Regulation of Schwann cell nerve growth factor receptor by cyclic adenosine 3',5'-monophosphate

K. Mokuno, G. Sobue, U. R. Reddy, J. Wurzer, B. Kreider, H. Hotta, P. Baron, A. H. Ross, David E Pleasure

Research output: Contribution to journalArticle

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Abstract

Previous studies indicated that Schwann cells in immature nerves express nerve growth factor (NGF) receptors, and that this expression is down regulated during development but re-induced by Wallerian degeneration. It was also shown that immature Schwann cells are induced to express galactocerebroside and other molecules characteristic of mature Schwann cells by either contact with an axon or treatment with the cyclic adenosine 3',5'-monophospate (cAMP) analogues dibutyryl cAMP (dbcAMP) and 8-bromo cAMP or the adenylate cyclase activator forskolin. In the present study, NGF receptors on the surface of cultured Schwann cells were demonstrated by binding of an anti-rat NGF receptor monoclonal antibody or of radioiodinated NGF. Treatment of cultured Schwann cells with cAMP analogues or forskolin resulted in a progressive decrease in both immunoreactive NGF receptors and radioiodinated NGF binding. The cultured Schwann cells contained a polyadenylated RNA species homologous with human melanoma NGF receptor mRNA in sequence and size. The amount of this NGF mRNA was lower in cAMP analogue-treated than in untreated Schwann cells.

Original languageEnglish (US)
Pages (from-to)465-472
Number of pages8
JournalJournal of Neuroscience Research
Volume21
Issue number2-4
StatePublished - 1988
Externally publishedYes

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Nerve Growth Factor Receptor
Schwann Cells
Cyclic AMP
Adenosine
Nerve Growth Factor Receptors
Nerve Growth Factor
Cultured Cells
Colforsin
Messenger RNA
Wallerian Degeneration
Adenylyl Cyclases
Axons
Melanoma
Monoclonal Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Mokuno, K., Sobue, G., Reddy, U. R., Wurzer, J., Kreider, B., Hotta, H., ... Pleasure, D. E. (1988). Regulation of Schwann cell nerve growth factor receptor by cyclic adenosine 3',5'-monophosphate. Journal of Neuroscience Research, 21(2-4), 465-472.

Regulation of Schwann cell nerve growth factor receptor by cyclic adenosine 3',5'-monophosphate. / Mokuno, K.; Sobue, G.; Reddy, U. R.; Wurzer, J.; Kreider, B.; Hotta, H.; Baron, P.; Ross, A. H.; Pleasure, David E.

In: Journal of Neuroscience Research, Vol. 21, No. 2-4, 1988, p. 465-472.

Research output: Contribution to journalArticle

Mokuno, K, Sobue, G, Reddy, UR, Wurzer, J, Kreider, B, Hotta, H, Baron, P, Ross, AH & Pleasure, DE 1988, 'Regulation of Schwann cell nerve growth factor receptor by cyclic adenosine 3',5'-monophosphate', Journal of Neuroscience Research, vol. 21, no. 2-4, pp. 465-472.
Mokuno K, Sobue G, Reddy UR, Wurzer J, Kreider B, Hotta H et al. Regulation of Schwann cell nerve growth factor receptor by cyclic adenosine 3',5'-monophosphate. Journal of Neuroscience Research. 1988;21(2-4):465-472.
Mokuno, K. ; Sobue, G. ; Reddy, U. R. ; Wurzer, J. ; Kreider, B. ; Hotta, H. ; Baron, P. ; Ross, A. H. ; Pleasure, David E. / Regulation of Schwann cell nerve growth factor receptor by cyclic adenosine 3',5'-monophosphate. In: Journal of Neuroscience Research. 1988 ; Vol. 21, No. 2-4. pp. 465-472.
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AU - Baron, P.

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