Regulation of matrix metabolism in equine cartilage explant cultures by interleukin 1

M. H. MacDonald, Susan M Stover, N. H. Willits, H. P. Benton

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67 Scopus citations


Explant cultures were set up, using articular cartilage obtained from metatarsophalangeal joints of 11 horses. Explants from 2 horses were used to determine culture conditions appropriate for tissue viability. The cartilage explants maintained steady-state metabolism of proteoglycans during a 13-day evaluation period. The metabolic response of equine articular cartilage to incubation with recombinant human interleukin 1 (0.01 to 100 ng/ml) was studied, using cartilage obtained from the remaining 9 horses, age of which ranged from 3 months to 20 years. Interleukin 1 induced a dose-dependent release of glycosaminoglycan from the matrix during a 3-day incubation period. It also caused dose-dependent inhibition of glycosaminoglycan synthesis during a 3-hour pulse-labeling period. Examples obtained from older horses were significantly (P _ 0.05) less responsive to interleukin 1, with respect to synthesis and release of glycosaminoglycan.

Original languageEnglish (US)
Pages (from-to)2278-2285
Number of pages8
JournalAmerican Journal of Veterinary Research
Issue number12
StatePublished - 1992

ASJC Scopus subject areas

  • veterinary(all)


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