Regulation of signaling at regions of cell-cell contact by endoplasmic reticulum-bound protein-tyrosine phosphatase 1B

Fawaz Haj, Ola Sabet, Ali Kinkhabwala, Sabine Wimmer-Kleikamp, Vassilis Roukos, Hong Mei Han, Markus Grabenbauer, Martin Bierbaum, Claude Antony, Benjamin G. Neel, Philippe I. Bastiaens

Research output: Contribution to journalArticle

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Abstract

Protein-tyrosine phosphatase 1B (PTP1B) is a ubiquitously expressed PTP that is anchored to the endoplasmic reticulum (ER). PTP1B dephosphorylates activated receptor tyrosine kinases after endocytosis, as they transit past the ER. However, PTP1B also can access some plasma membrane (PM)-bound substrates at points of cell-cell contact. To explore how PTP1B interacts with such substrates, we utilized quantitative cellular imaging approaches and mathematical modeling of protein mobility. We find that the ER network comes in close proximity to the PM at apparently specialized regions of cell-cell contact, enabling PTP1B to engage substrate(s) at these sites. Studies using PTP1B mutants show that the ER anchor plays an important role in restricting its interactions with PM substrates mainly to regions of cell-cell contact. In addition, treatment with PTP1B inhibitor leads to increased tyrosine phosphorylation of EphA2, a PTP1B substrate, specifically at regions of cell-cell contact. Collectively, our results identify PM-proximal sub-regions of the ER as important sites of cellular signaling regulation by PTP1B.

Original languageEnglish (US)
Article numbere36633
JournalPLoS One
Volume7
Issue number5
DOIs
StatePublished - May 24 2012

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Non-Receptor Type 1 Protein Tyrosine Phosphatase
protein-tyrosine-phosphatase
Endoplasmic Reticulum
endoplasmic reticulum
Cell membranes
cells
plasma membrane
Cell Membrane
Substrates
tyrosine
EphA2 Receptor
Cell signaling
cell communication
Phosphorylation
Receptor Protein-Tyrosine Kinases
endocytosis
Endocytosis
Anchors
Tyrosine
phosphorylation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Haj, F., Sabet, O., Kinkhabwala, A., Wimmer-Kleikamp, S., Roukos, V., Han, H. M., ... Bastiaens, P. I. (2012). Regulation of signaling at regions of cell-cell contact by endoplasmic reticulum-bound protein-tyrosine phosphatase 1B. PLoS One, 7(5), [e36633]. https://doi.org/10.1371/journal.pone.0036633

Regulation of signaling at regions of cell-cell contact by endoplasmic reticulum-bound protein-tyrosine phosphatase 1B. / Haj, Fawaz; Sabet, Ola; Kinkhabwala, Ali; Wimmer-Kleikamp, Sabine; Roukos, Vassilis; Han, Hong Mei; Grabenbauer, Markus; Bierbaum, Martin; Antony, Claude; Neel, Benjamin G.; Bastiaens, Philippe I.

In: PLoS One, Vol. 7, No. 5, e36633, 24.05.2012.

Research output: Contribution to journalArticle

Haj, F, Sabet, O, Kinkhabwala, A, Wimmer-Kleikamp, S, Roukos, V, Han, HM, Grabenbauer, M, Bierbaum, M, Antony, C, Neel, BG & Bastiaens, PI 2012, 'Regulation of signaling at regions of cell-cell contact by endoplasmic reticulum-bound protein-tyrosine phosphatase 1B', PLoS One, vol. 7, no. 5, e36633. https://doi.org/10.1371/journal.pone.0036633
Haj, Fawaz ; Sabet, Ola ; Kinkhabwala, Ali ; Wimmer-Kleikamp, Sabine ; Roukos, Vassilis ; Han, Hong Mei ; Grabenbauer, Markus ; Bierbaum, Martin ; Antony, Claude ; Neel, Benjamin G. ; Bastiaens, Philippe I. / Regulation of signaling at regions of cell-cell contact by endoplasmic reticulum-bound protein-tyrosine phosphatase 1B. In: PLoS One. 2012 ; Vol. 7, No. 5.
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