Regulation of mitogenesis, motogenesis, and tubulogenesis by hepatocyte growth factor in renal collecting duct cells

Lloyd G. Cantley, Elvino J G Barros, Mehul M Gandhi, Michael Rauchman, And Sanjay K Nigam

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Hepatocyte growth factor (HGF) has been implicated in branching tubulogenesis of the developing kidney and in response to renal injury. We therefore examined the effects of HGF on a recently described murine inner medullary collecting duct epithelial cell line (mIMCD-3 cells) in comparison with Madin-Darby canine kidney (MDCK) cells. HGF induced mitosis, scattering, and tubulogenesis in both mIMCD-3 cells and MDCK cells. However, mIMCD-3 cells underwent branching tubulogenesis under matrix conditions that did not support these morphogenetic changes in MDCK cells, suggesting substantial differences in regulation of tubulogenesis in these two cell types. In quiescent mIMCD-3 cells, the high-affinity receptor for HGF, c-met, was expressed in a nonphosphorylated state. After stimulation with HGF, there was a >10- fold increase in receptor tyrosine phosphorylation and selective association with at least two intracellular proteins, including the phosphatidylinositol-3-kinase. Thus mIMCD-3 cells, which undergo HGF- dependent mitosis, scattering, and branching tubulogenesis, express the c-met receptor in a highly regulated state and therefore should make an excellent model for examining the mechanisms of HGF-dependent tubulogenesis in the renal collecting duct.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume267
Issue number2
StatePublished - 1994
Externally publishedYes

Fingerprint

Hepatocyte Growth Factor
Kidney
Madin Darby Canine Kidney Cells
Mitosis
Phosphatidylinositol 3-Kinase
Proto-Oncogene Proteins c-met
Epithelial Cells
Phosphorylation
Cell Line
Wounds and Injuries
Proteins

Keywords

  • c-met
  • inner medullary collecting duct
  • kidney
  • kidney development
  • nephrogenesis
  • phosphatidylinositol-3- kinase
  • scatter factor

ASJC Scopus subject areas

  • Physiology

Cite this

Regulation of mitogenesis, motogenesis, and tubulogenesis by hepatocyte growth factor in renal collecting duct cells. / Cantley, Lloyd G.; Barros, Elvino J G; Gandhi, Mehul M; Rauchman, Michael; Nigam, And Sanjay K.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 267, No. 2, 1994.

Research output: Contribution to journalArticle

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