Regulation of matrix metabolism in equine cartilage explant cultures by interleukin 1

M. H. MacDonald, Susan M Stover, N. H. Willits, H. P. Benton

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Explant cultures were set up, using articular cartilage obtained from metatarsophalangeal joints of 11 horses. Explants from 2 horses were used to determine culture conditions appropriate for tissue viability. The cartilage explants maintained steady-state metabolism of proteoglycans during a 13-day evaluation period. The metabolic response of equine articular cartilage to incubation with recombinant human interleukin 1 (0.01 to 100 ng/ml) was studied, using cartilage obtained from the remaining 9 horses, age of which ranged from 3 months to 20 years. Interleukin 1 induced a dose-dependent release of glycosaminoglycan from the matrix during a 3-day incubation period. It also caused dose-dependent inhibition of glycosaminoglycan synthesis during a 3-hour pulse-labeling period. Examples obtained from older horses were significantly (P _ 0.05) less responsive to interleukin 1, with respect to synthesis and release of glycosaminoglycan.

Original languageEnglish (US)
Pages (from-to)2278-2285
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume53
Issue number12
StatePublished - 1992

Fingerprint

interleukin-1
Interleukin-1
cartilage
Horses
Cartilage
explants
glycosaminoglycans
horses
Glycosaminoglycans
metabolism
Articular Cartilage
Metatarsophalangeal Joint
Tissue Survival
synthesis
proteoglycans
Proteoglycans
dosage
joints (animal)
viability

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Regulation of matrix metabolism in equine cartilage explant cultures by interleukin 1. / MacDonald, M. H.; Stover, Susan M; Willits, N. H.; Benton, H. P.

In: American Journal of Veterinary Research, Vol. 53, No. 12, 1992, p. 2278-2285.

Research output: Contribution to journalArticle

MacDonald, M. H. ; Stover, Susan M ; Willits, N. H. ; Benton, H. P. / Regulation of matrix metabolism in equine cartilage explant cultures by interleukin 1. In: American Journal of Veterinary Research. 1992 ; Vol. 53, No. 12. pp. 2278-2285.
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