Regulation of RhoA activation and cytoskeletal organization by acetylation

Arzu Ulu, Jeffrey A. Frost

Research output: Contribution to journalComment/debate

2 Citations (Scopus)

Abstract

Rho GTPases regulate cell motility in a large part through control of actin cytoskeletal organization. The activation state of Rho proteins is regulated by a wide variety of guanine nucleotide exchange factors (GEFs) and GTPase activating proteins that are differentially expressed among cell types and disease states. The RhoA specific GEF neuroepithelial transforming gene 1 (Net1) is highly expressed in many cancer cells and stimulates cell motility, invasion and cell spreading in response to a variety of ligands. A key feature of Net1 proteins is that they are sequestered in the nucleus in the absence of a motility stimulus. We have recently found that accumulation of the Net1A isoform outside the nucleus, which is the primary Net1 isoform controlling cell motility, is regulated by its acetylation status. Here, we describe acetylation as a novel mechanism of RhoGEF regulation in cell motility that can be targeted in cancer and metastasis.

Original languageEnglish (US)
Pages (from-to)76-81
Number of pages6
JournalSmall GTPases
Volume7
Issue number2
DOIs
StatePublished - Apr 2 2016
Externally publishedYes

Fingerprint

Acetylation
Cell Movement
Guanine Nucleotide Exchange Factors
Genes
Chemical activation
Oncogenes
Protein Isoforms
Rho Guanine Nucleotide Exchange Factors
GTPase-Activating Proteins
rho GTP-Binding Proteins
Actins
Proteins
Cells
Ligands
Neoplasms
Neoplasm Metastasis

Keywords

  • lysine acetylation
  • metastasis
  • Net1A
  • Rho GTPase
  • RhoGEF

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Regulation of RhoA activation and cytoskeletal organization by acetylation. / Ulu, Arzu; Frost, Jeffrey A.

In: Small GTPases, Vol. 7, No. 2, 02.04.2016, p. 76-81.

Research output: Contribution to journalComment/debate

Ulu, Arzu ; Frost, Jeffrey A. / Regulation of RhoA activation and cytoskeletal organization by acetylation. In: Small GTPases. 2016 ; Vol. 7, No. 2. pp. 76-81.
@article{4d269b5f5f854e10944c6f18a8567842,
title = "Regulation of RhoA activation and cytoskeletal organization by acetylation",
abstract = "Rho GTPases regulate cell motility in a large part through control of actin cytoskeletal organization. The activation state of Rho proteins is regulated by a wide variety of guanine nucleotide exchange factors (GEFs) and GTPase activating proteins that are differentially expressed among cell types and disease states. The RhoA specific GEF neuroepithelial transforming gene 1 (Net1) is highly expressed in many cancer cells and stimulates cell motility, invasion and cell spreading in response to a variety of ligands. A key feature of Net1 proteins is that they are sequestered in the nucleus in the absence of a motility stimulus. We have recently found that accumulation of the Net1A isoform outside the nucleus, which is the primary Net1 isoform controlling cell motility, is regulated by its acetylation status. Here, we describe acetylation as a novel mechanism of RhoGEF regulation in cell motility that can be targeted in cancer and metastasis.",
keywords = "lysine acetylation, metastasis, Net1A, Rho GTPase, RhoGEF",
author = "Arzu Ulu and Frost, {Jeffrey A.}",
year = "2016",
month = "4",
day = "2",
doi = "10.1080/21541248.2016.1171283",
language = "English (US)",
volume = "7",
pages = "76--81",
journal = "Small GTPases",
issn = "2154-1248",
publisher = "Landes Bioscience",
number = "2",

}

TY - JOUR

T1 - Regulation of RhoA activation and cytoskeletal organization by acetylation

AU - Ulu, Arzu

AU - Frost, Jeffrey A.

PY - 2016/4/2

Y1 - 2016/4/2

N2 - Rho GTPases regulate cell motility in a large part through control of actin cytoskeletal organization. The activation state of Rho proteins is regulated by a wide variety of guanine nucleotide exchange factors (GEFs) and GTPase activating proteins that are differentially expressed among cell types and disease states. The RhoA specific GEF neuroepithelial transforming gene 1 (Net1) is highly expressed in many cancer cells and stimulates cell motility, invasion and cell spreading in response to a variety of ligands. A key feature of Net1 proteins is that they are sequestered in the nucleus in the absence of a motility stimulus. We have recently found that accumulation of the Net1A isoform outside the nucleus, which is the primary Net1 isoform controlling cell motility, is regulated by its acetylation status. Here, we describe acetylation as a novel mechanism of RhoGEF regulation in cell motility that can be targeted in cancer and metastasis.

AB - Rho GTPases regulate cell motility in a large part through control of actin cytoskeletal organization. The activation state of Rho proteins is regulated by a wide variety of guanine nucleotide exchange factors (GEFs) and GTPase activating proteins that are differentially expressed among cell types and disease states. The RhoA specific GEF neuroepithelial transforming gene 1 (Net1) is highly expressed in many cancer cells and stimulates cell motility, invasion and cell spreading in response to a variety of ligands. A key feature of Net1 proteins is that they are sequestered in the nucleus in the absence of a motility stimulus. We have recently found that accumulation of the Net1A isoform outside the nucleus, which is the primary Net1 isoform controlling cell motility, is regulated by its acetylation status. Here, we describe acetylation as a novel mechanism of RhoGEF regulation in cell motility that can be targeted in cancer and metastasis.

KW - lysine acetylation

KW - metastasis

KW - Net1A

KW - Rho GTPase

KW - RhoGEF

UR - http://www.scopus.com/inward/record.url?scp=84975256383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84975256383&partnerID=8YFLogxK

U2 - 10.1080/21541248.2016.1171283

DO - 10.1080/21541248.2016.1171283

M3 - Comment/debate

AN - SCOPUS:84975256383

VL - 7

SP - 76

EP - 81

JO - Small GTPases

JF - Small GTPases

SN - 2154-1248

IS - 2

ER -