P
AU - Murphy, L. I.
PY - 1998/4
Y1 - 1998/4
N2 - Transforming growth factor-β (TGF-β) stimulates new bone formation when administered locally in vivo. The extracellular matrix protein tenascin-C, which is secreted by osteoblasts but absent from mineralized bone matrix, supports differentiation of cultured osteoblast-like cells. The current study was undertaken to determine whether expression patterns of tenascin-C in TGF-β-treated bone cells are in agreement with a role for this protein as a mediator of TGF-β-stimulated new bone formation. Expression of tenascin-C was investigated by immunohistochemistry in calvarial bones of mice to which TGF-β had been locally administered. Three days after initiation of daily TGF-β treatment, strong staining for tenascin-C was seen in regions where periosteal osteoprogenitor cells were undergoing proliferation in response to TGF-β; by comparison, staining of periosteal surfaces of control bones was weak and discontinuous. Seven days after initiation of a course of five daily injections of TGF-β, tenascin-C staining was still enhanced in treated bones. The ability of TGF-β to regulate expression of tenascin-C in osteoblast-like cells in vitro was investigated using an osteosarcoma-derived cell line (ROS 17/2.8). TGF-β caused a small but significant increase in secretion of tenascin-C into the medium, as determined by a solid phase dot immunoassay quantitated by densitometry. Western and northern blot analysis indicated that TGF-β did not influence the pattern of expression of tenascin-C splice variants. These results indicate that TGF-β stimulates osteoblastic tenascin-C expression and suggest that tenascin-C may act as a mediator of TGF-β-induced new bone formation.
AB - Transforming growth factor-β (TGF-β) stimulates new bone formation when administered locally in vivo. The extracellular matrix protein tenascin-C, which is secreted by osteoblasts but absent from mineralized bone matrix, supports differentiation of cultured osteoblast-like cells. The current study was undertaken to determine whether expression patterns of tenascin-C in TGF-β-treated bone cells are in agreement with a role for this protein as a mediator of TGF-β-stimulated new bone formation. Expression of tenascin-C was investigated by immunohistochemistry in calvarial bones of mice to which TGF-β had been locally administered. Three days after initiation of daily TGF-β treatment, strong staining for tenascin-C was seen in regions where periosteal osteoprogenitor cells were undergoing proliferation in response to TGF-β; by comparison, staining of periosteal surfaces of control bones was weak and discontinuous. Seven days after initiation of a course of five daily injections of TGF-β, tenascin-C staining was still enhanced in treated bones. The ability of TGF-β to regulate expression of tenascin-C in osteoblast-like cells in vitro was investigated using an osteosarcoma-derived cell line (ROS 17/2.8). TGF-β caused a small but significant increase in secretion of tenascin-C into the medium, as determined by a solid phase dot immunoassay quantitated by densitometry. Western and northern blot analysis indicated that TGF-β did not influence the pattern of expression of tenascin-C splice variants. These results indicate that TGF-β stimulates osteoblastic tenascin-C expression and suggest that tenascin-C may act as a mediator of TGF-β-induced new bone formation.
KW - Bone
KW - Extrucellular matrix
KW - Osteoblast
KW - Tenascin-C
KW - Transforming growth factor-β
UR - http://www.scopus.com/inward/record.url?scp=0032055380&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032055380&partnerID=8YFLogxK
U2 - 10.1016/S8756-3282(97)00297-4
DO - 10.1016/S8756-3282(97)00297-4
M3 - Article
C2 - 9556128
AN - SCOPUS:0032055380
VL - 22
SP - 301
EP - 307
JO - Bone
JF - Bone
SN - 8756-3282
IS - 4
ER -