Regulation of Saccharomyces cerevisiae kinetochores by the type 1 phosphatase Glc7p

Ingrid Sassoon, Fedor F. Severin, Paul D. Andrews, Maria Rita Taba, Ken B. Kaplan, Anthony J. Ashford, Michael J R Stark, Peter K. Sorger, Anthony A. Hyman

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

We have investigated the role of protein phosphorylation in regulation of Saccharomyces cerevisiae kinetochores. By use of phosphatase inhibitors and a type I protein phosphatase mutant (glc7-10), we show that the microtubule binding activity, but not the centromeric DNA-binding activity, of the kinetochore complex is regulated by a balance between a protein kinase and the type 1 protein phosphatase (PP1) encoded by the GLC7 gene. glc7-10 mutant cells exhibit low kinetochore-microtubule binding activity in vitro and a high frequency of chromosome loss in vivo. Specifically, the Ndc10p component of the centromere DNA-binding CBF3 complex is altered by the glc7- 10 mutation; Ndc10p is hyperphosphorylated in glc7-10 extracts. Furthermore, addition of recombinant Ndc10p reconstitutes the microtubule-binding activity of a glc7-10 extract to wild-type levels. Finally, the glc7-10-induced mitotic arrest is abolished in spindle checkpoint mutants, suggesting that defects in kinetochore-microtubule interactions caused by hyperphosphorylation of kinetochore proteins activate the spindle checkpoint.

Original languageEnglish (US)
Pages (from-to)545-555
Number of pages11
JournalGenes and Development
Volume13
Issue number5
StatePublished - Mar 1 1999
Externally publishedYes

Fingerprint

Kinetochores
Phosphoric Monoester Hydrolases
Saccharomyces cerevisiae
Microtubules
Protein Phosphatase 1
Centromere
Phosphoprotein Phosphatases
DNA
Protein Kinases
Proteins
Chromosomes
Phosphorylation
Mutation
Genes

Keywords

  • Checkpoint
  • Kinetochore
  • Mitosis
  • Type 1 protein phosphatase

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Sassoon, I., Severin, F. F., Andrews, P. D., Taba, M. R., Kaplan, K. B., Ashford, A. J., ... Hyman, A. A. (1999). Regulation of Saccharomyces cerevisiae kinetochores by the type 1 phosphatase Glc7p. Genes and Development, 13(5), 545-555.

Regulation of Saccharomyces cerevisiae kinetochores by the type 1 phosphatase Glc7p. / Sassoon, Ingrid; Severin, Fedor F.; Andrews, Paul D.; Taba, Maria Rita; Kaplan, Ken B.; Ashford, Anthony J.; Stark, Michael J R; Sorger, Peter K.; Hyman, Anthony A.

In: Genes and Development, Vol. 13, No. 5, 01.03.1999, p. 545-555.

Research output: Contribution to journalArticle

Sassoon, I, Severin, FF, Andrews, PD, Taba, MR, Kaplan, KB, Ashford, AJ, Stark, MJR, Sorger, PK & Hyman, AA 1999, 'Regulation of Saccharomyces cerevisiae kinetochores by the type 1 phosphatase Glc7p', Genes and Development, vol. 13, no. 5, pp. 545-555.
Sassoon I, Severin FF, Andrews PD, Taba MR, Kaplan KB, Ashford AJ et al. Regulation of Saccharomyces cerevisiae kinetochores by the type 1 phosphatase Glc7p. Genes and Development. 1999 Mar 1;13(5):545-555.
Sassoon, Ingrid ; Severin, Fedor F. ; Andrews, Paul D. ; Taba, Maria Rita ; Kaplan, Ken B. ; Ashford, Anthony J. ; Stark, Michael J R ; Sorger, Peter K. ; Hyman, Anthony A. / Regulation of Saccharomyces cerevisiae kinetochores by the type 1 phosphatase Glc7p. In: Genes and Development. 1999 ; Vol. 13, No. 5. pp. 545-555.
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