Regulation of proliferation of embryonic heart mesenchyme: Role of transforming growth factor-β1 and the interstitial matrix

Michael Choy, Margaret T. Armstrong, Peter B. Armstrong

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Proliferation of atrioventricular cushion mesenchyme of the embryonic avian heart maintained in three-dimensional aggregate culture is stimulated by interaction with the interstitial matrix. Chicken serum or transforming growth factor-β1, which stimulates proliferation, induces matrix deposition in regions of the aggregate showing high labeling indices with tritiated thymidine. Dispersed heart mesenchyme interstitial matrix introduced into serum-free culture is incorporated into the aggregate and stimulates cellular proliferation similar to serum or transforming growth factor-β1. Proliferation is reversibly inhibited by the peptide Gly-Arg-Gly-Asp-Ser-Pro. It is suggested that transforming growth factor-β1 stimulates the production of interstitial matrix and that a sufficient stimulus for proliferation in this system is the presence of the matrix, which acts as the adhesive support for cellular anchorage.

Original languageEnglish (US)
Pages (from-to)421-425
Number of pages5
JournalDevelopmental Biology
Volume141
Issue number2
DOIs
StatePublished - 1990

ASJC Scopus subject areas

  • Developmental Biology

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