Regulation of ribosome biogenesis by the rapamycin-sensitive TOR- signaling pathway in Saccharomyces cerevisiae

Ted Powers, Peter Walter

Research output: Contribution to journalArticle

303 Citations (Scopus)

Abstract

The TOR (target of rapamycin) signal transduction pathway is an important mechanism by which cell growth is controlled in all eucaryotic cells. Specifically, TOR signaling adjusts the protein biosynthetic capacity of cells according to nutrient availability. In mammalian cells, one branch of this pathway controls general translational initiation, whereas a separate branch specifically regulates the translation of ribosomal protein (r- protein) mRNAs. In Saccharomyces cerevisiae, the TOR pathway similarly regulates general translational initiation, but its specific role in the synthesis of ribosomal components is not well understood. Here we demonstrate that in yeast control of ribosome biosynthesis by the TOR pathway is surprisingly complex. In addition to general effects on translational initiation, TOR exerts drastic control over r-protein gene transcription as well as the synthesis and subsequent processing of 35S precursor rRNA. We also find that TOR signaling is a prerequisite for the induction of r- protein gene transcription that occurs in response to improved nutrient conditions. This induction has been shown previously to involve both the Ras- adenylate cyclase as well as the fermentable growth medium-induced pathways, and our results therefore suggest that these three pathways may be intimately linked.

Original languageEnglish (US)
Pages (from-to)987-1000
Number of pages14
JournalMolecular Biology of the Cell
Volume10
Issue number4
StatePublished - Apr 1999

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Sirolimus
Ribosomes
Saccharomyces cerevisiae
Ribosomal Proteins
Food
RNA Precursors
Growth
Recombinant Proteins
Adenylyl Cyclases
Signal Transduction
Yeasts
Messenger RNA
Genes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Regulation of ribosome biogenesis by the rapamycin-sensitive TOR- signaling pathway in Saccharomyces cerevisiae. / Powers, Ted; Walter, Peter.

In: Molecular Biology of the Cell, Vol. 10, No. 4, 04.1999, p. 987-1000.

Research output: Contribution to journalArticle

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